THE SENSITIVITY TEST OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM SUSPECT TUBERCULOSIS PATIENTS TO THE SEROMUCOUS OF SNAIL AND CHITOSAN AS AN ALTERNATIVE ANTI-TUBERCULOSIS DRUGS

YUSUP SUBAGIO SUTANTO; MAGDALENA SUTANTO; AGNES SRI HARTI; NONY PUSPAWATI

doi:10.22159/ajpcr.2020.v13i10.38266

Authors

YUSUP SUBAGIO SUTANTO Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Sebelas Maret University of Surakarta, Indonesia.
MAGDALENA SUTANTO District General Hospital of Surakarta City, Indonesia.
AGNES SRI HARTI Departemen of Nursing, Kusuma Husada University of Surakarta, Indonesia.
NONY PUSPAWATI Department of ???, Faculty of Health Science, University of Setia Budi (Universitas Setia Budi), Surakarta, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i10.38266

Keywords:

Sensitivity, Isolates, Mycobacterium tuberculosis, Snail seromucous, Chitosan, Streptomycin, Isoniazid, Rifampicin, Ethambutol

Abstract

Objective: The purpose of this research is to study the sensitivity of Mycobacterium tuberculosis (MTB) isolates from suspect TB patients to seromucous of snail and chitosan as an alternative to anti-TB drugs.

Methods: The research methods include management specimen, freeze-drying of snail seromucous; formulation of dosage preparation; identification of MTB isolates; and sensitivity testing of MTB isolates to snail seromucous, chitosan, and streptomycin, isoniazid, rifampicin, and ethambutol (SIRE).

Results: The characteristics of respondents by sex and age are the majority of male respondents and productive adult age that is 26 years–52 years. MTB isolates used in the study were obtained from the results of the screening of sputum samples of suspect TB patients through microscopic smear examination and molecular rapid test using GeneXpert tools. MTB isolates in patients suspect TB are resistant against seromucous of snails and chitosan that it is compared with SIRE. The dosage of snail seromucous is 8000 mg/l, chitosan 2% is 800 mg/l, and SIRE, respectively (rifampicin 8000 mg/l, isoniazid 20 mg/l, ethambutol 200 mg/l, streptomycin 800 mg/l).

Conclusion: MTB isolates from patients suspect who TB is resistant to seromucous of snail (8000 mg/l) and chitosan (800 mg/l).

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References

Zhuang J, Coates CJ, Zhu H, Zhu P, Wu Z, Xie L. Identification of candidate antimicrobial peptides derived from abalone hemocyanin. Dev Comp Immunol 2015;49:96-102.

Dang VT, Benkendorff K, Green T, Speck P. Marine snails and slugs: A great place to look for antiviral drugs. J Virol 2015;89:8114-8.

Dolashka P, Dolasshki A, Velkova L, Stevanovic S, Molin L, Traldi P, et al. Bioactive compounds isolated from garden snails. J BioSci Biotechnol 2015; ???:147-55.

Mandala AP, Harti AS. Effectiveness of 100% Snail Mucus (Achantina fulica) and 5% Cream Preparation for Long-term Healing Wound Healing (Grade A) in vivo. Surakarta: Final Report, Departement of Nursing, Kusuma Husada Surakarta School of Health Science; 2014.

Harti AS, Nurhidayati A, Handayani D. The potential of rice bran and chito-oligosaccharide as natural prebiotic on traditional tempeh in Indonesia. Int J Biosci Biochem Bioinform 2013;3:654-6.

Harti AS, Nurhidayati A, Handayani D, Estuningsih, Kusumawati HN, Haryati DS. The fortification tempeh of rice bran chitosan as functional food antihy-percholesterolemia in Indonesia. Int J Biosci Biochem Bioinform 2014;4:423-7.

Harti AS, Haryati DS, Sunarto, Setyaningsih W, Yatmihatun S. The potential chito-oligo-saccharide (COS) as natural prebiotic and preservatives on synbiotic tofu in Indonesia. Int J Pharm Med Biol Sci 2015;4:204-8.

Octaviana U, Maryati A, Fatimah S, Harti AS. Chitosan Biomembrane as Sanitary Pads. Jakarta: Final Report of Creativity Students Program, Department High Education Indonesia; 2015.

Harti AS, Sulisetyawati SD, Murharyati A, Oktariani M, Wijayanti IB. The effectiveness of snail slime and chitosan in wound healing. Int J Pharm Med Biol Sci 2016;5:76-80.

Indonesian Pulmonary Doctors Association. Guidelines for Tuberculosis Diagnosis and Management in Indonesia. Jakarta: Indonesian Pulmonary Doctors Association; 2006.

Akhavan R, Meshkat Z, Jamehdar S. Comparing the frequency of Mycobacterium tuberculosis with direct microscopy and culture methods. Jundishapur J Microbiol 2012;6:95-6.

Kanade S, Nataraj G, Suryawanshi R, Mehta P. Utility of MPT 64 antigen detection assay for rapid characterization of mycobacteria in a resource constrained setting. Indian J Tuberc 2012;59:92-6.

Haryanto B. Benefits of TB Ag MPT 64 Immunochromatography test for the Differentiation of Mycobacterium tuberculosis Complex and Mycobacterium non Tuberculosis Complex. Jakarta: Thesis, Educational Program of Clinical Microbiology Specialist in the Faculty of Medicine. University of Indonesia; 2015.

Retnoningrum DS, Roga FK. Mechanisms of molecular level of resistance to several drugs in Mycobacterium tuberculosis. Acta Pharm Indones 2004;29:92-5.

Martin A, Paasch F, Von Groll A, Fissete K, Almeida P, Varaine F, et al. Thin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolates. Int J Tuberc Lung Dis 2009;13:1301-4.

Vilchèze C, Jacobs WR Jr. Resistance to isoniazid and ethionamide in Mycobacterium tuberculosis: Genes, mutations, and causalities. Microbiol Spectr 2014;2:MGM2.

Kiepiela P, Bishop KS, Smith AN, Roux L, York DF. Genomic mutations in the katG, inhA and aphC genes are useful for the prediction of isoniazid resistance in Mycobacterium tuberculosis isolates from Kwazulu Natal, South Africa. Tuber Lung Dis 2000;80:47-56.

Harti AS, Puspawati N, Putriningrum R. Antimicrobial bioactive compounds of snail seromucous as biological response modifier immunostimulator. Microbiol Indones 2019;13:56-63.

Harti AS, Puspawati N, Putriningrum R. Characterization of Physicochemistry, Microbiological and Profile of Seromucous Protein Snails (Achatina fullica Ferussac) as in vitro Biological Response Modifiers. Surakarta: Proceeding the 10th International Seminar of Indonesian Society for Microbiology (10th ISISM) and 12th Congress of the Indonesian Society for Microbiology (12th CISM). PERMI; 2019.

Ulagesan S, Kim HJ. Antibacterial and antifungal activities of proteins extracted from seven different snails. Appl Sci 2018;8:1362.

Anggraeni Y. Preparation and Carcarization of Chitosan-tripolyphosphate Cross-connecting Film that Contains Aystosides as Bioactive Bandages for Wounds. Jakarta: Thesis, Masters Program in Pharmaceutical Sciences, FMIPA University of Indonesia; 2012.

Huda M, Marhamah. Effect of snail mucus (Achantina fulica) on the growth of gram-positive and gram-negative Bacteria. Dep Health Anal Tanjungkarang Health Polyt J Health Anal 2016;5: ???.

Etim LB, Chuku A, Godwin AO. Antibacterial properties of snail mucus on Bacteria isolated from patients with wound infection. Br Microbiol Res J 2015;11:1-9.

Perez WP, Dina F, Iwang Y. Effect of snail mucus (Achatina fulica) on the number of fibroblast cells in the healing of vegetable wounds. Experimental study on the skin of mice (Mus musculus). J Vet Med 2007;23:139-44.

Ibrahim KA, El-Eswed BI, Abu-Sbeih KA, Arafat TA, Al Omari MM, Darras FH, et al. Preparation of chito-oligomers by hydrolysis of chitosan in the presence of zeolite as adsorbent. Mar Drugs 2016;14:43.

Harti AS, Murharyati A, Sulisetyawati DS, Oktariani M. The effectiveness of snail mucus (Achatina fulica) and chitosan toward limfosit proliferation in vitro. Asian J Pharm Clin Res 2018;11:85-8.

Burkovski A. Cell envelope of corynebacteria: Structure and influence on pathogenicity. ISRN Microbiol 2013;2013:935736.

Wu X, Yang J, Tan G, Liu H, Liu Y, Guo Y, et al. Drug resistance characteristics of Mycobacterium tuberculosis isolates from patients with tuberculosis to 12 antituberculous drugs in China. Front Cell Infect Microbiol 2019;9:1-11.

Sutanto YS, Sutanto M, Harti AS. Prediction of Determinants of the Occurrence of Mycobacterium tuberculosis Isolate Resistance in Tuberculosis Patients. Surakarta: Research Report, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Sebelas Maret University of Surakarta; 2020.

Health Ministry Republic of Indonesia. Tuberculosis. Jakarta: Health Ministry Republic of Indonesia; 2018.

Nakagawa, Ozasa K, Yamada N, Shimouchi A, Ishikawa, Bam DS. Gender difference in delays to diagnosis and health care seeking behavior in a rural area of Nepal. Int J Tuberc Lung Dis 2001;5:24-31.

Farihatun S, Machmud PB. Determinant factors of drop out among multi drugs resting Tuberculosis patients at Jakarta Province. Int J Trop Dis 2018;7:87-92.

Suryana K. Drug provocation test as a diagnostic approach for hypersensitivity reaction to antituberculosis drugs: A report of two cases. Asian J Pharm Clin Res 2020;13:1-4.