PREVENTION OF DNA SUGAR, HUMAN PERIPHERAL LYMPHOCYTES AND ERYTHROCYTES DAMAGES FROM FREE RADICAL INDUCED OXIDATION BY NATURAL ANTIOXIDANTS

OMKAR NG; PREM KUMAR G; BHAGYALAKSHMI V; DINESHA RAMADAS

doi:10.22159/ajpcr.2022.v15i3.43726

Authors

OMKAR NG Department of Physiology, Shridevi Institute of Medical Sciences and Research Hospital, Tumkur, Karnataka, India.
PREM KUMAR G Department of Biochemistry, Andra Medical College, Visakhapatnam, Andhra Pradesh, India.
BHAGYALAKSHMI V Department of Biochemistry, Rangaraya Medical College, Kakinada, Andhra Pradesh, India.
DINESHA RAMADAS Department of Biochemistry, Adichunchanagiri Institute for Molecular Medicine, Central Research Laboratory-Adichunchanagiri Institute of Medical Sciences, Adichunchanagiri University, Mandya, Karnataka, India.

DOI:

https://doi.org/10.22159/ajpcr.2022.v15i3.43726

Keywords:

DNA, Lymphocytes, Fenton, Erythrocytes, Agathi, Antioxidants

Abstract

Objective: The present study focused on the identification of particular extract which shows signification protection of DNA sugar against excessive oxidation.

Methods: The different extracts (water, alcohol, alcohol: water, and hexane) of Agathi seeds (Sesbania grandiflora Linn) were evaluated using various antioxidant and other relevant assays like DNA sugar protection and antioxidant activities.

Results: The alcohol: water (1:1) extract of S. grandiflora Linn seeds showed the highest antioxidant and free radical scavenging activity. It inhibited membrane lipid peroxidation by 55% at 50 μg/ml, scavenged approximately 69% of hydroxyl and 1,1-diphenyl-2-picrylhydrazayl radicals at 2–3 fold lower concentrations compared to the other extracts. In addition, the alcohol: water extract inhibited ferrous sulfate: ascorbate-induced sugar oxidation of DNA and also showed non-toxic nature against lymphocytes.

Conclusion: These results establish the antioxidant potential of the extract, which could be used as natural antioxidant source.

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