EXTRACTION AND CHARACTERIZATION OF ANTIBACTERIAL PIGMENT FROM ROSEOMONAS GILARDII YP1 STRAIN IN YERCAUD SOIL

SIDDHARTHAN N; SANDHIYA R; HEMALATHA N

doi:10.22159/ajpcr.2020.v13i3.36580

Authors

SIDDHARTHAN N Department of Microbiology, School of Bioscience, Periyar University, Salem, Tamil Nadu, India.
SANDHIYA R Department of Microbiology, School of Bioscience, Periyar University, Salem, Tamil Nadu, India.
HEMALATHA N Department of Microbiology, School of Bioscience, Periyar University, Salem, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i3.36580

Keywords:

Pigment, Roseomonas gilardii, Extraction, Antibacterial activity, Characterization

Abstract

Objective: The current study is focused on antibacterial pigment production from Yercaud hills region soil bacteria.

Methods: In this present study, a soil sample was collected from a coffee plantation in Yercaud, Salem district. The collected soil sample was used to isolate the pigment-producing bacterial strains. The pigments were extracted by acidified ethanol and there used to screen the antibacterial activity against clinical pathogens. The potential antibacterial pigment producer was identifying and the pigment was characterized by ultraviolet spectroscopy, thin-layer chromatography, and Fourier transform infrared (FT-IR) and also evaluate the minimum inhibitory concentration of pigment against clinical pathogens.

Results: The pigment-producing bacterial strains were isolated from Yercaud soil, among the isolates, YP1 was maximum activity against test pathogens. The potential pigment producer was identified as Roseomonas gilardii. The extracted pigments showed the maximum absorbance at 450 nm and their functional groups were identified using FT-IR analysis.

Conclusion: Based on the results, the pigment was extracted from R. gilardii and it may serve as potential antibacterial pigment of interest in the food industries, textile industries in natural colorant, and cosmetic industries.

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Author Biography

SIDDHARTHAN N, Department of Microbiology, School of Bioscience, Periyar University, Salem, Tamil Nadu, India.

Department of Microbiology

References

Cristea D, Vilarem G. Improving light fastness of natural dyes on cotton yarn. Dyes Pigments 2006;70:238-45.

Delgado-Vargas F, Jiménez AR, Paredes-López O. Natural pigments: Carotenoids, anthocyanins, and betalains--characteristics, biosynthesis, processing, and stability. Crit Rev Food Sci Nutr 2000;40:173-289.

Bechtold T, Mussak R. Handbook of Natural Colorants. Chichester: A John Wiley and Sons, Ltd. Publication; 2009.

Galaffu N, Bortlik K, Michel M. An industry perspective on natural food colour stability. Colour additives for foods and beverages. Food Sci Technol Nutr 2015;130:91-130.

Venil CK, Zakaria ZA, Ahmad WA. Bacterial pigments and their applications. Process Biochem 2013;48:1065-79.

Dufosse L, Galaup P, Yaron A, Arad SM. Microorganisms and microalgae as sources of pigments for food use a scientific oddity or an industrial reality. Trends Food Sci Technol 2013;16:389-406.

Venil CK, Lakshmanaperumalsamy P. An insightful overview on microbial pigment prodigiosin. Electron J Biol 2009;5:49-61.

Sinha S, Choubey S, Kumar AA, Bhosale P. Identification characterization of pigment producing bacteria from soil and water and testing of antimicrobial activity of bacterial pigments. Inst J Pharm Sci Rev Res 2017;42:119-24.

Zerrad A, Anissi J, Ghanam J, Sendide K, Hassouni M. Antioxidant and antimicrobial activities of melanin produced by a Pseudomonas balearica strain. J Biotechnol Lett 2014;5:87-94.

Vora JU, Jain NK, Modi HA. Extraction, characterization and application studies of red pigment of halophile Serratia marcescens KH1R-KM035849 isolated from Kharaghoda soil. Int J Pure Appl Biosci 2014;2:160-16.

Mamunur R, Fakruddin M, Mazumdar RM, Kaniz F, Chowdury A. Anti-bacterial activity of pigments isolated from pigment forming soil bacteria. Br J Pharm Res 2014;4:880-94.

Palaniswamy M, Vishnu TS. Isolation and identification of Chromobacterium sp. Asian J Pharm Clin Res 2016;9:253-7.

Holt JG. Bergey’s Manual of Determinative Bacteriology. 9th ed. Baltimore: Lippincott Williams and Wilkins; 1994.

Samyuktha S, Mahajan S. Isolation and identification of pigment producing bacteria and characterization of extracted pigments. Int J Appl Res 2016;2:657-64.

Rajguru SA, Sawant N, Valmiki A, Deshmukh PV. Isoaltion and identification of pigment producing bacterial isolates from different terrestrial habitats in Thane district M.S, India. World J Pharm Pharm Sci 2015;1:618-28.

Krishna S, Krishna G, Prakasha RS, Charya MA. Antibacterial activity of textile fabrics treated with red pigments from marine bacteria. J Marine Biosci 2015;1:11-9.

Gulani C, Bhattacharya S, Das A. Assessment of process parameters influencing the enhanced production of prodigiosin from Serratia marcescens and evaluation of its antimicrobial antioxidant and dyeing potentials. Malaysian J Microbiol 2012;8:116-22.

Elkenawy NM, Yassin AS, Elhifnawy HN, Amin MA. Optimization of prodigiosin production by Serratia marcescens using crude glycerol and enhancing production using gamma radiation. Biotechnol Rep (Amst) 2017;14:47-53.