ISOLATION AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF MICROORGANISMS CAUSING INTRA-ABDOMINAL INFECTIONS AT TERTIARY CARE HOSPITAL OF NORTHERN INDIA

PARMINDER SINGH; RITU GARG; HARIT KUMAR; RINKU

doi:10.22159/ajpcr.2021.v14i10.42753

Authors

PARMINDER SINGH Department of Microbiology, Molecular Lab, RVRS Government Medical College, Bhilwara, Rajasthan, India.
RITU GARG Department of Microbiology, Maharishi Markandeshwar Institute of Medical Science and Research, Maharishi Markandeshwar University, Mullana, Haryana, India.
HARIT KUMAR Department of Microbiology, Maharishi Markandeshwar Institute of Medical Science and Research, Maharishi Markandeshwar University, Mullana, Haryana, India.
RINKU Department of Microbiology, Molecular Lab, RVRS Government Medical College, Bhilwara, Rajasthan, India.

DOI:

https://doi.org/10.22159/ajpcr.2021.v14i10.42753

Keywords:

Intra-abdominal infections, Methicillin-resistant Staphylococcus aureus, Empirical antimicrobials, Emerging resistance, Gram-negative organisms, Gram-positive organisms

Abstract

Objective: The objective of the study is to find out the resistance pattern of pathogenic organisms isolated from intra-abdominal infection (IAI).

Methods: A total of 500 samples were collected from suspected IAIs of patients reporting to the hospital and cultured. Identification of the isolates was done using standard identification protocol. Antimicrobial susceptibility was performed by Kirby-Bauer disc diffusion method and interpretation was done using Central Laboratory Standard Institute guidelines.

Results: Out of 500 samples, 170 were culture positive and 330 showed no growth. Gram-negative organisms (n=127) outnumbered the Gram-positive organisms (n=23). Among the Gram-negative organisms, Escherichia coli (n=67) was the most commonly isolated bacilli followed by Klebsiella sp. (n=32), Pseudomonas sp. (n=25), Acinetobacter baumannii (n=18), and Klebsiella oxytoca (n=05). Among Gram-positive organisms Staphylococcus aureus (n=17) and Enterococcus spp (n=06) isolates of were grown in culture. Among Gram-negative bacilli, Imipenem followed by Gentamicin was the most effective drug but in Acinetobacter spp. The second most effective drug was Tigecycline. Among Gram-positive isolates, Linezolid was the most effective drug.

Conclusion: Prompt starting of empirical antimicrobials based on the local susceptibility pattern, followed by modification of treatment in accordance with the antimicrobial susceptibility report can significantly reduce the morbidity and the mortality associated with IAIs.

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