DELONIX REGIA BOJ. EX. HOOK RAFFIN; FAMILY: FABACEAE, BARK METHANOL EXTRACT PHYTOCHEMICAL PROFILE AND POTENTIAL THERAPEUTIC EVALUATION

ARPITHA SHIVAMALLU; SHAILASREE SEKHAR

doi:10.22159/ajpcr.2020.v13i10.38868

Authors

ARPITHA SHIVAMALLU Scholar, Institution of Excellence, Vijnana Bhavana, University of Mysore, Mysuru.
SHAILASREE SEKHAR Scientist, Institution of Excellence, Vijnana Bhavana, University of Mysore, Mysuru.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i10.38868

Keywords:

LC analysis in negative mode, Antioxidant, 15-LOX, COX-2, Biofilm by Confocal laser scanning microscopy, MCF-7 cells cytotoxicity

Abstract

Objectives: The aim of this study was to evaluate the antioxidant, anti-inflammatory, and anti-cancer potencies of the Delonix regia bark, a first of its kind.

Methods: The bark was extracted sequentially in Soxhlet apparatus with hexane, chloroform, and methanol in the increasing order of polarity. These extracts were subjected to find its antioxidant activity and total phenol content. Antibacterial activity against human pathogenic bacteria was tested. The anti-inflammatory properties were elucidated by its capacity to inhibit 15-lipoxygenase (LOX) and human cyclooxygenase (COX)-2. Cell cytotoxic capacity was evaluated against MCF-7 cells breast cancer cell lines.

Results: Liquid chromatography (LC)-Mass Spectroscopy (MS) fingerprint of the methanol extract identified a total of 14 polyphenols, of which five were structurally characterized based on their mass-charge ratio [M-H]− peak, UV-vis absorption in comparison to published data. Antibacterial activity by disk diffusion inhibited human pathogenic bacteria. Bacterial biofilm inhibition capacity of extract (750 mg) imaged by confocal laser scanning microscopy revealed loss of microcolonies. Extract when tested for 15-LOX inhibition exhibited IC50 values of 94.5 ± 1.23 mg.mL−1 by enzyme kinetics studies using spectrophotometric techniques. Similarly, it could inhibit COX-2 enzyme at relatively lower concentrations (32.18 ± 1.91 mg.mL−1). Further, it quenched free radicals produced by Fentons’ reagent studied by DNS-nicking assay indicating its strong antioxidant property with the capacity to protect DNA. In vitro cytotoxicity was evaluated by 3-(4,5-dimethylthylthiazol-2-yl)-2,5-diphynyl tetrazolium bromide assay and apoptosis induced in MCF-7 cells was assessed morphologically.

Conclusion: Our data suggest that D. regia bark methanol extract exerts its therapeutic activity for further pharmaceutical evaluations. Further studies are necessary to determine the mechanisms of these pharmacological properties.

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Author Biography

ARPITHA SHIVAMALLU, Scholar, Institution of Excellence, Vijnana Bhavana, University of Mysore, Mysuru.

Researcher

References

Modi A, Mishra V, Bhatt A, Jain A, Mansoori MH, Gurnany E, et al. Delonix regia: Historic perspectives and modern phytochemical and pharmacological researches. Chin J Nat Med 2016;4:31-9.

Sharma S, Arora S. Phytochemicals and pharmaceutical potential of Delonix regia (Bojer Ex Hook) Raf a review. Int J Pharm Pharm Sci 2015;7:17-9.

Khare CP. Indian Medicinal Plants, An Illustrated Dictionary. 1st ed. New Delhi: Springer; 2007. p. 812.

Parke DV, Lewis DF. Safety aspects of food preservatives. Food Addit Contam 1992;9:561-7.

Davidson PM, Taylor MT. Chemical preservatives and natural antimicrobial compounds. In: Doyle M, Beuchat L, editors. Food Microbiology: Fundamentals and Frontiers. Washington, DC: ASM; 2007. p. 713-5.

Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J Agric Food Chem 1998;46:4113-17.

Sultanova N, Makhmoor T, Abilov ZA, Parween Z, Omurkamzinava VB, ur-Rahman A, Choudhary IM. Antioxidant and antimicrobial activities of Tamarix ramosissima. J Ethnopharmacol 2001;78:201-5.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical. Free Radic Biol Med 1998;26:1231-37.

Klancnik A, Piskernik S, Jeršek B, Možina SS. Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. J Microbiol Methods 2010;81:121-6.

Zhang W, Wang Y, Tian RM, Bougouffa S, Yang B, Cao HL, et al. Species sorting during biofilm assembly by artificial substrates deployed in a cold seep system. Sci Rep 2015;4:6647-54.

Rackova L, Oblozinsky M, Kostalova D, Kettmann V, Bezakova L. Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids. J Inflammation 2007;4:15-1.

Sekhar S, Sampathkumara KK, Niranjana SR, Prakash HS. Bioactive potential of medicinal plants from Western Ghats region, India. J Herbs Spices Med Plants 2014;20:221-4.

Choi JY, Desta KT, Lee SJ, Kim YH, Shin SC, Kim GS, et al. LC-MS/MS profiling of polyphenol-enriched leaf, stem and root extracts of Korean Humulus japonicus Siebold and Zucc and determination of their antioxidant effects. Biomed Chromatogr 2018;32:1-8.

Lee JC, Kim HR, Kim J, Jang YS. Antioxidant activity of ethanol extract of the stem of Opuntia ficus-indica var. Saboten. J Agric Food Chem 2002;50:1619-24.

Peterson J, Dwyer J. Flavonoids: Dietary occurrence and biochemical activity. Nutr Res 1998;18:1995-98.

Semaming Y, Pannengpetch P, Chattipakorn SC, Chattipakorn N. Pharmacological properties of protocatechuic acid and its potential roles as complementary medicine. Evid Based Complement Alternat Med 2015;2015:593902.

Jafari N, Zargar SJ, Delnavazi MR, Yassa N. Cell cycle arrest and apoptosis induction of phloroacetophenone glycosides and caffeoylquinic acid derivatives in gastric adenocarcinoma (AGS) cells. Anticancer Agents Med Chem 2008;18:610-6.

Lin Y, Shi R, Wang X, Shen HM. Luteolin, a flavonoid with potentials for cancer prevention and therapy. Curr Cancer Drug Targets 2008;8:634-6.

Brito A, Ramirez JE, Areche C, Sepúlveda B, Simirgiotis MJ. HPLC-UV-MS profiles of phenolic compounds and antioxidant activity of fruits from three citrus species consumed in Northern Chile. Molecules 2014;19:17400-21.

Spínola V, Pinto J, Castilho PC. Identification and quantification of phenolic compounds of selected fruits from Madeira Island by HPLC-DAD-ESI-MSn and screening for their antioxidant activity. Food Chem 2015;173:14-30.

Lin LZ, Harnly JM. Identification of the phenolic components of chrysanthemum flower (Chrysanthemum morifolium Ramat). Food Chem 2010;120:319-6.

Chaira T, Barman T, Raj VS. In vitro ADME, preclinical pharmacokinetics and prediction of human pharmacokinetics of RBx14255, a novel ketolide with pharmacodynamics against multidrug-resistant bacterial pathogens. Int J Pharm Pharm Sci 2020;23:206-9.

Ali S, Sheikh K, Amber R. Review on antibacterial activity of Himalayan medicinal plants traditionally used to treat pneumonia and tuberculosis. J Pharm Pharmacol 2019;71:1599-25.

Jose BS, Martin R. Simarouba glauca bark extract mediated synthesis and characterization of iron oxide and silver nano particles and their antibacterial, cytotoxic and photocatalytic activity. IJPPS 2020;12:9-2.

Kokila NR, Mahesh B, Mruthunjaya K. Exploration of bioactive components of Thunbergia coccinea, its pharmacognostic, antioxidant, GCMS and antihyperglycemic studies. IJPPS 2020;12:45-4.

Jain HK, Swami PN, Gujar KN. Formulation and evaluation of an antimicrobial mucoadhesive dental gel of Azadirachta indica and Glycyrrhiza glabra. IJAP 2019;11:176-84.