• SANJAY NIPANIKAR Department of Ari Healthcare Private Limited, World Trade Center, Pune, Maharashtra, India.
  • CHITLANGE SS Department of D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pune, Maharashtra, India.




Ariflex tablet, Aceclofenac and diclofenac tablet, Analgesic, Acetic acid induced writhing model


Objective: The present study was conducted to evaluate analgesic activity of Ariflex Tablet which is a polyherbal formulation conceptualized and developed by Ari Healthcare Private in comparison to Aceclofenac and Diclofenac Tablet.

Methods: Albino mice of either sex weighing 20–25 g were taken and divided into four groups with six animals in each group. Group 1 (Controlled Group) animals were starved overnight. Group 2 animals were orally administered with Diclofenac Tablet as Standard drug. Group 3 animals were orally administered with Aceclofenac Tablet as Standard drug and Group 4 Animals were orally administered with Ariflex Tablet. The test and standard drugs were orally administered with feeding needle after 1 h of injecting 1% acetic acid intraperitoneally in volume of 0.1 ml/10 g body weight. Writhing episodes were recorded for 30 min by counting the stretching.

Results: All the tested formulations possess analgesic activity in acetic acid induced writhing model. Aceclofenac possesses strong analgesic activity compared to other formulations tested. In Ariflex Tablet Group, the number of writhes was 120.6±41.4. If compared to control group, the number of writhes was significantly less suggesting analgesic activity of Ariflex Tablet. Analgesic activity of Ariflex Tablet was close to that of Diclofenac Sodium.

Conclusion: It can be concluded that Ariflex Tablet possesses significant analgesic activity. Ariflex Tablet can be used in the management of Osteoarthritis, Rheumatoid arthritis, Gouty arthritis, Lumbago, Sciatica, and Spondylitis.


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Hochberg MC, Altman RD, April KT, Benkhalti M, Guyatt G, McGowan J, et al. American college of rheumatology 2012 recommendations for the use of nonpharmacologic and pharmacologic therapies in osteoarthritis of the hand, hip, and knee. Arthritis Care Res 2012;64:465-74.

Radha MS, Gangadhar MR. Prevalence of knee osteoarthritis patients in Mysore city, Karnataka. Int J Rec Sci Res 2015;6:3316-20.

Mahajan A, Verma S, Tandon V. Osteoarthritis. JAPI 2005;53:634-41.

Tallheden T, Bengtsson C, Brantsing C, Carlsson L, Peterson L, Brittberg M, et al. Proliferation and differentiation potential of chondrocytes from osteoarthritic patients. Arthritis Res Ther 2005;7:560-8.

Scheinfeld N. A comprehensive review and evaluation of the side effects of the tumor necrosis factor alpha blockers etanercept, infliximab and adalimumab. J Dermatol Treat 2004;15:280-94.

Kongtharvonskul J, Anothaisintawee T, McEvoy M, Attia J, Woratanarat P, Thakkinstian A. Efficacy and safety of glucosamine, diacerein, and NSAIDs in osteoarthritis knee: A systematic review and network meta-analysis. Eur J Med Res 2015;20:24.

Tascioglu F, Armagan O, Tabak Y, Corapci I, Oner C. Low power laser treatment in patients with knee osteoarthritis. Swiss Med Wkly 2004;134:254-8.

Long L, Soeken K, Ernst E. Herbal medicines for the treatment of osteoarthritis: A systematic review. Rheumatology 2001;40:779-93.

Sawarkar G, Sawarkar P. Prevention and management of osteoarthritis. Int J Res Ayurveda Pharm 2013;4:454-8.

Akhtar B, Mahto RR, Dave AR, Shukla VD. Clinical study on Sandhigata Vata w.s.r. to osteoarthritis and its management by panchatikta ghrita guggulu. Ayu 2010;31:53-7.

Cao H, Yu R, Choi Y, Zhang H, Xiang W, Lee D, et al. Discovery of cyclooxygenase inhibitors from medicinal plants used to treat inflammation. Pharmacol Res 2010;61:519-24.

Alam M, Khan H, Samiullah L, Siddique KM. A review on phytochemical and pharmacological studies of Kundur (Boswellia serrata roxb ex colebr.)-a Unani drug. J Appl Pharm Sci 2012;2:148-56.

Shishodia S, Aggarwal B. Guggulsterone inhibits NF-B and I B kinase activation, suppresses expression of anti-apoptotic gene products, and enhances apoptosis. J Biol Chem 2004;279:47148-58.

Srivastava P. Chemical Standardization of Some Anti-inflammatory Rasayana Plants Used in Indian System of Medicine. Lucknow: Department of Chemistry University of Lucknow; 2013. p. 2-10.

Gacche R, Shaikh R, Pund M, Deshmukh R. Cyclooxygenase inhibitory, cytotoxicity and free radical scavenging activities of selected medicinal plants used in Indian traditional medicine. Pharmacogn J 2011;3:57-64.

Chattopadhyay P, Hazarika S, Dhiman S, Upadhyay A, Pandey A, Karmakar S, et al. Vitex Negundo inhibits cyclooxygenase-2 inflammatory cytokine-mediated inflammation on carrageenan-induced rat hind paw edema. Pharmacogn Res 2012;4:134-7.

Philip S, Tom G, Vasumathi AV. Evaluation of the anti-inflammatory activity of Tinospora cordifolia (Willd.) miers chloroform extract-a preclinical study. J Pharm Pharmacol 2018;70:1113-25.

Luseba D, Elgorashi EE, Ntloedibe DT, Staden JV. Antibacterial, anti-inflammatory and mutagenic effects of some medicinal plants used in South Africa for the treatment of wounds and retained placenta in livestock. South Afr J Bot 2007;73:378-83.

van Breemen RB, Tao Y, Li W. Cyclooxygenase-2 inhibitors in ginger (Zingiber officinale). Fitoterapia 2011;82:38-43.

Samson DJ, Grant MD, Ratko TA, Bonnell CJ, Ziegler KM, Aronson N. Treatment of primary and secondary osteoarthritis of the knee. Evid Rep Technol Assess 2007;157:1-157.

Dhingra M, Nain P, Nain J, Malik M. Hepatotoxicity v/s hepatoprotective agents-a pharmacological review. Int Res J Pharm 2011;2:31-7.

Bjorkman DJ. Current status of nonsteroidal anti-inflammatory drug (NSAID) use in the United States: Risk factors and frequency of complications. Am J Med 1999;107:3-8.

Sostress C, Gorgallo CJ, Lanas A. Nonsteroidal anti-infl ammatory drugs and upper and lower gastrointestinal mucosal damage. Arthritis Res Ther 2013;15:S3.

Aletaha D, Smolen JS. Laboratory testing in rheumatoid arthritis patients taking disease-modifying antirheumatic drugs: Clinical evaluation and cost analysis. Arthritis Rheum 2002;47:181-8.

Sharma SK, Goyal N. Biological studies of the plants from genus Pluchea. Ann Biol Res 2011;2:25-34.

Singh GB, Bani S, Singh S. Toxicity and safety evaluation of boswellic acids. Phytomedicine 1996;3:87-90.

Sharma JN, Sharma JN. Comparison of the anti-inflammatory activity of Commiphora mukul (an indigenous drug with those of phenylbutazone and ibuprofen in experimental arthritis induced by mycobacterial adjuvant. Arzneimittel Forschung 1977;27:1455-57.

Singh BB, Mishra LC, Vinjamury SP, Aquilina N. The effectiveness of Commiphora mukul for osteoarthritis of the knee: An outcomes study. Alternat Ther Health Med 2003;9:74-9.

Arya D, Patni V. Microprogagation of Pluchea lancelata (oliver and Hiren,)-a potent antiarthritic medicinal herb. Int J Med Arom Plants 2013;3:55-60.

Sumantran VN, Kulkarni A, Boddul S, Chinchwade T, Koppikar SJ, Harsulkar A, et al. Chondroprotective potential of root extracts of Withania somnifera in osteoarthritis. J Biosci 2007;32:299-307.

Rasool M, Varalakshmi P. Immunomodulatory role of Withania somnifera root powder on experimental induced inflammation: An in vivo and in vitro study. Vasc Pharmacol 2006;44:406-10.

Upadhyay AK, Kumar K, Kumar A, Mishra HS. Tinospora cordifolia (Willd.) Hook. F. and Thoms. (Guduchi)-validation of the ayurvedic pharmacological through experimental and clinical studies. Int J Ayurveda Res 2010;1:112-21.

Vishwanathan AS, Basavaraju R. A review on Vitex negundo L.-a medicinally important plant. EJBS 2010;3:30-42.

Dharmasiri MG, Jayakody A, Galhena G, Liyanage SS. Anti- inflammatory and analgesic activities of mature fresh leaves of Vitex negundo. J Ethnopharmacol 2003;87:199-206.

Gupta M, Mazumder UK, Bhawal SR. CNS activity of Vitex negundo Linn., in Mice. Indian J Exp Biol 1999;37:143-6.

Apaya KL, Chichioco-Hernandez CL. Xanthine oxidase inhibition of selected Philippine medicinal plants. J Med Plants Res 2011;5:289-92.

Devi PR, Kumari SK, Kokilavani C. Effect of Vitex negundo leaf extract on the free radicals scavengers in complete Freund’s adjuvant induced arthritic rats. Indian J Clin Biochem 2007;22:143-7.

Srivastava KC, Mustafa T. Ginger (Zingiber officinale) in rheumatism and musculoskeletal disorders. Med Hypotheses 1992;39:342-8.

Ojewole JA. Analgesic, antiinflammatory and hypoglycaemic effects of ethanol extract of Zingiber officinale (Roscoe) rhizomes (Zingiberaceae) in mice and rats. Phytother Res 2006;20:764-72.



How to Cite

NIPANIKAR, S., and C. SS. “EVALUATION OF ANALGESIC (IN VIVO) ACTIVITY OF ARIFLEX TABLET IN COMPARISON WITH DICLOFENAC AND ACECLOFENAC USING ACETIC ACID INDUCED WRITHING MODEL IN MICE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 14, no. 8, Aug. 2021, pp. 90-93, doi:10.22159/ajpcr.2021.v14i8.42188.



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