ANTI-MELANOMA BIO-EFFICACY OF THE PLANT MADHUCA LONGIFOLIA AND ITS ENHANCEMENT USING BIOACTIVE PRINCIPLE LOADED GOLD NANOPARTICLE

SAURABH YADAV; MUKTI SHARMA; NARAYANAN GANESH; SHALINI SRIVASTAVA; M. M. SRIVASTAVA

doi:10.22159/ajpcr.2020.v13i10.38832

Authors

SAURABH YADAV Department of Chemistry, Dayalbagh Educational Institute, Dayalbagh, Agra, India.
MUKTI SHARMA Department of Chemistry, Dayalbagh Educational Institute, Dayalbagh, Agra, India.
NARAYANAN GANESH Jawaharlal Nehru Cancer Hospital and Research Center, Bhopal, India.
SHALINI SRIVASTAVA Department of Chemistry, Dayalbagh Educational Institute, Dayalbagh, Agra, India.
M. M. SRIVASTAVA Department of Chemistry, Dayalbagh Educational Institute, Dayalbagh, Agra, India.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i10.38832

Keywords:

Madhuca longifolia, Flavonoid, Gold nanoparticles, Enhanced bio-efficacy

Abstract

Objective: Unexplored in-vivo anti-melanoma bio-efficacy of the plant Madhuca longifolia (bark) has been carried out against C57BL/6 mice.

Methods: Optimized experimental conditions of phytofabrication of gold nanoparticles were as follows: flavonoid content (1 ml, 0.5 mg/ml), sodium tetrachloroaurate dihydrate solution (2 ml, 1 mM), and sonication (15 min, 20 KHz) at pH 4. The optical properties; ultraviolet-visible spectrophotometer (UV-Vis), particles size and zeta potential (Zetasizer), miller indices; X-ray diffraction (XRD), morphology; field emission-scanning electron microscope (FE-SEM), particle size; high resolution-transmission electron microscopy (HR-TEM), surface roughness; atomic force microscopy (AFM) and elemental composition; and energy dispersive X-rays (EDX) of flavonoid loaded gold nanoparticles. In-vivo anti-melanoma bio-efficacy has been carried out against C57BL mice. Radioisotopic, hematological, and histopathological studies were carried out using standard procedures.

Results: Redox potential of the total flavonoid extracted from the bark of the plant (Madhuca longifolia) has been used for the fabrication of flavonoid loaded gold nanoparticles (F@AuNp) and confirmed for the first time their significant anti-melanoma bio-efficacy. The finding is supported by hematological and histopathological studies carried out in the organs (liver, kidney, and intestine) of C57BL mice. The significant enhancement in phytofabricated F@AuNp compared to native bark extract of the plant has been assigned to enhanced stay period and nanosizing, biocompatibility, nontoxic nature, and enhanced beneficial payload to the cancerous cells.

Conclusion: Such phytofabricated gold nanoparticles possess an admirable prospect for the expansion of herbal nanomedicine for anti-melanoma bio-efficacy.

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