HEPATOPROTECTIVE ACTIVITY OF Trichosanthes cucurmena L.
Abstract
Objective: This study investigated the hepatoprotective activity of ethanolic extract of Trichosanthes cucumerina L. (EETC), in paracetamol induced
hepatotoxic studies carried out in male albino rats and included to produce toxicity of the formulation.
Methods: Albino rats (175 g) were taken and divided into four groups liver necrosis was induced by intraperitoneal injection of paracetamol (3 mg/
kg b.w, p.o). The hepatoprotective activity of EETC was evaluated by measuring levels of serum marker enzymes like serum glutamate oxaloacetate
transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and alkaline phosphatase (ALP). The serum levels of total proteins and
bilirubin were also estimated. The histological studies were carried out to support the above parameters. Here Silymarin was used as standard drug.
Result: Administration of EETC (150 mg/kg, p.o.) markedly prevented paracetamol-induced elevation of levels of SGPT, SGOT, ALP, and bilirubin. The
decreased level of total proteins due to hepatic damage induced by paracetamol was found to be increased in EETC treated group.
Conclusion: The results are comparable to the of silymarin. A histopathological study of liver exhibited almost normal architecture when compared
with paracetamol treated group. Hence the hepatoprotective activity of EECT was proved.
Keywords: Trichosanthes cucumerina L., Serum glutamate oxaloacetate transaminase, Serum glutamate pyruvate transaminase, Sylimarin and
Paracetamol.
Downloads
References
Padh H, Patel B. Herbal drugs. Curr Sci 2001;81:15.
Thaibinh T. Herbal medicine. Indian J Pharm Educ 1998;32:104-6.
Chandira M, Jayakar B. Formulation and evaluation of herbal tablets containing Ipomoea digitata Linn. Extract. Int J Pharm Sci Rev Res 2010;3:101-10.
Nadkarni KM. Indian Materia Medica. Mumbai: Popular Prakashan Pvt. Ltd.; Vol. I . 2002. p. 223-5.
Vishnu SN, Gaikwadkuldeep G, Deepak BG. Hepatoprotective activity of Cassia alata Linn. in rifampicin indued liver injury in rats. Adv Pharm Toxicol 2010;11:27-36.
Ahamed SM, Jayaveera KN. Rao V, Tukura N. Hepatoprotective activity and antioxidant activity of ethanolic extract of Feronialimonia leaves on paracetamol induced hepatic injury in rats. Adv Pharm Toxicol 2010;11:47.
Gujrati V, Patel N, Rao VN, Nandakumar K, Gouda TS, Shalam MD, Shantakumar SM. Hepato protective activity of alcoholic and aqueous extract of leaves of TylophoraIndica Linn in rats. Adv Pharm Toxicol 2009;10(1):136-42.
Praveen TK, Dharmaraj S, Bajaj J, Dhanabal SP, Manimaran S, Nanjan MJ, et al. Hepatoprotective activity of petroleum ether, diethyl ether, and methanol extract of Scoparia dulcis L. against CCl4-induced acute liver injury in mice. Indian J Pharmacol 2009;41(3):110‑4.
Slater TF. Biochemical Mechanism of Liver Injury. London: Academic Press; 1965. p. 1-54.10. Sallie R, Tredger JM, Williams R. Drugs and the liver. Part 1: Testing liver function. Biopharm Drug Dispos 1991;12(4):251-9.
Ashok SK, Somayaji SN, Bairy KL. Hepatoprotective effects of Ginkgo biloba against carbon tetrachloride induced hepatic injury in rats. Indian J Pharmacol 2001;33(2):260-6.
Galighor AE, Kozloff EN. Biochemistry and molecular biology. In: Essentials of Practical Micro Technique. 2nd ed. New York: Lea and Febiger; 1976.
Galighor AE, Kozloff EN. In: Essentials of Practical Micro Technique. 2nd ed. New York: Lea and Febiger; 1976.
Das S, Roy P, Auddy RG, Mukherjee A. Silymarin nanoparticle prevents paracetamol-induced hepatotoxicity. Int J Nanomedicine 2011;6:1291‑301.
Koch RR, Glende EA Jr, Recknagel RO. Hepatotoxicity of bromotrichloromethane – bond dissociation energy and lipoperoxidation. Biochem Pharmacol 1974;23(20):2907-15.
Carvalho M, Carvalho F, Bastos ML. Is hyperthermia the triggering factor for hepatotoxicity induced by 3,4-methylenedioxymethamphetamine (ecstasy)? An in vitro study using freshly isolated mouse hepatocytes. Arch Toxicol 2001;74:789-93.
Shibayama Y. Role of lipid peroxidation in enhancement of endotoxin hepatotoxicity. Exp Toxicol Pathol 1992;44(4):205-8.
Shtukmaster S, Ljubuncic P, Bomzon A. The effect of an aqueous extract of Teucrium polium on glutathione homeostasis in vitro: A possible mechanism of its hepatoprotectant action. Adv Pharmacol Sci 2010;2010:938324.
Iriadam M, Musa D, Gumushan H, Baba F. Effects of two Turkish medicinal plants Artemisia herba-alba and Teucrium polium on blood glucose levels and other biochemical parameters in rabbits. J Cell Mol Biol 2006;5:19-24.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.