CLINICOHEMATOLOGICAL, IMMUNOPHENOTYPING, MOLECULAR PROFILE, AND OVERALL SURVIVAL IMPACT IN ACUTE LYMPHOID LEUKEMIA PATIENTS FROM NORTH INDIA: Survival Impact in ALL

MANOJ KUMAR; MOHIT CHOWDHRY; RAJ NATH MAKROO; DEEPIKA RANI; VANDANA SHARMA; PANKAJ SHARMA

doi:10.22159/ajpcr.2019.v12i9.34312

Authors

MANOJ KUMAR Department of Molecular Biology and Transplant Immunology, Indraprastha Apollo Hospitals, New Delhi, India.
MOHIT CHOWDHRY Department of Molecular Biology and Transplant Immunology, Indraprastha Apollo Hospitals, New Delhi, India.
RAJ NATH MAKROO Department of Molecular Biology and Transplant Immunology, Indraprastha Apollo Hospitals, New Delhi, India.
DEEPIKA RANI Department of Molecular Biology and Transplant Immunology, Indraprastha Apollo Hospitals, New Delhi, India.
VANDANA SHARMA Department of Molecular Biology and Transplant Immunology, Indraprastha Apollo Hospitals, New Delhi, India.
PANKAJ SHARMA Laboratory of Oxidative Stress and Cancer Biology, Centre for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh,

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i9.34312

Keywords:

Cytogenetics, Chromosomal aberrations, Acute lymphoblastic leukemia

Abstract

Objective: Cytogenetic plays an inevitable role in predicting the diagnosis of acute leukemia. The recurrent chromosomal aberrations in acute leukemia have provided critical insights into the pathophysiological mechanism of leukemogenesis. Cytogenetics findings at diagnostics provide important information for decision-making in both childhood and adult acute lymphoblastic leukemia (ALL). The cure rate for ALL is >80% in children and 35% in adults. Despite the therapeutic advances in ALL, several important biological and pathophysiological questions remain to be answered to achieve an accurate diagnosis, timely prognosis, and maximum therapeutic benefit.

Methods: The present study was carried out at tertiary care hospital, New Delhi, India. A total of 144 newly diagnosed ALL patients were analyzed for clinicohematological profile, immunophenotyping, conventional, and molecular cytogenetics.

Results: The study population was found to have normal karyotypes in most of the cases; however, abnormalities also reported. Our study clearly indicates that the application of fluorescence in situ hybridization has increased sensitivity and accuracy for detecting various chromosomal abnormalities, more so with the cryptic rearrangements.

Conclusion: We observed that the prevalence of the molecular subgroup of leukemia with a potential for a favorable clinical outcome (ETV6-RUNX1 and hyperdiploidy) in precursor B-ALL is higher in the North India.

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