ANTIBIOTIC SUSCEPTIBILITY PATTERN OF ESCHERICHIA COLI ISOLATED FROM CHILDREN WITH URINARY TRACT INFECTION

YOGESH OLI; GANESH BHANDARI; UPASHANA BHANDARI; SUNITA BISTA; AMRIT KUMAR BHATTARAI; ARCHANA KATUWAL; NETRA LAL BHANDARI

doi:10.22159/ajpcr.2021.v14i2.40128

Authors

YOGESH OLI Department of Microbiology, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal.
GANESH BHANDARI Department of Chemistry, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal.
UPASHANA BHANDARI Department of Chemistry, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal.
SUNITA BISTA Department of Chemistry, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal.
AMRIT KUMAR BHATTARAI Department of Microbiology, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal.
ARCHANA KATUWAL Department of Microbiology, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal.
NETRA LAL BHANDARI Department of Chemistry, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal.

DOI:

https://doi.org/10.22159/ajpcr.2021.v14i2.40128

Keywords:

Antimicrobial resistance, Ceftriaxone, Multidrug-resistant, Staphylococcus aureu

Abstract

Objective: This work aimed to detect the antibiotic susceptibility pattern of Escherichia coli isolated from children, as it is the most predominant pathogen of urinary tract infection (UTI).

Methods: About 530 urine samples were collected and tested using the modified Kirby–Bauer disk diffusion method to find the susceptibility pattern of isolated bacteria.

Results: Out of a total of 530 samples, 114 (21.50%) showed significant growth. A total of 8 different types of bacteria were isolated from the growth of positive samples. Among the isolates, E. coli 66 (57.8%) was found to be the most predominant organism followed by Klebsiella pneumoniae 18(15.8%), Proteus spp. 10 (8.8%), Staphylococcus aureus 8 (7.0%), Acinetobacter spp. 4 (3.5%), CoNS 4 (3.5%), Enterobacter spp. 2 (1.8%), and Pseudomonas aeruginosa 2 (1.8%). In the present study, out of 66 E. coli, 37 (56.1%) were multidrug-resistant strain. E. coli showed 94.0% resistance to ceftriaxone followed by ceftazidime 86.5% and cefotaxime 70.3%. Imipenem (91.9%) followed by amikacin (89.2%) seems to be the effective drug against UTI causing E. coli in children.

Conclusion: Multidrug resistance may possess difficulties with the choice of therapeutic options for the treatment of severe infections.

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