Safety evaluation of petroleum ether, acetone, and hydroalcoholic extracts of Holoptelea integrifolia by oral administration in Wistar rats
Abstract
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Objective: There are no reports on safety information of Holoptelea integrifolia (Roxb.) Planch. bark extracts by oral administration for long duration.
This study is planned to add information on the safety margin of H. integrifolia.
Methods: Acute oral toxicity study was carried out in the consideration of OECD guideline 425. Animals were observed for mortality, morbidity,
behavior and toxic symptoms for 14 consecutive days. Sub-acute toxicity was planned by employing the OECD guideline 407. Dose 50, 100
and 200 mg/kg of the extracts were administered orally, daily for 28 consecutive days. Animals were observed for toxicity sign, morbidity and
mortality. Body weight, relative organ weight, biochemical, hematological parameters and histopathology of vital organs like liver and kidney
were evaluated.
Results: In the acute oral toxicity study, all the extracts were found to be safe at 1000 mg/kg. However, out of three extracts, petroleum ether extract
of the plant showed mild damage to liver and kidney in sub-acute toxicity studies.
Conclusion: Hydroalcoholic extract of H. integrifolia is found to be safe, compared to the acetone and petroleum ether extracts for the long-term use.
Keywords: Holoptelea integrifolia, Bark, Acute toxicity, Chronic toxicity
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References
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