CHEMICAL EXAMINATION AND IN VITRO ANTIFUGAL CHRACTERIZATION OF ESSENTIAL OIL FROM AERIAL PARTS OF SWERTIA PETIOLATA D. DON
DOI:
https://doi.org/10.22159/ajpcr.2022.v15i6.41466Keywords:
Essential oil, Swertia petiolata, Phytochemical, Antifungal., StrainAbstract
Objective: The aim of this research work was to evaluate the important phytochemical and their antifungal activity against crops pathogens from essential of Swertia petiolata.
Methods: The phytochemical composition and antifungal activity of essential oil from aerial parts of Swertia petiolata D. Don obtained from hydro-distillation and analyzed by GC/GC-MS analysis in relation with their Kavot indices and mass spectra and their antifungal activity by disk diffusion method.
Results: The oil is rich in monoterpenoids and oxygenated sesquiterpenoids. A total of 39 chemical constituents were identified representing 87.50% and major chemical constituents were identified as n-tetradecanal (15.16%), isopropylcyclohexene (13.58%), trimethylsilylpalmitate (12.50%), longipianol (7.76%), n-eocosane (7.46%), z-patchenal (4.40%), guaiadienal (4.07%), and heptadecanal (3.86%) whereas oxygenated monoterpenoids were minor constituents. The antifungal activity was studied by disk diffusion method with 57.63±0.10% and 44.34±0.13% mycelial growth inhibition. The oil was active against Bipolaris maydis and Rhizoctonia solani at concentration of 2.5μg/μL while, Fusarium oxysporum and Alternaria alternata were least active for this oil. The minimum inhibitory concentration and IC50 showed a range from 1.2 μg/μL to 1.8 μg/μL as compared with standard fungicides (Amphotericin and Clotrimazole) with IC50 values ranging from 0.5 μg/μL to 0.9 μg/μL and 0.5 μg/μL to 1.5 μg/μL.
Conclusions: The essential oil dominated by n-tetradecanal (15.16%), isopropylcyclohexene (13.58%), trimethylsilylpalmitate (12.50%), longipianol (7.76%), n-eocosane (7.46%), z-patchenal (4.40%), and guaiadienal (4.07%) as the major components from aerial parts which showed a marked antifungal activity against Bipolaris maydis and Rhizoctonia solani.
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References
Bhan S, Kumar R, Kalla AK, Dhar KL. Triterpenoids from Swertia petiolata. Phytochemistry 1988;27:539-42. doi: 10.1016/0031- 9422(88)83137-6
Bhargawa S, Rao PS, Bhargawa P, Shukla SS. Antipyretic potential of Swertia chirata Buch Ham. Root extract. Sci Pharm 2009;77:617-23.
Chen Y, Huang B, He J, Han L, Zang Y, Wang Y. In-vitro and in-vivo antioxidant effect of the ethanolic extract of Swertia chirayita. J Ethanopharmacol 2011;136:309-15.
Feng W, Zheng X. Essential oils to control Alternaria alternata in-vitro and in-vivo. Food Control 2007;18:1126-30. doi: 10.1016/j. foodcont.2006.05.017
Gairola S, Sharma J, Bedi YS. Across-cultural analysis of Jammu, Kashmir and Ladakh (India) medicinal plants use. J Ethanopharmacol 2014;155:925-86. doi: 10.1016/j.jep.2014.06.029
Ghosal S, Sharma PV, Choudhary RK, Bhattacharya SK. Chemical constituents from gentianceae XIV: Tetraoxygenated and pentaoxygenated xanthones of Swertia purpurascens wall. J Pharm Sci 1975;64:80-3.
Ghosal S, Sharma PV, Chaudhuri RK. Tetra and pent oxygenated xanthones of Swertia lawaii. Phytochemistry 1975;14:1393-6. doi: 10.1016/S0031-9422(00)98635-7
Ghosal S, Sharma PV, Chaudhuri RK. Xanthones of Swertia bimaculata. Phytochemistry 1975;14:2671-5. doi: 10.1016/0031- 9422(75)85248-4
Sharma N, Varshney VK, Kala P, Bisht B, Sharma M. Antioxidant capacity and total phenolic contents of Swertia Chirata (Roxb ex Fleming) H. Karstt. in Uttarakhand. Int J Sci Rev Res 2013;23:251-61.
Sharma PV. Alkaloids of Swertia chirata. Indian J Pharm Sci 1982;44:36-52.
Srivastava S, Singh RP. Antifungal activity of the essential oil of Murraya koenigii (L). Spreng Indian Perfumer 2001;45:49-51.
Wang H, Yuan X, Huang H, Zhang B, Cao C, Zhao HP. Chemical constituents from Swertia mussotii franch. (Gentianaceae). Nat Prod Res 2017;31:1704-8. doi: 10.1080/14786419.2017.1286480, PMID 28278647
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