HLA-DRB1 ALLELE DISTRIBUTION IN HEALTHY INDIVIDUALS CLASSIFIED BASED ON MIZAJ (TEMPERAMENT) OF UNANI SYSTEM OF MEDICINE

GHAZALA MULLA; TEJAS SHAH; KALPANA JOSHI; JALIS AHMED; FARHAN QURESHI

doi:10.22159/ajpcr.2022.v15i8.44832

Authors

GHAZALA MULLA Department of Physiology, Z V M Unani Medical College and Hospital, Pune, Maharashtra, India. https://orcid.org/0000-0002-5396-6357
TEJAS SHAH Department of Biotechnology, Sinhgad College of Engineering, Affiliated to Savitribai Phule Pune University, Vadgaon Budruk, Pune, Maharashtra, India.
KALPANA JOSHI Department of Biotechnology, Sinhgad College of Engineering, Affiliated to Savitribai Phule Pune University, Vadgaon Budruk, Pune, Maharashtra, India.
JALIS AHMED Department of Biotechnology, Sinhgad College of Engineering, Affiliated to Savitribai Phule Pune University, Vadgaon Budruk, Pune, Maharashtra, India.
FARHAN QURESHI Department of Physiology, Z V M Unani Medical College and Hospital, Pune, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2022.v15i8.44832

Keywords:

Mizaj, Temperament, Sheldon’s somatoplot, Polymerase chain reaction-sequence-specific typing, Human leukocyte antigen-DRB1, Disease management

Abstract

Objective: Assessment of Mizaj of 500 healthy individuals between the age group of 25and 40 years of the age group of either gender was carried out by validated Unani questionnaire and Sheldon’s somatotype. The present study was designed to investigate if the frequency of HLA-DRB1 alleles in the healthy adult population has any association with four Mizaj.

Methods: Mizaj of the healthy individuals (n=500) was assessed by a validated Unani questionnaire based on 10 parameters related to phenotypic and psychological entities known as Ajnas-e-Ashra. Mizaj of the same subjects was further assessed by Sheldon’s somatoplot. Polymerase chain reaction-sequence-specific typing method was used for human leukocyte antigen (HLA) genotyping of 180 healthy volunteers.

Conclusion: An expected correlation between HLA-DRB1 alleles and Mizaj types is observed. Five single-nucleotide polymorphisms (p<0.05) were significantly associated with different Mizaj. The study suggests that the Mizaj classification of the Unani system of medicine has a genetic basis. This study would be one step toward a personalized approach as per the Mizaj of an individual to disease proneness, lifestyle modification, and medicine.

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