• REGINA ANDAYANI Department of Pharmacy, Faculty of Pharmacy, Andalas University, Limau Manis Campus, Padang, West Sumatera, 25163, Indonesia.
  • FITHRIANI ARMIN Department of Pharmacy, Faculty of Pharmacy, Andalas University, Limau Manis Campus, Padang, West Sumatera, 25163, Indonesia.
  • AINUL MARDHIYAH Department of Pharmacy, Faculty of Pharmacy, Andalas University, Limau Manis Campus, Padang, West Sumatera, 25163, Indonesia.



Total phenolic, Antioxidant activity, Garcinia atroviridis Griff ex T Anders, Garcinia mangostana L, Garcinia cowa Roxb


Objectives: Garcinia atroviridis Griff. ex T. Anders., Garcinia mangostana L., and Garcinia cowa Roxb. are plants of the genus Garcinia that has been widely used by the community as a food flavoring, spices, and also as a herbal medicinal ingredient. This research aimed to evaluate the total phenolics and antioxidant activity from three species of Garcinia (G. atroviridis Griff. ex T. Anders., G. mangostana L., and G. cowa Roxb.)

Methods: The total phenolic content (TPC) of the extracts was estimated as Gallic Acid Equivalent by the Folin-Ciocalteu method. Antioxidant activity was assessed using Ferric Reducing Antioxidant Power assay.

Results: The TPC of G. mangostana L. rind extract is higher (31.83±3.70%), than G. cowa Roxb.(4.35±0.17%) and G. atroviridis Griff. ex T. Anders. (2.47±0.42%). Based on the antioxidant activity, G. mangostana L. rind has a higher total antioxidant activity (24.68 μmol Fe(II)/g) than G. cowa Roxb. (18.88±0.12 μM Fe (II)/g and G. atroviridis Griff. ex T. Anders.(17.61±0.05 μM Fe(II)/g).

Conclusion: The results showed that G. mangostana L rind extract contains a higher level of TPC and antioxidant activity among the other rinds. The results obtained indicate that the three samples have the potential to be a source of natural antioxidants. Further studies must be carried out to isolate compounds that have antioxidant activity.


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Houston MC. The role of nutrition, nutraceuticals, vitamins, antioxidants, and minerals in the prevention and treatment of hypertension. Altern Ther Health Med 2013;19 Suppl 1:32-49.

Yegdaneh A, Ghannadi A, Dayani L. Chemical constituents and biological activities of two Iranian Cystoseira species. Res Pharm Sci 2016;11:311-7.

Mesripour A, Rafieian-Kopaei M, Bahrami B. The effects of Anethum graveolens essence on scopolamine-induced memory impairment in mice. Res Pharm Sci 2016;11:145-51.

Wahyuni FS, Siregar F, Dharma S. Uji efek sitotoksik ekstrak etanol akar asam kandis (Garcinia cowa Roxb.) pada mencit putih betina dengan metode micronucleus assay. J Sains dan Teknol Farm 2012;17:98-103.

Baehaki A, Lestari SD, Siregar N. Phytochemical compounds and antioxidant activity of yellow velvetleaf fruit (Limnocharis flava) extract. Asian J Pharm Clin Res 2020;13:55-7.

Nelson SS, Yadav SA. A comparative screening and evaluation of the total phenolics, flavonoids, and antioxidant properties in the seed extracts of Punica granatum L, Psidium guajava L, and Vitis vinifera L. Asian J Pharm Clin Res 2019;12:242-7.

Hengsa MS. Aktivitas Antibakteri Ekstrak Etanol Buah Asam Gelugur (Garcinia atroviridis Griff. Et Anders) Terhadap Staphylococcus aureus dan Shigella dysenteriae Serta Bioautografinya, Skripsi. Surakarta: Fakultas Farmasi Universitas Muhammadiyah Surakarta; 2014.

Andayani R, Wahyuni FS, Wirasti Y, Dachriyanus H. Development and validation of RP-HPLC method for quantitative estimation of α-mangostin in the rind extract and fractions of Garcinia mangostana L. and their cytotoxic activity on T47D breast cancer cell line. Int J Pharm Pharm Sci 2015;7:174-8.

Wahyuni FS, Shaari K, Stanslas J, Lajis NH, Dachriyanus H. Cytotoxic xanthones from the stem bark of Garcinia cowa Roxb. J Chem Pharm Res 2015;7:227-30.

Departemen Kesehatan Republik Indonesia. Farmakope Herbal Indonesia. Jakarta: Departemen Kesehatan Republik Indonesia; 2008.

Harborne JB. Phytochemical Methods: A Guide to Modern Technique of Plant Analysis. London: Chapman and Hall; 1973.

Singleton VL, Rossi JA. Colourimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1956;16:144-58.

Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem 1996;239:70-6.

Suttirak W, Manurakchinakorn S. Total Phenolic Contents and Antioxidant Activities of Mangosteen Peel Extracts. Chonburi, Thailand: Proceedings of the 35th Congress on Science and Technology of Thailand; 2009.

Sharififar F, Dehghn-Nudeh G, Mirtajaldini M. Major flavonoids with antioxidant activity from Teucrium polium L. Food Chem 2009;112:885-8.

Amran AA, Zaiton Z, Faizah O, Morat P. Effects of Garcinia atroviridis on serum profiles and atherosclerotic lesions in the aorta of guinea pigs fed a high cholesterol diet. Singapore Med J 2009;50:295-9.

Esmaeili AK, Taha RM, Mohajer S, Banisalam B. Antioxidant activity and total phenolic and flavonoid content of various solvent extracts from In Vivo and In Vitro grown Trifolium pratense L. (Red Clover). BioMed Res Int 2015;1-11.

Duh PD, Du PC, Yen GC. The action of methanolic extract of mung bean hulls as inhibitors of lipid peroxidation and non-lipid oxidative damage. Food Chem Toxicol 1999;37:1055-61.

Rohman A, Rafi M, Alam G, Muchtaridi M, Windarsih A. Chemical composition and antioxidant studies of underutilized part of mangosteen (Garcinia mangostana L.) fruit. J Appl Pharm Sci 2019;9:47-52.

Ghasemzadeh A, Jaafar HZ, Baghdadi A, Tayebi-Meigooni A. Alpha-mangostin-rich extracts from mangosteen pericarp: Optimization of green extraction protocol and evaluation of the biological activity. Molecules 2018;23:1852-9.



How to Cite

ANDAYANI, R., F. ARMIN, and A. MARDHIYAH. “ ANDERS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 13, no. 8, Aug. 2020, pp. 149-52, doi:10.22159/ajpcr.2020.v13i8.36525.



Original Article(s)