ANTIPROLIFERATIVE ACTIVITY OF ELEPHANTOPUS SCABER MEDIATED SILVER NANOPARTICLES AGAINST MCF-7, A-549, SCC-40, AND COLO-205 HUMAN CANCER CELL LINES

ASHWINI S SHINDE; VIJAY D MENDHULKAR

doi:10.22159/ajpcr.2020.v13i2.36497

Authors

ASHWINI S SHINDE Department of Botany, The Institute of Science, Mumbai, Maharashtra, India.
VIJAY D MENDHULKAR Department of Botany, The Institute of Science, Mumbai, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i2.36497

Keywords:

Silver nanoparticles, Elephantopus scaber, Anticancer, Sulforhodamine B assay, MCF-7, A-549, SCC-40, COLO-205

Abstract

Objective: To unearth the applications of nanotechnology in medicine has become imperative with all the advancements in the technique. In the current study, we have attempted to exploit the anticancer ability of the green synthesized silver nanoparticles (AgNPs).

Methods: The AgNPs were synthesized using 60% methanol (H-MeOH) Elephantopus scaber leaf extract, characterized, and discussed priorly. The effect of AgNPs was studied on the human breast (MCF-7), lung (A-549), oral (SCC-40), and colon (COLO-205) cancer cell lines through sulforhodamine B assay. We also carried out the synergistic activity with standard drug adriamycin (ADR).

Results: According to the results obtained, AgNPs showed good antiproliferative activity with GI50 <10 μg/ml on MCF-7, A-549, and SCC-40 cell lines when compared with the standard drug ADR. However, for COLO-205 cell line, the impact was 17.4 μg/ml and thus the treatment was less effective.

Conclusion: The synergistic effect of AgNPs+ADR was even better for all the four cell lines than that of the AgNPs alone.

Downloads

Download data is not yet available.

Author Biography

VIJAY D MENDHULKAR, Department of Botany, The Institute of Science, Mumbai, Maharashtra, India.

Professor and Head, Department of Botany

References

Kharat SN, Mendhulkar VD. Synthesis, characterization and studies on antioxidant activity of silver nanoparticles using Elephantopus scaber leaf extract. Mater Sci Eng C Mater Biol Appl 2016;62:719-24.

Mallath MK, Taylor DG, Badwe RA, Rath GK, Shanta V, Pramesh CS, et al. The growing burden of cancer in India: Epidemiology and social context. Lancet Oncol 2014;15:e205-12.

Niemeyer CM. Nanobiotechnology. In: Reviews in Cell Biology and Molecular Medicine. Weinheim: Wiley-VCH Verlag GmbH and Co., KGaA; 2006.

Raut RW, Lakkakula JR, Kolekar NS, Mendhulkar VD, Kashid SB. Phytosynthesis of silver nanoparticle using Gliricidia sepium (Jacq.). Curr Nanosci 2009;5:117-22.

Holtz RD, Souza Filho AG, Brocchi M, Martins D, Durán N, Alves OL. Development of nanostructured silver vanadates decorated with silver nanoparticles as a novel antibacterial agent. Nanotechnology 2010;21:185102.

Sriram MI, Kanth SB, Kalishwaralal K, Gurunathan S. Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model. Int J Nanomedicine 2010;5:753-62.

Yadav A, Mendhulkar VD. Antiproliferative activity of Camellia sinensis mediated silver nanoparticles on three different human cancer cell lines. J Cancer Res Ther 2018;14:1316-24.

Vichai V, Kirtikara K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protoc 2006;1:1112-6.

Patil S, Sivaraj R, Rajiv P, Venckatesh R, Seenivasan R. Green synthesis of silver nanoparticle from leaf extract of Aegle marmelos and evaluation of its antibacterial activity. Int J Pharm Pharm Sci 2015;7:169-73.

Amudha CK, Deeba F, Rajarajan P. Characterization and anti-neoplastic potential of phytofabricated silver nanoparticles on human breast cancer cell line (MCF-7). Int J Appl Pharm 2019;11:70-7.

Devi JS, Bhimba BV, Ratnam K. Anticancer activity of silver nanoparticles synthesized by the seaweed Ulva lactuca in vitro. Sci Rep 2012;1:242-8.

Firdhouse MJ, Lalitha P. Biosynthesis of silver nanoparticles and its applications. J Nanotechnol 2015;2015:1-18.

He Y, Du Z, Ma S, Liu Y, Li D, Huang H, et al. Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in. Int J Nanomedicine 2016;11:1879-87.

Nagajyothi PC, Sreekanth TV, Lee JI, Lee KD. Mycosynthesis: Antibacterial, antioxidant and antiproliferative activities of silver nanoparticles synthesized from Inonotus obliquus (Chaga mushroom) extract. J Photochem Photobiol B 2014;130:299-304.

Jadhav K, Deore S, Dhamecha D, Jagwani S, Jalalpure S, Bohara R. Phytosynthesis of silver nanoparticles: Characterization, biocompatibility studies, and anticancer activity. ACS Biomater Sci Eng 2018;4:892-9.

Preethi R, Padma PR. Anticancer activity of silver nanobioconjugates synthesized from Piper betle leaves extract and its active compound eugenol. Int J Pharm Pharm Sci 2016;8:201-5.

Buttacavoli M, Albanese NN, Di Cara G, Alduina R, Faleri C, Gallo M, et al. Anticancer activity of biogenerated silver nanoparticles: An integrated proteomic investigation. Oncotarget 2018;9:9685-705.