PHYTOCHEMICAL COMPOUNDS AND ANTIOXIDANT ACTIVITY OF YELLOW VELVETLEAF FRUIT (LIMNOCHARIS FLAVA) EXTRACT

ACE BAEHAKI; SHANTI DWITA LESTARI; NORYATI SIREGAR

doi:10.22159/ajpcr.2020.v13i2.36136

Authors

ACE BAEHAKI Study Program of Fisheries Product Technology, Faculty of Agriculture, Sriwijaya University, Indralaya, South Sumatera, Indonesia.
SHANTI DWITA LESTARI Study Program of Fisheries Product Technology, Faculty of Agriculture, Sriwijaya University, Indralaya, South Sumatera, Indonesia.
NORYATI SIREGAR Study Program of Fisheries Product Technology, Faculty of Agriculture, Sriwijaya University, Indralaya, South Sumatera, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i2.36136

Keywords:

Antioxidant, Phytochemical, Reducing ability, Yellow velvetleaf fruit

Abstract

Objective: The purpose of this research was to observe the content of phytochemical compound and antioxidant activity of yellow velvetleaf fruit (Limnocharis flava) extract.

Methods: Research consisted of several stages, including sampling, sample preparation, sample extraction, calculation of yield extract, phytochemical analysis (flavonoids, steroids, alkaloids, saponins, and triterpenoids), and antioxidant activity assessment using 2’,2’-diphenyl-1-picrylhydrazyl and iron-reducing power method.

Results: The n-hexane and methanol extract contained flavonoids, saponins, and triterpenoids, while flavonoids and triterpenoids were detected on ethyl acetate extract. The IC50 of yellow velvetleaf extract with n-hexane, ethyl acetate, and methanol was 3321.67 ppm, 1439.24 ppm, and 96.0 ppm, respectively. The methanol extract had the highest iron-reducing power with the average absorbance of 0.588 followed by the ethyl acetate extract with the average absorbance of 0.195 and n-hexane extract had lowest with the average absorbance of 0.171.

Conclusion: The best solvent to extract the yellow velvetleaf and have highest antioxidant activity was methanol solvent.

Downloads

Download data is not yet available.

References

Cheung LM, Cheung PC, Ooi VE. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem 2003;81:249-55.

Inganakal TS, Swamy PL. Evaluation of in vitro antioxidant activity of a triterepne isolated from Madhuca longifolia L leaves. Int J Pharm Pharm Sci 2013;5:389-91.

Baehaki A, Widiastuti I, Herpandi, Jannah N. Antioxidant activity of extract of Halodule pinifolia seagrass from solvents with differebt polarities. Orient J Chem 2017;33:181-5.

Supriadi A, Baehaki A, Pratama MC. Antibacterial activity of methanol extract from seagrass of Halodule uninervis in the Coastal of Lampung. Der Pharm Lett 2016;8:77-9.

Baehaki A, Supriadi A, Pratama MC. Antioxidant activity of methanol extract of Halodule uninervis seagrass from the Coastal of Lampung, Indonesia. Res J Pharm Biol Chem Res 2016;7:1173-77.

Baehaki A, Lestari SD, Apriyanti W. Pytochemical screening and antioxidant activity of seed extract of water plant (Nymphaea stellata and Nelumbo nucifera). J Chem Pharm Res 2015;7:221-24.

Baehaki A, Herpandi, Putra AA. Antibacterial activity of exttact from swamp plant, Eleocharis dulcis. Orient J Chem 2018;34:573-5.

Jacoeb AM, Abdullah A, Rusydi R. Microscopic characteristics and composition of yellow velvetleaf (Limnocharis flava) plants from Situ Gede Bogor. J Sumberdaya Perairan 2010;4:1-6.

Abhilash PC, Pandey VC, Srivastava P, Rakesh PS, Chandran S, Singh N, et al. Phytofiltration of cadmium from water by Limnocharis flava (L.) buchenau grown in free-floating culture system. J Hazard Mater 2009;170:791-7.

Rusydi R. Potential of yellow velvetleaf (Limnocharis flava) as protein source for fish feed. Acta Aquat 2014;1:36-8.

Maisuthisakul P, Pasuk S, Ritthiruangdej P. Relationship between antioxidant properties and chemical composition of some Thai plants. J Food Compost Anal 2008;21:229-40.

Harborne JB. Phytochemical Methods. 2nd ed. New York: Chapman and Hall; 1987.

Hanani E, Moneim B, Sekarini R. Identification of antioxidant compounds in the sponge Callispongia sp of the thousand Islands. Magazine Pharm Sci 2005;2:127-33.

Middleton E, Kandaswami C, dan Theoharides TC. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease and cancer. Pharmacol Rev 2000;52:673-751.

Lotito SB, Fraga CG. Catechins delay lipid oxidation and alpha-tocopherol and beta-carotene depletion following ascorbate depletion in human plasma. Proc Soc Exp Biol Med 2000;225:32-8.

Goldberg G. Plants: Diet and Health. Iowa State, USA: Blackwell Publishing Company; 2003.

Trease GE, Evans WC. Pharmalognosy. 14th ed. London: Harcourt Publishers Limited; 2000.