COMPARATIVE IN VITRO ANTIOXIDANT ACTIVITY OF ETHYL ACETATE AND ETHANOL EXTRACTS OF CALLICARPA MACROPHYLLA

GANESH N SHARMA; HARJINDER KAUR; BIRENDRA SHRIVASTAVA; SATISH CHANDER ARORA

doi:10.22159/ajpcr.2021.v14i5.41014

Authors

GANESH N SHARMA Department of Pharmacognosy, School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, India.
HARJINDER KAUR Department of Pharmacognosy, School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, India.
BIRENDRA SHRIVASTAVA Department of Pharmacognosy, School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, India.
SATISH CHANDER ARORA Department of Pharmaceutical Sciences, RKSD College of Pharmacy, Kaithal, Haryana, India.

DOI:

https://doi.org/10.22159/ajpcr.2021.v14i5.41014

Keywords:

Callicarpa macrophylla, Antioxidant activity, Total flavonoid content, Phenol content, Physical evaluation

Abstract

Objective: The objective of the study was to evaluate and compare the antioxidant activity of (EA and E) ethyl acetate and ethanol extracts of (CM) Callicarpa macrophylla.

Methods: The physiochemical parameters were assessed according to guidelines given by the world health organization. The total content of phenols and flavonoids was assessed by Folin–Ciocalteu and aluminum chloride methods. In vitro, antioxidant activity was screened by (DPPH) 1, 1-diphenyl-2- picrylhydrazyl and(H2O2) hydrogen peroxide scavenging and reducing power assay.

Results: The physicochemical parameters fulfilled the standards of WHO guidelines. Total phenol and flavonoid content were more in ethanol extract as compared to ethyl acetate extract of CM. The antioxidant activity of ethanol extract was further high as compared to ethyl acetate extract of Callicarpa macrophylla. The IC50 of Callicarpa macrophylla ethanol extract was less than the ethyl acetate extract. So, more antioxidant activity of ethanol extract compared to ethyl acetate extract of CM.

Conclusion: Overall, both the extracts showed antioxidant activity and can be used further for diseases that can be managed using antioxidants. Ethanol extract possessed significant antioxidant effects than the ethyl acetate extract.

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