PHYTOCHEMICAL AND ANTIBACTERIAL ACTIVITY OF HEXANE AND ETHYL ACETATE EXTRACT OF KNOXIA SUMATRENSIS (RETZ.) DC.
DOI:
https://doi.org/10.22159/ajpcr.2021.v14i7.39750Keywords:
Phytochemical, Ethyl acetate, Disk diffusion method, Staphylococcus aureusAbstract
Objective: The study was carried out the phytochemical and antibacterial activity of Knoxia sumatrensis (Retz.) Dc. using hexane and ethyl acetate extracts.
Methods: The phytochemical screening was extracted from hexane and ethyl acetate solvent and its screening was analyzed by standard procedure. GC-MS technique was analyzed in ethyl acetate extract to identify the components present in the extract. The hexane and ethyl acetate extract of the plant was tested for antibacterial activity against human pathogenic bacteria using disk diffusion method.
Results: The phytochemical screening was revealed the presence of phenols, saponins, flavonoids, cardiac glycosides, steroids, and tannins. The GC-MS results showed that the presence of seven bioactive compounds in ethyl acetate extract. The major compounds were identified such as N-Hexadecanoic Acid (9.336), 2-Piperidinone, N-[4-Bromo-N-Butyl] - (37. 883). The ethyl acetate extract showed good antibacterial activity. The maximum zone of inhibition was noticed in S. aureus (8.25 mm) using ethyl acetate extracts at 50 μg.
Conclusion: Thus, this study the information regarding the phytochemical constituents present in the both extract. Hence, it can be used for further therapeutic applications in the near future study.
Downloads
References
Awaad AS, Zain ME, Al Othman MR, Al-Dosary SK. Phytochemical analysis and antimicrobial activity of medicinal plants against pathogenic microorganisms. Life Sci J 2014;11:350-4.
Abidullah S. Phytochemistry and antibacterial activities of some selected plants of war affected area of Bajaur agency, Pakistan. J Pharmacogn Phytochem 2018;7:415-22.
Babel S, Guptha R, Sachihar L. Phytochemical analysis of plant resourses having antimicrobial properties obtained from Aravalli hills of Rajasthan, India. Int J Curr Microb App Sci 2018;7:1121-5. DOI: https://doi.org/10.20546/ijcmas.2018.703.133
World Health Organization. Antimicrobial Resistance. Geneva: World Health Organization; 2017. Available from: http://www.who.int/ mediacentre/factsheets/fs194/en. [Last accessed on 2017 Apr 24].
World Health Organization. Antimicrobial Resistance from WHO. World Health Organization. WHO Model Prescribing Information: Drugs Used in Bacterial Infections. Available from: http://www.apps; http://www.who.int/mediacentre/factsheets/fs194/en. [Last accessed on 2017 Apr 24].
Mabberley DJ. Mabberley’s Plant-book: A Portable Dictionary of Plants, Their Classification and Uses. 4th ed. Cambridge: Cambridge University Press; 2017. DOI: https://doi.org/10.1017/9781316335581
Vivek K, Rao R. Some interesting indigenous beverages among the tribals of Central India. Indian J Tradit Know 2007;6:141-3.
Harborne JB. A Guide to Modern Techniques of Plant Analysis. London, United Kingdom: Chapman and Hall; 1973.
Bauer AW. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 1996;45:149-58.
Raju R, Deepa A, Vanathi P, Vidhya D. Screening for phytochemicals and FTIR analysis of Myristica dactyloids fruit extracts. Int J Pharm Pharm Sci 2017;9:315-8. DOI: https://doi.org/10.22159/ijpps.2017v9i1.11053
Yogeshwari C, Kumudha P. Phytochemical evaluation of Tiliacora racemosa colebr using gas chromatography-mass spectrometry. Asian J Pharm Clin Res 2018;11:350-3. DOI: https://doi.org/10.22159/ajpcr.2018.v11i2.23361
Renji Nair R, Gangaprasad A. GC-MS analysis of methanolic stem extract of Gynochthodes Ridsdalei, Razafim and B. Bremer, an endemic, endangered medicinal plant of Southern Western Ghats. Int J Curr Pharm Res 2017;9:98-101. DOI: https://doi.org/10.22159/ijcpr.2017.v9i3.19665
Devi JA, Muthu AK. Gas chromatography-mass spectrometry analysis of phytocomponents in the ethanolic extract from whole plant of lactuca runcinata dc. Gas 2015;8:202-6.
Tyagi T, Agarwal M. Phytochemical screening and GC-MS analysis of bioactive constituents in the ethanolic extract of Pistia stratiotes L. and Eichhornia crassipes (Mart.) solms. J Pharmacogn Phytochem 2017;6:95-206. DOI: https://doi.org/10.22159/ijcpr.2017.v9i3.19970
Arora S, Meena S. GC-MS profiling of Ceropegia bulbosa Roxb. var. bulbosa, an endangered plant from Thar Desert, Rajasthan. J Pharm Innov 2017;6:568-73. DOI: https://doi.org/10.7897/2230-8407.086100
Al-Salman HN. Antimicrobial activity of the compound 2-piperidinone, n-[4-bromo-n-butyl]-extracted from pomegranate peels. Asian J Pharm 2019;13:46-53.
Sugiyarto, Novalina D, Susilowati A, Sasongko H. Antibacterial activity of ethyl acetate and n-hexane fractions of Carica pubescens rind and seeds. In: AIP Conference Proceedings. Vol. 2019. United States: AIP Publishing LLC; 2018. p. 050005. DOI: https://doi.org/10.1063/1.5061898
Anita A, Punitha D, Selvaraj D. Screening of marine gastropods for potential antibacterial substances against pathogenic bacteria. Int J Zool Appl Biosci 2020;5:180-4.
Chimahali J, Jebamalar A, Duraikannu G, Thirumal S. Phytochemical analysis and evaluation of antimicrobial activity in the whole plant extracts of Gloriosa superba. Phanel 2019;12:245-9. DOI: https://doi.org/10.22159/ajpcr.2019.v12i6.33059
Bindu AR, Rosemary J, Akhila S. Antimicrobial activity screening of M. minuta extracts. Int J Pharm Pharm Sci 2014;6:581-3.
Soni A, Dahiya P. Screening of phytochemicals and antimicrobial potential of extracts of Vetiver zizanoides and Phragmites karka against clinical isolates. Int J App Pharm 2015;7:22-4.
Published
How to Cite
Issue
Section
Copyright (c) 2020 K. SELVAM
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.