EVALUATION OF ANTIPYRETIC AND ANTIULCER ACTIVITY OF ETHANOLIC EXTRACT OF LEAVES OF TREMA ORIENTALIS L. IN ALBINO WISTAR RATS

VANITA G KANASE; JYOTI SINGH

doi:10.22159/ajpcr.2019.v12i12.35808

Authors

VANITA G KANASE Department of Pharmacology, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.
JYOTI SINGH Department of Pharmacology, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i12.35808

Keywords:

Trema orientalis L, Antiulcer activity, Ethanol-induced ulcer, Ulcer index

Abstract

Objectives: The purpose of the present study was aimed at evaluating the antipyretic and antiulcer activity on leaves of Trema orientalis L. (family Cannabaceae) on albino Wistar rats. The antipyretic and antiulcer activity of the ethanolic extract of the T. orientalis L. leaves in three different concentrations (100, 200, and 300 mg/kg) was compared with standard paracetamol and pantoprazole, which was evaluated by employing Brewer’s yeast-induced pyrexia and ethanol-induced ulcer model. The biochemical parameters such as the volume of gastric juice secretion, pH, total acidity, ulcer index, percentage protection, and lipid peroxidation were also studied.

Methods: T. orientalis leaves were extracted with ethanol by the Soxhlet extraction method. The dried extract was used for further phytochemical and pharmacological analysis. The antipyretic effect was studied using Brewer’s yeast-induced pyrexia. The ethanol-induced ulcer model was used to study the antiulcer effect.

Results: The percentage yield of ethanolic extract of T. orientalis leaves was found to be 6.71% w/w, respectively. The extract showed significant antipyretic and antiulcer effect when compared with standard paracetamol and pantoprazole.

Conclusion: The ethanolic extract of T. orientalis has significant antipyretic and antiulcer action.

Downloads

Download data is not yet available.

References

Harbison S, Dempsey D. Peptic ulcer disease. Curr Probl Surg 2005;42:346-454.

Sharma D, Bhatt S. A comprehensive review on ulcer healing potential of medicinal plants. Int J Pharm Pharm Sci 2014;6:201.

Dimo T, Ngueguim FT, Kamtchouing P, Dongo E, Tan PV. Glucose lowering efficacy of the aqueous stem bark extract of Trema orientalis (Linn) blume in normal and streptozotocin diabetic rats. Pharmazie 2006;61:233-6.

Jiji KN, Pramod C, Prasad BS, Muralidharan P, BaidMetha CL. Evaluation of antidiabetic activity of ethanolic extract of Trema orientalis (L.) blume leaves. IOSR J Pharm Biol Sci 2016;11:17-26.

Abiodun O, Gbotosho G, Ajaiyeoba E, Happi T, Falade M, Wittlin S, et al. In vitro antiplasmodial activity and toxicity assessment of some plants from Nigerian ethnomedicine. Pharm Biol 2011;49:9-14.

Orwa C, Mutua A, Kindt R, Jamnadass R, Simons A. Agroforestree Database: A Tree Reference and Selection Guide Version 4.0, 2009. Available from: http://www.worldagroforestry.org/af/treebd. [Last accessed on 2009 Feb 18].

Olanlokun JO, David OM, Afolayan AJ. In vitro antiplasmodial activity and prophylactic potentials of extract and fractions of Trema orientalis (Linn.) stem bark. BMC Complement Altern Med 2017;17:407.

Uddin SN, Uddin KM, Ahmed F. Analgesic and antidiarrhoeal activities of Trema orientalis Linn in mice. Orient Pharm Exp Med 2008;8:187-91.

Panchal HS, Master SM, Shah UD, Saluja AK, Dholwani KK. Anti-convulsion activity of leaf of Trema orientalis. Int J Pharm Res 2010;2:53-5.

Barbera R, Trovato A, Rapisarda A, Ragusa S. Analgesic and antiinflammatory activity in acute and chronic conditions of Trema guineense (schum. etThonn.) Ficalho and Trema micrantha Blume extracts in rodents. Phytother Res 1992;6:146-8.

Chowdhury A, Islam MS. Antibacterial activity of Trema orientalis. Dhaka Univ J Pharm Sci 2004;3:115-7.

McGaw LJ, Jäger AK, van Staden J. Antibacterial, anthelmintic and anti-amoebic activity in South African medicinal plants. J Ethnopharmacol 2000;72:247-63.

Diehl MS, Atindehou KK, Téré H, Betschart B. Prospect for anthelminthic plants in the ivory coast using ethnobotanical criteria. J Ethnopharmacol 2004;95:277-84.

Mpiana PT, Ngbolua KN, Mudogo V, Tshibangu DS, Atibu EK, Tshilanda DD, et al. Anti sickle erythrocytes haemolysis properties and inhibitory effect of anthocyanins extracts of Trema orientalis on the aggregation of human deoxyhemoglobin S in vitro. J Med Sci 2011;11:129-37.

Salprima YS, Eka A, Sri N, Syalfinaf M, Anggria MS, Fatan U. Iron chelating and antiradical activity of kayumanik leaves (Trema orientalis). Indo J Chem 2011;11:196-9.

Hossain E, Mandal SC, Gupta JK. Phytochemical screening and in-vivo antipyretic activity of the methanol leaf-extract of Bombax malabaricum DC (Bombacaceae). Trop J Pharm Res 2011;10:55-60.

Busia K. Ghana Herbal Pharmacopeia Accra. Ghana: The Adventist Press; 1992. p. 141-3.

Borrelli F, Izzo AA. The plant kingdom as a source of anti-ulcer remedies. Phytother Res 2000;14:581-91.

Di Carlo G, Mascolo N, Izzo AA, Capasso F. Flavonoids: Old and new aspects of a class of natural therapeutic drugs. Life Sci 1999;65:337-53.

Galati EM, Monforte MT, Tripodo MM, d’Aquino A, Mondello MR. Antiulcer activity of Opuntia ficus indica (L.) mill. (Cactaceae): Ultrastructural study. J Ethnopharmacol 2001;76:1-9.

Khandelwal K. Practical Pharmacognosy. Maharashtra: Nirali Prakashan; 2008.

Akapa TC, Kehinde AO, Beatrice OO, Joseph O. Antipyretic activity of Abutilon mauritianum (Jacq.) roots in Wistar rats. Int J Pharma Sci Res 2014;5:42-6.

Priya S, Nethaji S. Antipyretic Activity of ethanolic extract of leaf and bark of Diospyros virginiana in yeast induced pyrexia. Int J Pharm Pharm Sci 2015;7:502-4.

Loux JJ, Deplama PD, Yankel SL. Antipyretic testing of aspirin in rats. Toxicol Appl Pharmacol 1972;22:672-5.

Sathish R, Kumar PS, Natarajan K, Sridhar N. Hepatoprotective and anti pyretic activities of methanolic extract of Butea monosperma Lam stem bark in Wister rats. Asian J Pharm Res 2011;1:130-3.

Surwase US, Shaikh GH, Patil SM, Hunsnalkar SG, Chaudhary RY. Phytochemical investigation and preliminary pharmacological screening of Alstonia scholaris R. Br. As an antipyretic. Asian J Res Chem 2009;2:304-5.

Valli G, Vijayalakshmi R, Vasanthi A, Thirupathi AT. Studies of antipyretic and anti-inflammatory activities of odina woodier leaves extracts. Res J Pharmacol Pharmacodyn 2011;3:263-7.

Patil SB, Chavan GM, Ghodke DS, Naikwade NS, Magdum CS. Screening of some indigenous plants for their antipyretic activity. Res J Pharmacol Pharmacodyn 2009;1:143-4.

Kumar B, Singh A, Kaur M. Comparative antidiarrheal and antiulcer effect of the aqueous and ethanolic stem bark extracts of Tinospora cordifolia in rats. J Adv Pharm Tech Res 2014;5:122.

Parsons ME. Pantoprazole, a new proton-pump inhibitor, has a precise and predictable profile of activity. Eur J Gastroenterol Hepatol 1996;8 Suppl 1:S15-20.

Shin JM, Sachs G. Pharmacology of proton pump inhibitors. Curr Gastroenterol Rep 2008;10:528-34.

Oates PJ, Hakkinen JP. Studies on the mechanism of ethanol-induced gastric damage in rats. Gastroenterology 1988;94:10-21.

Rathnakumar K, Jaikumar S, Sengottuvelu S. Antiulcer activity of Euphorbia hirta against experimentally induced ulcer in rats. Asian J Pharm Res 2013;3:122-4.

Mahurkar N, Hasan SMS. Antiulcer activity of Commicarpus Chinensis in ethanol and aspirin induced ulcers. Asian J Pharm Res 2014;4:119-22.

Girish C, Vineela S, Reddy YN, Reddy OV, Rajasekhar KK, Shankarananth V. Evaluation of antiulcer activity of Coccinia grandis leaves. Res J Pharmacol Pharmacodyn 2011;3:92-5.

Kumar V, Bhat Z, Kumar D, Khan N, Chashoo I, Ara I. Gastroprotective effect of leaf extracts of Basella alba var. Alba against experimental gastric ulcers in rats. Rev Bras Farmacognosia 2012;22:657-62.

Divakar M, Devi S. Antiulcer activity of Wrightia tinctoria (Roxb.) R. Br. Pharm Sin 2018;2:355-60.

Dhanaraj TS, Murugaiah K, Sarmila. Antiulcer activity of Mukia maderaspatana on stress induced in rats. Asian J Res Pharm Sci 2012;2:52-4.

Mahurkar SM, Hasan SU, Malpani AA. Gastroprotective influence of Vitamin C and its combination with rabeprazole in pylorus ligation induced ulcers. Asian J Res Pharm Sci 2014;4:71-6.

Ganguly AK, Bhatnagar OP. Effect of bilateral adrenalectomy on production of restraint ulcers in the stomach of albino rats. Can J Physiol Pharmacol 1973;51:748-50.

Malairajan P, Gopalakrishnan G, Narasimhan S, Veni KJ, Kavimani S. Anti-ulcer activity of crude alcoholic extract of Toona ciliata Roemer (heart wood). J Ethnopharmacol 2007;110:348-51.

Rathod S, Motghare V, Deshmukh V. Study of antiulcer activity of angiotensin receptor antagonists in experimentally induced gastric ulcers in rats. Int J Pharm Sci Res 2014;5:502-7.

Swathi D, Prasad KV. Antioxidant and antiulcer potential of ethanolic extract of bark of Myrica esculenta in pyloric ligation ulcer model. Int J Pharm Pharm Sci 2015;7:195-8.

Ghaisas MM, Sharma S, Ganu GP, Limaye RP. Antiulcer activity of Colebrookea oppositifolia SM. Res J Pharmacol Pharmacodyn 2010;2:66-70.