TAXONOMIC CHARACTERIZATION, ANTIMICROBIAL ACTIVITY AND ANTIOXIDANT POTENTIAL OF RARE ACTINOBACTERIUM ISOLATED FROM MANGROVE ECOSYSTEM OF MACHILIPATNAM, ANDHRAPRADESH, INDIA

CHIRANJEEVI CH; VIJAYALAKSHMI M

doi:10.22159/ajpcr.2022.v15i3.43666

Authors

CHIRANJEEVI CH Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India. https://orcid.org/0000-0003-2482-965X
VIJAYALAKSHMI M Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2022.v15i3.43666

Keywords:

Mangrove actinomycete, Pseudonocardia parietis VLCH-6, Antimicrobial activity, Antioxidant potential, Bioactive compounds

Abstract

Objective: The objective of the present study is to isolate, characterize the potent actinomycete strain from mangrove habitats of South coast of Andhra Pradesh (Machilipatnam), India.

Methods: The samples collected from mangrove niches were pretreated with calcium carbonate, diluted, Plated on Starch casein agar, Humic acid vitamin agar and yeast extract malt extract dextrose (YMD) agar media to isolate actinomycetes. The three selective media were supplemented with clotrimazole (25 μg/mL) and streptomycin (25 μg/mL) to inhibit the growth of fungi and bacteria.

Results: A total of ten actinobacterial strains were isolated and designated as VLCH-1 to VLCH-10. All of them were screened and evaluated for their antimicrobial and antioxidant properties. Among them, one strain VLCH-6 was found to be potent as it exhibited high antimicrobial potential and it was identified as Pseudonocardia parietis VLCH-6 through polyphasic taxonomy (GenBank accession number: MZ951166). The growth profile of the strain was studied by culturing it in YMD broth. The culture broth extracted with ethyl acetate was tested for antimicrobial assay and found to be active against Escherichia coli, Xanthomonas campestris and Aspergillus niger. The antioxidant potential of the strain VLCH-6 was evaluated by 2,2- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. In the screening, the culture extract exhibited strong antioxidant activity against DPPH free radicals.

Conclusion: Over all, it was evident from the present study that mangrove habitats of Machilipatnam serve as a good source for potent actinomycetes possessing broad spectrum of antimicrobial and antioxidant metabolites.

Downloads

Download data is not yet available.

References

Palla MS, Guntuku GS, Muthyala MK, Pingali S, Sahu PK. Isolation and molecular characterization of antifungal metabolite producing actinomycete from mangrove soil. Beni Suef Univ J Basic Appl Sci 2018;7:250-6.

Lu QP, Ye JJ, Huang YM, Liu D, Liu LF, Dong K, et al. Exploitation of potentially new antibiotics from mangrove Actinobacteria in maowei sea by combination of multiple discovery strategies. Antibiotics (Basel) 2019;8:236.

Sharma M. Actinomycetes: Source, identification and their applications. Int J Curr App Sci 2014;3:801-32.

Mangamuri U, Muvva V, Poda S, Naragani K, Munaganti RK, Chitturi B, et al. Bioactive metabolites produced by Streptomyces cheonanensis VUK-A from Coringa mangrove sediments: Isolation, structure elucidation and bioactivity. 3 Biotech 2016;6:1-8.

Sengupta S, Pramanik A, Ghosh A, Bhattacharyya M. Antimicrobial activities of actinomycetes isolated from unexplored regions of Sundarbans mangrove ecosystem. BMC Microbiol 2015;15:170.

Indupalli MD, Muvva V, Munaganti RK, Mangamuri UK. Saccharomonospora oceani VJDS-3, A potent actinobacterial strain from mangrove ecosystem. Asian J Pharm Clin Res 2016;9:363-6.

Bhat SA, Nazir R, Malik TA, Shah FA. Secondary metabolites of actinomycetes as potential source of antibiotics. Stem Cells 2013;4:41-6.

Suresh RS, Younis EM, Fredimoses M. Isolation and molecular characterization of novel Streptomyces sp. ACT2 from marine mangrove sediments with antidermatophytic potentials. J King Saud Univ Sci 2020;32:1902-9.

Mangamuri UK, Muvva VV, Poda S, Kamma S. Isolation, identification and molecular characterization of rare actinomycetes from mangrove ecosystem of Nizampatnam. Malays J Microbiol 2012;8:83-91.

Tanvir R, Sajid I, Hasnain S, Kulik A, Grond S. Rare actinomycetes Nocardia caishijiensis and Pseudonocardia carboxydivorans as endophytes, their bioactivity and metabolites evaluation. Microbiol Res 2016;185:22-35.

Ying H, Wang L, Zhitang L, Hong L, Liu Z, Geok Y, et al. Proposal to combine the genera Actinobispora and Pseudonocardia in an emended genus Pseudonocardia, and description of Pseudonocardia zijingensis sp. nov. Int J Syst Evol Microbiol 2002;52:977-82.

Kavitha A, Vijayalakshmi M, Sudhakar P, Narasimha G. Screening of actinomycete strains for the production of antifungal metabolites. Afr J Microbiol Res 2011;4:27-32.

Muvva V, Munaganti RK, Naragani K, Konda S, Dorigondla KR. Production of antimicrobial metabolites by Streptomyces lavendulocolor VHB-9 isolated from granite mines. BSSN HB. Braz Arch Biol Technol 2017;60:V60.

Mogili MS, Muvva V. Selective isolation and characterization of Nocardiopsis flavescens vjms-18 from coastal regions of Andhra Pradesh, India. Int J Pharmacol Res 2019;10:1000-8.

Janaki T. Antimicrobial activity of mangrove actinomycetes from soil sample of Rhizophora apiculata. J Biotech Res 2016;1:44-52.

Gebreyohannes G, Moges F, Sahile S, Raja N. Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethipia. Asian Pac J Trop Biomed 2013;3:426-35.

Lakshman M. Application of conventional electron microscopy in aquatic animal disease diagnosis. A review Mekala Lakshman. J Entomol Zool Stud 2019;7:470-5.

Mantada PK, Sankar GC, Prabhakar GT. Isolation and characterization of potent antibiotic producing marine actinomycetes from tiruchendur and kulasekarapattinam, Tamil Nadu. Glob J Sci Front Res 2013;13:1-5.

Yasmeen S, Vijayalakshmi M, Kumar MR. Taxonomic characterization of rare Actinobacteria isolated from mangrove ecosystem. Int J Curr Res 2016;8:31060-6.

Reddy NG, Ramakrishna DP, Rajagopal SV. A morphological physiological and biochemical studies of Streptomyces rochei MTCC 10109 showing antagonistic activity against selective human pathogenic microorganisms. Asian J Biol Sci 2011;4:1-14.

Ragalatha R, Vijayalakshmi M, Mallikarjuna K, Kishore IV. Bioactivity and taxonomic studies of Nocardiopsis dassonvillei and Nocardiopsis species from Marine sediments of Bay of Bengal, India. Int J Pharm Sci Res 2020;1:1790-801.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-29.

Jafari N, Behroozi R, Farajzadeh D, Farsi M, Akbari-Noghabi K. Antibacterial activity of Pseudonocardia sp. JB05, A rare salty soil actinomycete against Staphylococcus aureus. BioMed Res Int 2014;2014:182945.

Kapur MK, Solanki R, Das P, Kumar M, Kumar P. Antimicrobial activity analysis of bioactive compounds from soil actinomycetes. J Pharm Chem BiolSci 2018;6:178-87.

Tan LT, Chan KG, Pusparajah P, Yin WF, Khan TM, Lee LH, et al. Mangrove derived Streptomyces sp. MUM265 as a potential source of antioxidant and anticolon-cancer agents. BMC Microbiol 2019;19:38.

Ryandini D, Radjasa OK, Oedjijono O. Isolate actinomycetes SA32 origin of Segara Anakan mangrove rhizosphere and its capability in inhibiting multi-drugs resistant bacteria growth. J Microb Biochem Technol 2018;10:1-7.

Govindarajan G, Santhi VS, Jebakumar SR. Antimicrobial potential of phylogenetically unique actinomycete, Streptomyces sp. JRG-04 from marine origin. Biologicals 2014;42:305-11.