BACTERIAL DNA EXTRACTION METHOD FOR THE DETECTION OF HELICOBACTER PYLORI FROM HUMAN FECAL SAMPLES
DOI:
https://doi.org/10.22159/ajpcr.2020.v13i6.37344Keywords:
DNA extraction, Helicobacter pylori, Polymerase chain reaction, GastritisAbstract
Objective: The work proposed the implementation of a method of DNA extraction for the detection of the pathogen from 50 stool samples.
Methods: A method of DNA extraction with Chelex resin was applied and then detected by conventional polymerase chain reaction (PCR).
Results: By PCR, 11 samples were positive for Helicobacter pylori.
Conclusions: The Chelex extraction methodology allows obtaining DNA with quality necessary to be detected by PCR, making it a fast methodology for its diagnostic application.
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References
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