PROTECTIVE EFFECT OF ROTENONE AGAINST LIPOPOLYSACCHARIDE AND D-GALACTOSAMINE-INDUCED HEMATOTOXICITY: Hematoprotective efficacy of Rotenone

SAMRAT RAKSHIT; ANJANI VERMA; SATENDRA KUMAR NIRALA; MONIKA BHADAURIA

doi:10.22159/ajpcr.2019.v12i18.34324

Authors

SAMRAT RAKSHIT Department of Zoology, Toxicology and Pharmacology Laboratory, Guru Ghasidas University, Bilaspur, Chhattisgarh, India.
ANJANI VERMA Department of Zoology, Toxicology and Pharmacology Laboratory, Guru Ghasidas University, Bilaspur, Chhattisgarh, India.
SATENDRA KUMAR NIRALA Department of Rural Technology and Social Development, Laboratory of Natural Products, Guru Ghasidas University, Bilaspur, Chhattisgarh, India.
MONIKA BHADAURIA Department of Zoology, Toxicology and Pharmacology Laboratory, Guru Ghasidas University, Bilaspur, Chhattisgarh, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i18.34324

Keywords:

Rotenone, Hematology, Serum lipid, Lipopolysaccharide, D-galactosamine

Abstract

Objective: The aim of the present study was to investigate the protective efficacy of rotenone against lipopolysaccharide (LPS) and D-galactosamine (D-GalN)-induced altered hematology.

Methods: Hematotoxicity was induced by coinjection of LPS (50 μg/kg i.p.) and D-GalN (300 mg/kg i.p.). Rotenone (5, 10 and 20 mg/kg p.o.) was administered for 6 days as a pre-treatment. Blood was collected through puncturing the retro-orbital sinus to analyze blood parameter. Serum was separated to analyze glucose, triglyceride, and cholesterol.

Results: The present study revealed decreased in red blood cells, platelets, hemoglobin, and hematocrit value while a significant increase in white blood cells, lymphocyte, and monocytes were observed in LPS and D-GalN treated rats. LPS and D-GalN administration significantly decrease glucose level while serum lipid profile (triglycerides and cholesterol level) were increased significantly at 5% level of significance. LPS and D-GalN-induced altered hematological and serological variables were restored toward control by rotenone pretreatment for 6 days in dose-dependent manner.

Conclusion: It can be said that LPS and D-GalN administration resulted in alteration of various hematological parameters and rotenone at 20 mg/kg dose restored significant alteration toward control due to the presence of antioxidant activity of rotenone.

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