CHEMICAL EXAMINATION AND IN VITRO ANTIFUGAL CHRACTERIZATION OF ESSENTIAL OIL FROM AERIAL PARTS OF SWERTIA PETIOLATA D. DON

SANJAY KUMAR; PAWAN KUMAR; MOHAMMAD SUHEL JAVED; SACHIN GUPTA

doi:10.22159/ajpcr.2022.v15i6.41466

Authors

SANJAY KUMAR Deparatment of Chemistry, D. S. B. Campus, Kumaun University, Nainital, India. https://orcid.org/0000-0001-9930-8846
PAWAN KUMAR Deparatment of Chemistry, D. S. B. Campus, Kumaun University, Nainital, India.
MOHAMMAD SUHEL JAVED Deparatment of Chemistry, D. S. B. Campus, Kumaun University, Nainital, India.
SACHIN GUPTA Division of Plant Pathology, FAO-Chatha, Sher-e-Kashmir University of Agricultural Sciences and Technology-Jammu, Jammu and Kashmir, India.

DOI:

https://doi.org/10.22159/ajpcr.2022.v15i6.41466

Keywords:

Essential oil, Swertia petiolata, Phytochemical, Antifungal., Strain

Abstract

Objective: The aim of this research work was to evaluate the important phytochemical and their antifungal activity against crops pathogens from essential of Swertia petiolata.

Methods: The phytochemical composition and antifungal activity of essential oil from aerial parts of Swertia petiolata D. Don obtained from hydro-distillation and analyzed by GC/GC-MS analysis in relation with their Kavot indices and mass spectra and their antifungal activity by disk diffusion method.

Results: The oil is rich in monoterpenoids and oxygenated sesquiterpenoids. A total of 39 chemical constituents were identified representing 87.50% and major chemical constituents were identified as n-tetradecanal (15.16%), isopropylcyclohexene (13.58%), trimethylsilylpalmitate (12.50%), longipianol (7.76%), n-eocosane (7.46%), z-patchenal (4.40%), guaiadienal (4.07%), and heptadecanal (3.86%) whereas oxygenated monoterpenoids were minor constituents. The antifungal activity was studied by disk diffusion method with 57.63±0.10% and 44.34±0.13% mycelial growth inhibition. The oil was active against Bipolaris maydis and Rhizoctonia solani at concentration of 2.5μg/μL while, Fusarium oxysporum and Alternaria alternata were least active for this oil. The minimum inhibitory concentration and IC50 showed a range from 1.2 μg/μL to 1.8 μg/μL as compared with standard fungicides (Amphotericin and Clotrimazole) with IC50 values ranging from 0.5 μg/μL to 0.9 μg/μL and 0.5 μg/μL to 1.5 μg/μL.

Conclusions: The essential oil dominated by n-tetradecanal (15.16%), isopropylcyclohexene (13.58%), trimethylsilylpalmitate (12.50%), longipianol (7.76%), n-eocosane (7.46%), z-patchenal (4.40%), and guaiadienal (4.07%) as the major components from aerial parts which showed a marked antifungal activity against Bipolaris maydis and Rhizoctonia solani.

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