EXAMINATION OF IN VITRO WOUND HEALING ANTIMICROBIAL ACTIVITY OF EXTRACT OF SELECTED MEDICINAL PLANTS
DOI:
https://doi.org/10.22159/ajpcr.2020.v13i2.36475Keywords:
Extraction, Hemidesmus indicus, Wound healing, Ziziphus rugosa, Zone of inhibitionAbstract
Objective: The objective of this study was to discover and examine the in vitro wound healing activity of selected medicinal plants against common wound infecting microorganisms.
Methods: Ziziphus rugosa and Hemidesmus indicus plant parts were used for aqueous and solvent extract preparation, maceration technique was followed. In vitro antibiotic test has been done using disc diffusion method.
Results: Maximum yield for aqueous extract was observed in Z. rugosa bark sample (50.6%) and minimum yield was noted in H. indicus leaf extract in (45.7%) and maximum yield in solvent extract was observed in methanol extract of Z. rugosa (37.5%) whereas minimum yield was observed in of acetone extract of leaves of Z. rugosa (25%). For all sample extracts tested, leaf extract of acetone and methanol extract of Z. rugosa and H. indicus showed the highest antibiotic zone of inhibition of 15 mm and 12 mm, whereas lowest zone of inhibition was observed in aqueous bark extract of Z. rugosa (7 mm).
Conclusion: Crude aqueous and solvent extract of selected plant were showed that promising results have a wound healing aid, efficacy of which could be further improved by studying and practicing more advanced extraction procedure for future prospectus.
Downloads
References
Sathishkumar. Ethnnomedicinal plants of Gopalswamy hills, Western Ghats, Coimbatore District, Tamil Nadu. Int J Plant Anim Environ Sci 2019;9:6-12.
Vinayaka KS, Ravindra P, Kumaraswamy HG. Nutritive composition of fruit of Ziziphus rugosa Lam. Res Artic 2011;2:20-4.
Banerjee A, Ganguly S. Medicinal importance of Hemidesmus indicus: A review on its utilities from ancient ayurveda to 20th century. Adv Biores 2014;5:208-13.
Weissner W. Anantamul (Hemidesmus indicus): A review of biomedical studies and US products. Ayurveda J Health 2014;12:40-52.
Kekuda TR, Raghavendra HL, Vinayaka KS. Evaluation of pericarp and seed extract of Ziziphus rugosa Lam. for cytotoxic activity. Int J Pharm Biol Arch 2011;2:887-90.
Chatterjee S, Banerjee A, Chandra I. Hemidesmus indicus: A rich source of herbal medicine. Med Aromat Plants 2014;3:e155.
Krishnamurthy SR, Sarala P. Determination of nutritive value of Ziziphus rugosa Lamk.: A famine edible fruit and medicinal plant of Western Ghats. Indian J Nat Prod Resour 2012;3:20-7.
Bulbul IJ, Khan MF, Rashid MA. Analgesic and central nervous system depressant activities of methanol extract of Ziziphus rugosa Lam. Leaves. Afr J Pharm Pharmacol 2016;10:849-53.
Abhimany Y, Pratiksha S. Analgesic and anti-inflammatory activities of Ziziphus rugosa root barks. J Chem Pharm Res 2010;2:255-9.
Njimoh DL, Assob JC, Mokake SE, Nyhalah DJ, Yinda CK, Sandjon B. Antimicrobial activities of a plethora of medicinal plant extracts and hydrolates against human pathogens and their potential to reverse antibiotic resistance. Int J Microbiol 2015;2015:547156.
Saif MM, Al-Fakih AA, Hassan MA. Antibacterial activity of selected plant (aqueous and methanolic) extracts against some pathogenic bacteria. J Pharm Phytochem 2017;6:1929-35.
Prashith KT, Vinayaka KS, Mallikarjun N, Bharath AC, Shailendra KB, Rakesh KM, et al. Antibacterial, insecticidal and free radical scavenging activity of methanol extract of Ziziphus rugosa Lam.(Rhamnaceae) fruit pericarp. Pharm J 2011;2:65-9.
Akinyemi KO, Oluwa OK, Omomigbehin EO. Antimicrobial activity of crude extracts of three medicinal plants used in South-West Nigerian folk medicine on some food borne bacterial pathogens. Afr J Tradit Complement Alternat Med 2006;3:13-22.
De Zoysa MH, Rathnayake H, Hewawasam RP, Wijayaratne WM. Determination of in vitro antimicrobial activity of five Sri Lankan medicinal plants against selected human pathogenic bacteria. Int J Microbiol 2019;2019:15.
Shoeb M, Mir M, Nahar N, Mosihuzzaman M. Biological screening of Ziziphus rugosa and Ziziphus oenoplia extractives. Dhaka Uni J Pharm Sci 2005;4:131-4.
Sameera NS, Mandakini BP. Investigations into the antibacterial activity of Ziziphus mauritiana Lam. and Ziziphus xylopyrus (Retz.) willd. Int Food Res J 2015;22:849.
Darbari S, Agrawal A, Verma P, Rai TP, Garg R, Chaudhary SB. Antzimicrobial activity of root extracts of the medicinal plant Hemidesmus indicus (L.) R. Br. Var. Pubescens (W. And A.) H.K.F. World J Pharm Pharm Sci 2016;5:1556-62.
Kaleem WA, Muhammad N, Khan H, Rauf A. Pharmacological and phytochemical studies of genus Ziziphus. Middle East J Sci Res 2014;21:1243-63.
Kurapati VK, Nishteswar K. Phytochemical and clinical evaluation of Sariba (Hemidesmus indicus) on wound healing. Int Res J Pharm 2012;3:277-81.
Gayathri M, Kannabiran K. Antimicrobial activity of Hemidesmus indicus, Ficus benghalensis and Pterocarpus marsupium roxb. Indian J Pharm Sci 2009;71:578-81.
Boyko N, Tkachev A, Kovalenko A, Pisarev D, Kuznietsova V, Sushchuk N, et al. Phytochemical, microbiological, and technological studies in the field of obtaining a hydroalcoholic extract with antimicrobial activity from the liquorice root. Asian J Pharm Clin Res 2019;12:403-7.
Jacob V, Rajiv P. In vitro analysis: The antimicrobial and antioxidant activity of zinc oxide nanoparticles from Curcuma longa. Asian J Pharm Clin Res 2019;12:200-4.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
