EVALUATION AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR THE USE OF BERBERINE FROM COPTIS TEETA BARK AS ANTI-CANCER PHYTOCHEMICAL
DOI:
https://doi.org/10.22159/ajpcr.2021.v14i4.40400Keywords:
HPLC, Berberine, Coptis teeta, ValidationAbstract
Objective: A new method of analysis using high-performance liquid reverse chromatography (HPLC) to calculate berberine in Coptis teeta bark was created in addition to the validation research.
Methods: Berberine was analyzed with potassium hydrogen phosphate and acetonitrile using the SunFire C18 column at a flow rate of 1.0 ml/min; 5μ (4.6 × 250 mm) was detected at 347 nm.
Results: For precision, selectivity, and linearity, the technique was tested. The quantification of berberine did not conflict with the existence of other chemical components. Between concentrations of 60 and 180 ppm, the calibration curve was linear. The precision ranged from 9.0% to 102.0% and 2% was the relative standard deviation.
Conclusion: Finally, this method of HPLC enhancement is simple, effective and has been used to classify and quantify berberine successfully. This can be useful for the study of different berberine-containing plant extracts and can also be used to measure berberine from formulation to herbal formulation.
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