BACTERIAL DNA EXTRACTION METHOD FOR THE DETECTION OF HELICOBACTER PYLORI FROM HUMAN FECAL SAMPLES
DOI:
https://doi.org/10.22159/ajpcr.2020.v13i6.37344Keywords:
DNA extraction, Helicobacter pylori, Polymerase chain reaction, GastritisAbstract
Objective: The work proposed the implementation of a method of DNA extraction for the detection of the pathogen from 50 stool samples.
Methods: A method of DNA extraction with Chelex resin was applied and then detected by conventional polymerase chain reaction (PCR).
Results: By PCR, 11 samples were positive for Helicobacter pylori.
Conclusions: The Chelex extraction methodology allows obtaining DNA with quality necessary to be detected by PCR, making it a fast methodology for its diagnostic application.
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References
Frías Ordoñez J, Otero W. Practical issues in diagnostic tests for Helicobacter pylori infection: A narrative review. Rev Gastroenterol Peru 2017;37:246-53.
Bayas-Morejón IF, Tigre-León RA, Ramón-Curay R, Núñez-Torres D. Helicobacter pylori: A pathogen of ample risk to health. In: Roesler BM, editor. Gastritis-new Approaches and Treatments. UK: IntechOpen; 2019. p. 1-13.
Rafeey M, Nikvash S. Detection of Helicobacter pylori antigen in stool samples for diagnosis of infection in children. East Mediterr Health J 2007;13:1067-72.
Morejón FB. Aportaciones a la Epidemiología de Arcobacter y Helicobacter Aplicación de Métodos Moleculares a su Detección e Identificación en Alimentos [Tesis Doctoral]. Valencia: Universidad Politécnica de Valencia; 2016.
Moreno Y, Piqueres P, Alonso JL, Jiménez A, González A, Ferrús MA. Survival and viability of Helicobacter pylori after inoculation into chlorinated drinking water. Water Res 2007;41:3490-96.
Nobel HA. Bio-Rad Laboratories; 2017. Available from: http://www.biorad.com/webmaster/pdfs/9184_chelex.pdf.
Tigre A, Erazo F, Yánez D, Bayas-Morejón F. Phylogenetic analysis of Fusarium oxysporum F strains isolated from strawberry crops Fragaria ananassa Duch in the province of Pichincha (Ecuador). Int J Curr Pharm Res 2020;12:92-5.
Suenaga E, Nakamura H. Evaluation of three methods for effective extraction of DNA from human hair. J Chromatogr B Analyt Technol Biomed Life Sci 2005;820:137-41.
Santiago P, Moreno Y, Ferrús MA. Identification of viable Helicobacter pylori in drinking water supplies by cultural and molecular techniques. Helicobacter 2015;20:252-9.
Butler JM. DNA Extraction from Forensic Samples Using Chelex. New York: CSHL Press; 2007. p. 1.
Universidad de Salamanca. Banco Nacional de ADN Carlos III. Spain: Universidad de Salamanca, BancoAdn; 2004. p. 1.
Ahmed O, Asghar AH, Elhassan M. Comparison of three DNA extraction methods for polymerase chain reaction (PCR) analysis of bacterial genomic DNA. Afr J Microbiol Res 2014;8:598-602.
Devi SG, Fathima AA, Radha S, Arunraj R, Curtis WR, Ramya M. A rapid and economical method for efficient DNA extraction from diverse soils suitable for metagenomic applications. PLoS One 2015;10:e0132441.
García-Cuan A, Miranda A. Evaluation of bacterial DNA extraction methods in fecal samples of patients diagnosed with Helicobacter pylori infection. Biociencias 2018;13:6-7.
Widada H, Rohman A, Jenie R, Sismindari S. Application of graphene oxide on aptamer-based biosensor development for authentication of gelatin. Int J Appl Pharm 2019;11:254-8.
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