• VALARMATHI R Department of Biotechnology, Padmavani College of Arts and Science for Women, Salem, Tamil Nadu, India.
  • NATARAJAN D Department of Biotechnology, Periyar University, Salem, Tamil Nadu, India.



Nil, Dryopteris hirtipes, Pteridophytes, Bioactive compounds


Objective: The objective of this study was to analyze GC–MS analysis of whole plant methanolic extract of Dryopteris hirtipes from Dryopteridaceae family.

Methods: Gas chromatography and mass spectrometry analysis of whole plant extract was carried out with instrument GC–MS.

Results: The methanolic extract of D. hirtipes reveals to identify more known and unknown bioactive compounds. In this study, seven major bioactive compounds were identified such as Stigmast-5-en-3-ol(56.65%), Phytol (5.39%), Lanost -8-en-3-ol-(3 β)(3.18%), Neophytadiene(2.68%), Tri-o-trimethylsilyl N-heptaflurobutryl derivative of terbutaline(2.19%), 1H-Imidazole 2-methanol(1.28%), and 8A-(2,4-Dimethyl-1-nitrilo-pent-2-yl) dioxy)tocopherone(1.0%) and low concentrations of compounds like hexadecanoic acid(0.6%).

Conclusion: These identified compounds are having active pharmacological properties such as antimicrobial property, hypotension, anti-inflammatory, anti-tumor, anti-cancer, anti-hepatitis, analgesic, and antipyretic properties. However, D. hirtipes is a rare pteridophyte and used to cure many diseases, and so there need further studies to isolate and identify the specific active compounds present in it.


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How to Cite

R, V., and N. D. “GAS CHROMATOGRAPHY AND MASS SPECTROMETRY ANALYSIS OF BIOACTIVE COMPOUNDS OF DRYOPTERIS HIRTIPES (BLUMZE) KUNTZE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 13, no. 7, July 2020, pp. 119-22, doi:10.22159/ajpcr.2020.v13i7.37715.



Original Article(s)