MICROBIAL ENZYMATIC REDUCTION OF IRON NANOPARTICLES FOR THE CONTROL OF HUMAN PATHOGENS, STAPHYLOCOCCUS AUREUS, AND SALMONELLA TYPHI
DOI:
https://doi.org/10.22159/ajpcr.2020.v13i12.38607Keywords:
Microbial synthesis, Nanoparticles characterization, Antibacterial activity, Zone inhibition, Staphylococcus aureus, Salmonella typhiAbstract
Objective: The objective of this study was to synthesis, characterize, and evaluation of antimicrobial potential of iron nanoparticles (Fe NPs) using Serratia marcescens.
Methods: Fe NPs were fabricated by microbial enzyme using ferric chloride as an agent of reduction and stabilization. Fe NPs formation and their elemental nature were confirmed by ultraviolet (UV)-absorption spectroscopy and energy dispersive X-ray spectroscopy, respectively. The morphology of Fe NPs was characterized by a scanning electron microscope (SEM). Functional groups of biomolecules associated with Fe NPs were inferred from characteristic Fourier transform infrared (FTIR) spectroscopy peaks. The antibacterial activity is determined by the disc diffusion method.
Results: Synthesized Fe NPs exhibited characteristic UV-absorption spectrum peaks at 263nm. FTIR spectroscopy peaks of Fe NPs, 3411.66, 1629.16, 1039.63, and 601.90 cm−1 corresponds to carbonyl, disulfides, and ethers groups. SEM study demonstrated that the average size was from 200nm with interparticle distances. The crystalline nature of Fe NPs was confirmed from the X-ray diffraction peaks analysis. The intense diffraction peaks due to Fe NPs at 16.32, 22.56, 35.54, 41.08, 52.36, 61.42, 66.42, 78.1, and 85.08. Corresponding to the 110, 150, 200, 430, 550, and 950 facets of the face-centered cubic crystal structure conformed to the Joint Committee on Powder Diffraction Standards: 89-3722 of iron. Antimicrobial activity of Fe NPs against tested Gram-positive and negative bacterial strains showed significant inhibitory zones.
Conclusion: The inhibitory zones obtained in the present study reveal that the Fe NPs can act as a good antibacterial agent.
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Abdeen S, Isaac RS, Geo S, Sornalekshmi S, Rose A, Praseetha PK. Evaluation of antimicrobial activity of biosynthesized iron and silver nanoparticles using the fungi Fusarium oxysporum and Actinomycetes sp. on human pathogens. Nano Biomed Eng 2013;5:39-45.
Blakemore RP. Magnetotactic bacteria. Annu Rev Microbiol 1983;36:217-38.
Iravani S. Bacteria in nanoparticle synthesis: Current status and future prospects. Int Sch Res Notices 2014;2014:359316.
Ravindra NM. Magnetic iron oxide nanoparticles: Synthesis and applications. Bioinspir Biomim Nanobiomater 2012;1:229-44.
Wertheim HF, Melles DC, Vos MC, Van Leeuwen W, Van Belkum A, Verbrugh HA, et al. The role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis 2005;5:751-62.
Tong SY, Davis JS, Eichenberger E, Holland TL, Fowler VG. Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev 2015;28:603-61.
Wain J, Hendriksen RS, Mikoleit ML, Keddy KH, Ochiai RL. Typhoid fever. Lancet 2015;385:1136-45.
Prema P, Thangapandiyan S. In-vitro antibacterial activity of gold nanoparticles capped with polysaccharide stabilizing agents. Int J Pharm Pharm Sci 2013;5:310-4.
Dizaj SM, Lotfipour F, Barzegar-Jalali M, Zarrintan MH, Adibkia K. Antimicrobial activity of the metals and metal oxide nanoparticles. Mater Sci Eng C Mater Biol Appl 2014;44:278-84.
Fair RJ, Tor Y. Antibiotics and bacterial resistance in the 21st century. Perspect Med Chem 2014;6:25-64.
Pelgrift RY, Friedman AJ. Nanotechnology as a therapeutic tool to combat microbial resistance. Adv Drug Deliv Rev 2013;65:1803-15.
Mahapatra O, Ramaswamy S, Nune SV, Yadavalli T, Gopalakrishnan C. Corn flake-like morphology of iron nanoparticles and its antibacterial property. J Gen Appl Microbiol 2011;57:59-62.
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