PREPARATION OF COLD CREAM AGAINST CLINICAL PATHOGEN USING CARALLUMA ADSCENDENS VAR. ATTENUATA
DOI:
https://doi.org/10.22159/ajpcr.2020.v13i9.37976Keywords:
Formulation, Cold cream, Caralluma adscendens var attenuata, Neosporin, Antibacterial activityAbstract
Objective: A simple formulation of cold cream from methanolic extract Caralluma adscendens var. attenuata (MECA) and their antimicrobial activity was tested against various clinical pathogens, namely, Escherichia coli, methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus faecium, and Candida albicans.
Methods: Methanol extract of these plant extract was prepared by the Soxhlet method. We analyzed phytochemical nature of theses plant, and subsequently, a cream was formulated cold-cream C. adscendens var. attenuata (FCA) different concentration such as FCA 50 mg, FCA 100 mg, and FCA 200 mg. In the present study, aimed to the antimicrobial activity of cold cream was measured by agar well diffusion method, and standard antibiotic Neosporin (market available) cream was used as positive control and dummy cold cream (without-MECA) were used as the negative control.
Results: Phytochemical screening showed that the plant extracts were found a rich source of secondary metabolites. For more, the efficacy of cold cream from MECA extracts to against the clinical pathogen. Positive control Neosporin and 200 mg FCA cream was a highly significant difference in the zone of inhibition when compared to dummy cream. The 200 mg FCA was activity against Escherichia coli, methicillin-resistant Staphylococcus aureus, vancomycin-resistant E. faecium, and C. albicans highly significantly difference (p<0.05) compared FCA 50 mg and FAC 100 mg creams.
Conclusion: The results from this study suggested that the cold cream form base of MECA crude had antimicrobial activity in the different clinical pathogen. They could be used as an alternative source to conventional antimicrobial agents for the treatment of pathological infection.
Downloads
References
Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 2001;109:69-75.
Balloux F, Van Dorp L. Q&A: What are pathogens, and what have they done to and for us? BMC Biol 2017;15:2-6.
Findley K, Grice EA. The skin microbiome: A focus on pathogens and their association with skin disease. PLoS Pathog 2014;10:1-3.
Pujji OJ, Nakarmi KK, Shrestha B, Rai SM, Jeffery SL. The bacteriological profile of burn wound infections at a tertiary burns center in Nepal. J Burn Care Res 2019;40:838-45.
Enioutina EY, Teng L, Fateeva TV, Brown JC, Job KM, Bortnikova VV, et al. Phytotherapy as an alternative to conventional antimicrobials: Combating microbial resistance. Expert Rev Clin Pharmacol 2017;10:1203-14.
Kumar KM, Murshida UC, Thomas B, George S, Balachandran I, Karuppusamy S. Notes on Caralluma adscendens (Roxb.) Haw. var. attenuata (Wight) Grav. & Mayur. (Apocynaceae: Asclepiadoideae). J Threat Taxa 2014;6:6282-6.
Kiranmayee P, Anitha K, Usha R. Isolation and identification of steroid triterpenoids from the polar and non-polar fractions of Caralluma attenuate (Weight) roots. Int J Pharmacogn Phytochem Res 2016; 8:912-29.
Raman N. Phytochemical Methods. New Delhi: New Indian Publishing Agencies; 2006. p. 19.
Shamaki BU, Geidam YA, Abdulrahma F, Ogbe AO, Sandabe UK. Evaluation of phytochemical constituents and in vitro antibacterial activity of organic solvent fractions of Ganoderma lucidum methanolic extract. Int J Med Plant Res 2012;1:26-31.
Carter SJ. Cooper and Gunn’s Dispensing for Pharmaceutical Students. 12th ed. Edinburgh: Churchill Livingstone; 1896. p.159.
Pavan Kumar B, Ashok G, Mohammed I, Ramachandra NM, Rashmi KP. Pharmacognostic evaluation of selected species of Caralluma genus. J Phytopharmacol 2015;4:34-40.
Noreen S. A mini review on a Caralluma tuberculata N. E. Br. uncommon and wild succulents but having exciting pharmacological attributes. Pure Appl Biol 2017;6:748-61.
Khémiri I, Hédi BE, Zouaoui NS, Ben Gdara N, Bitri L. The antimicrobial and wound healing potential of Opuntia ficus indica L. inermis extracted oil from Tunisia. Evid Based Complement Altern Med 2019;2019:1-10.
Prakoso YA, Rini CS, Wirjaatmadja R. Efficacy of Aloe vera, Ananas comosus, and Sansevieria masoniana cream on the skin wound infected with MRSA. Adv Pharmacol Sci 2018;2018:4670569.
De Silva CC, Israni N, Zanwar A, Jagtap A, Leophairatana P, Koberstein JT, et al. “Smart” polymer enhances the efficacy of topical antimicrobial agents. Burns 2019;45:1418-29.
Prabakaran R, Kalimuthu K. Antibacterial activity of the whole plant of Caralluma nilagiriana Kumari et Subba Rao-an endemic medicinal plant species. Int J Pharm Bio Sci 2013;4:42-8.
Packialakshmi N, Naziya S. Phytochemical and antimicrobial screening of the polar and non-polar solvent stem extract of Caralluma fimbriyata. Int J Pure Appl Biosci 2014;2:32-7.
Sahana R, Daniel SC, Sankar SG, Archunan G, Vennison SJ, Sivakumar M. Formulation of bactericidal cold cream against clinical pathogens using Cassia auriculata flower extract-synthesized Ag nanoparticles. Green Chem Lett Rev 2014;7:64-72.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
