A STUDY ON BACTERIOLOGICAL PROFILE AND ANTIBIOTIC SUSCEPTIBILITY PATTERN OF SURGICAL SITE INFECTIONS IN A TERTIARY CARE HOSPITAL

PURIMITLA USHARANI

doi:10.22159/ajpcr.2022.v15i6.44485

Authors

PURIMITLA USHARANI Department of Microbiology, Dr. Patnam Mahender Reddy Institute of Medical Sciences, Rangareddy, Telangana, India.

DOI:

https://doi.org/10.22159/ajpcr.2022.v15i6.44485

Keywords:

Surgical site infections, Demographic variables, Culture, Monomicrobial infection, Polymicrobial infection, Antibiotic susceptibility

Abstract

Objective: The present study was aimed to evaluate the prevalence, microbiological profile, and antibiograms of pathogenic microorganisms causing surgical site infections.

Methods: The present study was conducted in the Department of Microbiology, Dr. Patnam Mahender Reddy Institute of Medical Sciences, Telangana. The study was conducted from the period of April 2020 to April 2021. Three hundred pus samples were analyzed for culture isolation and identification using standard protocols. Antibiotic susceptibility pattern of each isolate was performed by standard disc diffusion method by following The Clinical and Laboratory Standard Institute guidelines.

Results: A total of monomicrobial isolates 165 (55%) and polymicrobial isolates 13 (4.4%) were obtained from 300 pus samples and remaining 122 (40.6%) pus samples were sterile. The predominant bacteria were Staphylococcus aureus 50 (30.3%) followed by Pseudomonas aeruginosa 39 (23.6%) in monomicrobial infection and Klebsiella oxytoca + P. aeruginosa 3 (23%) in polymicrobial infection. The results of antibiogram of S. aureus which showed the highest sensitivity to antibiotics like linezolid 47 (94%) and doxycycline 42 (80%) compared to other antibiotics used for antibiotic susceptibility testing. Among the Gram-negative bacteria, the prevalent organism was Klebsiella species which showed the highest sensitivity to antibiotic meropenem 32 (86.5%).

Conclusion: The predominant bacterium isolated was S. aureus followed by P. aeruginosa in monomicrobial infection and K. oxytoca + P. aeruginosa in polymicrobial infection. Controlling the morbidity of surgical site infections is aided by meticulous surgical procedures, careful sterilization, judicious use of antibiotics, improved operating theater and ward conditions, control of malnutrition and obesity, management of infective foci, and diseases such as diabetes.

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References

Török ME, Conlon CP. Skin and soft tissue infections. Medicine 2013;41:709-15. doi: 10.1016/j.mpmed.2013.10.001

Sujata S, Mayuri K, Rakeshkumar P, Swati B, Sahare KN. Antibacterial activities against pyogenic pathogens. Int J Pharm Sci Res 2013;4:2974-9.

Trojan R, Razdan L, Nasib S. Antibiotic susceptibility patterns of bacterial isolates from pus samples in a tertiary care hospital of Punjab, India. Int J Microbiol 2016 9302692:1-4.

Rawat S, Saxena N, Jain P. Aerobic bacterial profile and antimicrobial susceptibility pattern of pus isolates in a tertiary care hospital in Hadoti region. Int J Curr Microbiol Appl Sci 2017;6:2866-73. doi: 10.20546/ ijcmas.2017.605.326

Becker K, Heilmann C, Peters G. Coagulase-negative staphylococci. Clin Microbiol Rev 2014;27:870-926. doi: 10.1128/CMR.00109-13, PMID 25278577

Dryden MS. Complicated skin and soft tissue infection. J Antimicrob Chemother 2010;65:iii35-44. doi: 10.1093/jac/dkq302, PMID 20876627

Krishna Sarin MS, Vadivelan M, Chanaveerappa B. Antimicrobial therapy in the intensive care unit. Ind J Clin Prac 2013;23:601-9.

Collee JG, Fraser AG, Marmion BP, Simmons A. Mackie and McCartney Practical Medical Microbiology. 14th ed. United Kingdom: Elsevier, Church Hill Livingstone; 2006.

Khatoon R, Khan SA, Jahan N. Antibiotic resistance pattern among aerobic bacterial isolates from osteomyelitis cases attending a tertiary care hospital of North India with special reference to ESBL, AmpC, MBL and MRSA production. Int J Res Med Sci 2017;5:482-90. doi: 10.18203/2320-6012.ijrms20170137

Zhang S, Ren L, Li Y, Wang J, Yu W, Li N, et al. Bacteriology and drug susceptibility analysis of pus from patients with severe intra-abdominal infection induced by abdominal trauma. Exp Ther Med 2014;7:1427- 31. doi: 10.3892/etm.2014.1609, PMID 24940451

Naz R, Hussain SM, Ain QU. Bacteriological profile of surgical site infections and their antibiotic susceptibility pattern. SSR inst. Int J Life Sci 2019;5:2224-9.

Fatema K, Mondol K, Akter T, Datta S. Detection of pus sample of various pyogenic infections from diabetic and non diabetic patient and compare their socioeconomic status in Bangladesh. Am Sci Res J Eng Technol Sci 2016;26:159-65.

Ahmed M, Alam SN, Khan O, Manzar S. Post-operative wound infection: A surgeon’s dilemma. Pak J Surg 2007;23:41-7.

Mulu W, Kibru G, Beyene G, Damtie M. Post-operative nosocomial infections and antimicrobial resistance pattern of bacteria isolates among patients admitted at Felege Hiwot referral hospital, Bahirdar, Ethiopia. Ethopian J Health Sci 2012;22:7-18.

Santosh S, Gupta N, Aparna, Lokveer, Griwan MS. Surgical infections: A microbiological study. Braz J Infect Dis 2004;8:118-25.

Poonam V. A study on isolation of different type of bacteria from pus. Int J Pharm Life Sci 2012;3:2107-10.

Nwachukwu NC. Antibiotic susceptibility patterns of bacterial isolates from surgical wounds in Abia State University teaching hospital (ABSUTH), Aba Nigeria. Res J Med Med Sci 2009;4:575-9.

Dinda V, Gunturu R, Kariuki S. Pattern of pathogens and their sensitivity isolated from surgical site infections at the Agha khan university hospital, Nairobi, Kenya. Ethiop J Health Sci 2013;23:141-9.

Anguzu JR, Olila D. Drug sensitivity patterns of bacterial isolates from septic post-operative wounds in a regional referral hospital in Uganda. Afr Health Sci 2007;7:148-54. doi: 10.5555/afhs.2007.7.3.148, PMID 18052868

Mahmood A. Bacteriology of surgical site infections and antibiotic susceptibility pattern of the isolates at a tertiary care hospital in Karachi. J Pak Med Assoc 2000;50:256-9. PMID 10992708

Ahmed J, Amjad A, Hussain M. Antimicrobial susceptibility pattern of bacteria isolated from burn wounds in a tertiary care hospital in Pakistan. Afr J Microbiol Res 2013;7:3627-31.

Mehta VJ, Kikani KM, Mehta SJ. Microbiological profile of diabetic foot ulcers and its antibiotic susceptibility pattern in a teaching hospital, Gujarat. Int J Basic Clin Pharmacol 2014;3:92-5. doi: 10.5455/2319- 2003.ijbcp20140209

Abdelraouf AE. Antimicrobial resistance of Acinetobacter spp. Isolated from pus specimens from Al-Shifa hospital, Gaza, Palestine. J Al-Aqsa Univ 2006;10:59-68.

Adegoke AA, Tom M, Okoh AI, Jacob S. Studies on multiple antibiotic resistant bacterial isolated from surgical site infection. Sci Res Essays 2010;5:3876-81.

Ahmed S, Aslam N, Mehdi N. Bacterial profile of surgical site infections and their antibiograms in patients undergoing coronary artery bypass graft surgery. Int J Contemp Med Res 2020;7:1-4.