IN VITRO SHOOT REGENERATION OF SWALLOW ROOT (DECALEPIS HAMILTONII) – A STENO-ENDEMIC RED LISTED MEDICINAL PLANT

Manjula Ranganatha; Annapurna AS; Ashwani Sharma; Nagashree N Rao

doi:10.22159/ajpcr.2020.v13i4.36714

Authors

Manjula Ranganatha Department of Biotechnology, RV College of Engineering, Visvesvaraya Technological University, Belagavi, Karnataka, India.
Annapurna AS Department of Botany, Jnanabharathi Campus, Bangalore University, Bengaluru, Karnataka, India.
Ashwani Sharma Department of Botany, Jnanabharathi Campus, Bangalore University, Bengaluru, Karnataka, India.
Nagashree N Rao Department of Biotechnology, RV College of Engineering, Visvesvaraya Technological University, Belagavi, Karnataka, India.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i4.36714

Keywords:

Regeneration, Decalepis, 2-Hydroxy-4-methoxybenzaldehyde, Apocynaceae, Nil

Abstract

Objective: In vitro shoot regeneration of Decalepis hamiltonii Wight and Arn. is an endangered endemic medicinal plant using biotechnological interventions and to conserve this threatened species.

Methods: In the present study, various explants such as shoot tip, leaf, and nodal segments were inoculated on Murashige and Skoog media augmented with different hormonal regimes of auxin and cytokinin combinations, namely, naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), benzyl adenine (BAP), 6-(γ,γ-Dimethylallylamino)purine (2iP), and triacontanol (TRIA).

Results: Direct regeneration of shoots obtained in 3.0 mg/l 2iP alone and in combination with 0.1 mg/l IAA and/or 1.0 mg/l BAP exhibited the best response with average shootlet length being 6.5±0.17–8.0±0.92 cm, respectively, and percentage response was between 68% and 75%. The callus induced regeneration was obtained from both nodal and leaf explants with maximum response (85%) observed in combination of (2.0 mg/l) 2iP, (1.0 mg/l) IAA and (2.0 mg/l) kinetin with multiple shoots showing mean shoot number of 1.83 and average shootlet length of 6.3±0.19 cm.

Conclusions: The current research provides a competent in vitro propagation method for Decalepis which could be commercialized for developing identical plants with good mass multiplication rate and for better conservation of the germplasm.

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