• ATOLE D Department of Pharmaceutical Chemistry, Government College of Pharmacy, Aurangabad, Maharashtra, India.
  • DEOKATE UA Department of Pharmaceutical Chemistry, Government College of Pharmacy, Aurangabad, Maharashtra, India.



HPLC, Troubleshooting, Baseline, Pharmaceutical industry


For pharmaceutical industry, HPLC is more than 40 years old. Even after instrumental advancements in HPLC problems still arise, troubleshooting still comes. HPLC is the main stay of analytical section nowadays in pharmaceutical industry whether it is API industry or formulation plant. Best approach for troubleshooting HPLC problems is going with systematic way. At the start of quantitative chromatographic analysis, the first parameter of paramount importance is baseline, especially for measuring the area of any given peak. Baseline usually suffers from these errors, namely, high baseline drift, periodic baseline fluctuation, and spikes. This article aims at suggesting some working methodologies which will resolve as well as avoid cases of such errors and failures.


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Author Biography

DEOKATE UA, Department of Pharmaceutical Chemistry, Government College of Pharmacy, Aurangabad, Maharashtra, India.

Associate Professor at Government College Of Pharmacy


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How to Cite

D, A., and D. UA. “INSIGHT INTO BASELINE TROUBLES AND PROBLEM RESOLUTIONS IN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 13, no. 7, July 2020, pp. 35-38, doi:10.22159/ajpcr.2020.v13i7.37336.



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