STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF FIMASARTAN BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY IN BULK AND PHARMACEUTICAL DOSAGE FORM

SRUTHI A; UTTAM PRASAD PANIGRAHY

doi:10.22159/ajpcr.2021.v14i2.39919

Authors

SRUTHI A Department of Pharmaceutical Analysis, CMR College of Pharmacy, Hyderabad, Telangana, India.
UTTAM PRASAD PANIGRAHY Department of Pharmaceutical Analysis, CMR College of Pharmacy, Hyderabad, Telangana, India.

DOI:

https://doi.org/10.22159/ajpcr.2021.v14i2.39919

Keywords:

Fimasartan, Reverse-phase high-performance liquid chromatography,, C18 column

Abstract

Objective: A rapid, sensitive and specific reverse phase High performance liquid Chromatography (RP-HPLC) method was developed for the estimation of Fimasartan in bulk and pharmaceutical dosage form.

Method: The RP-HPLC analysis was performed isocratically on a Primacel C18 column (150 mm × 4.6 mm internal diameter, 5 μm particle size) using mobile phase of composition Acetonitrile and 0.1% orthophosphoric Acid in 80:20, v/v proportions with a flow rate of 0.8 ml/min.

Results: The analyte was monitered with UV-detector at 265 nm. In the developed method Fimasartan elutes at a typical retention time of 2.4 min. The proposed method is having linearity in the concentration ranging from 5-30 μg/ml of Fimasartan.

Conclusion: : The method was statistically validated and had been applied to analysis of the drug in bulk and pharmaceutical dosage form.

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