ANTI-PARKINSON ACTIVITY OF AQUEOUS EXTRACT OF LEAVES OF MURRAYA KOENIGII AGAINST PARAQUAT-INDUCED PARKINSONISM IN WISTAR RATS

MAHESWARI REDDY B; DHANAPAL CK; LAKSHMI BVS

doi:10.22159/ajpcr.2020.v13i10.38421

Authors

MAHESWARI REDDY B Department of Pharmacology, Malla Reddy College of Pharmacy, Medchal, Telangana, India.
DHANAPAL CK Department of Pharmacy, University Institute of Pharmaceutical Technology, Faculty of Engineering and Technology, Annamalai University, Chidambaram, Tamil Nadu, India.
LAKSHMI BVS Department of Pharmacology, Malla Reddy College of Pharmacy, Medchal, Telangana, India.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i10.38421

Keywords:

Nil, Paraquat, Antioxidant parameters, Behavioral parameters, Murraya koenigii

Abstract

Objective: The current study evaluates anti-Parkinson’s activity of aqueous extracts of leaves of Murraya koenigii (MK) (AEMK) against paraquat (PQ)-induced Parkinsonism in rats.

Methods: In this study, effects of MK (100, 200, and 400 mg/kg, p.o.) were studied using in vivo behavioral parameters such as catalepsy, muscle rigidity, and locomotor activity and its effects on neurochemical parameters malondialdehyde, catalase (CAT), glutathione (GSH) reductase, GSH peroxidase, and GSH in rats.

Results: Parkinson’s disease was induced by administering PQ 10 mg/kg b.w/i.p once in a week for 4 weeks. The increased cataleptic scores were significantly (p<0.001) found to be reduced, with the AEMK in a dose-dependent manner. Chronic administration of PQ significantly induced motor dysfunction (muscle rigidity and hypolocomotion), showed a significant increase in lipid peroxidation level, and depleted the levels of GSH, CAT, and reduced GSH. Daily administration of AEMK significantly improved motor performance and also significantly attenuated oxidative damage.

Conclusion: The study proved that MK treatment significantly attenuated motor defects and also protected the brain from oxidative stress.

Downloads

Download data is not yet available.

References

Zazpe AI, Artaiz A, Innerarity E, del Olmo, Castro E. In vitro and in vivo characterization of F-97013-GD, a partial 5-HT 1A agonist with antipsychotic-and antiparkinsonian-like properties. Neuropharmacology 2006;51:129-40.

Abdel-Aal RA, Assi AA, Kostandy BB. Rivastigmine reverses aluminum-induced behavioral changes in rats. Eur J Pharmacol 2011;659:169-76.

Mccormack AL, Thiruchelvam M, Manning-Bog AB, Thiffault C, Langston JW, Cory-Slechta DA, et al. Environmental risk factors and Parkinson’s disease: Selective degeneration of nigral dopaminergic neurons caused by the herbicide PQ. Neurobiol Dis 2002;10:119-27.

Cory-Slechta DA, Thiruchelvam M, Barlow BK, Richfield EK. Developmental pesticide models of the Parkinson disease phenotype. Environ Health Perspect 2005;113:1263-70.

Bhatnagar M, Sharma D, Salvi M. Neuroprotective effects of Withania somnifera dunal.: A possible mechanism. Neurochem Res 2009;34:1975-83.

Yadav S, Gupta SP, Srivastava G, Srivastava PK, Singh MP. Role of secondary mediators in caffeine-mediated neuroprotection in maneb and paraquat-induced Parkinson’s disease phenotype in the mouse. Neurochem Res 2011;37:875-84.

Gupta S, George M, Singhal M, Sharma GN, Garg V. Leaves extract of Murraya koenigii Linn for anti-inflammatory and analgesic activity in animal models. J Adv Pharm Technol Res 2010;1:68-77.

Khuntia TK, Panda DS. Evaluation of antibacterial, antifungal and anthelmintic activity of Murraya koenigii Spreng. Int J Pharm Sci 2011;2:105-10.

Dixit A, Srivastava G, Verma, D, Mishra M, Singh PK, Prakash O, et al. Minocycline, levodopa and MnTMPyP induced changes in the mitochondrial proteome profile of MPTP and maneb and paraquat mice models of Parkinson’s disease. Biochim Biophys Acta 2013;1832:1227-40.

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;17:214-26.

Mahadevan MV, Ramaswamy RS, Banumathi V. Mimosa pudica exerts neuroprotection against mpp+induced neurotoxicity in shsy5y cell lines-an in vitro model of anti-parkinsonism. Int J Pharm Pharm Sci 2016;9:21-6.

Suryakanta P, Abhisek P, Pratap KS. Neuroprotective effect of quercetin in neurotoxicity induced rats: Role of neuroinflammation in neurodegeneration. Asian J Pharm Clin Res 2014;7:152-6.

Srimathi PK, Vijayalakshmi K, Selvaraj R. Behavioral studies of Wistar rats in rotenone induced model of Parkinson’s disease. Int J Pharm Pharm Sci 2017;11:159-64.