ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF CRUCIFEROUS VEGETABLES - CAULIFLOWER, BROCCOLI

SUNDAR S; JAYARAMI REDDY A

doi:10.22159/ajpcr.2023.v16i2.46346

Authors

SUNDAR S Department of Pharmacology, Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, Vijayawada, Krishna, Andhra Pradesh, India.
JAYARAMI REDDY A Department of Pharmacology, Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, Vijayawada, Krishna, Andhra Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2023.v16i2.46346

Keywords:

Cruciferous vegetables, Antibacterial, Antifungal, Agar disk diffusion

Abstract

Objectives: This work aims to analyze the antibacterial and antifungal activities of cruciferous vegetables such as cauliflower and broccoli.

Methods: Cruciferous vegetables act as a good source of natural antioxidants due to their high levels of carotenoids, tocopherols, and ascorbic acid. In this study, two cruciferous vegetables, such as cauliflower and broccoli, were selected for antibacterial and anti-fungal studies. The stems, flowers of cauliflower, and broccoli were extracted with 125 mL of ethanol and water by Soxhlet’s apparatus for 6 h. Mueller Hinton agar and Sabouraud’s dextrose agar medium were used for antibacterial and antifungal activity, respectively. The antibacterial and antifungal activities of each cauliflower, broccoli stem, and flower extract were determined using a modified Kirby–Bauer disk diffusion method. Standard antibiotics, gentamicin (25 μg/mL), and fluconazole (25 μg/mL) served as positive controls for antibacterial and antifungal activity, respectively.

Results: Broccoli stem (100 μg/mL) ethanol extract produced higher antibacterial activity (13 mm) against Escherichia coli. Cauliflower, flower (100 μg/mL) ethanol extract produced higher antibacterial activity (13 mm) against Staphylococcus aureus. Broccoli flower (100 μg/mL) ethanol extract produced higher antifungal activity (14 mm) against Candida albicans. According to the results obtained from this project, broccoli stems and flower ethanol extracts show very good antibacterial activity against Gram-negative microorganisms such as E. coli and Pseudomonas aeruginosa. Similarly, cauliflower, flower ethanol extract shows excellent antibacterial activity against Gram-positive microorganisms such as Bacillus subtilis and S. aureus.

Conclusion: Further analysis is recommended for the identification of active constituents responsible for these activities.

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References

Sofowora A. Medicinal Plants and Traditional in Africa. New York: John Willy; 1982. p. 289-90.

Westh H, Zinn CS, Rosdahl VT. An International multicenter study of Antimicrobial consumption and resistance in Staphylococcus aureus isolated from 15 hospitals in 14 countries. Microbe Drug Resist 2004;10:169-76. doi: org/10.1089/1076629041310019

Hill AF. Economic Botany. A Textbook of Useful Plants and Plant Products 2nd ed. New York: Mcgraw-Hill Book Company Inc.; 1952.

Mithen R. Glucosinolates-biochemistry, genetics, and biological activity. Plant Growth Regul 2001;34:91-103. doi: org/10.1023/A:1013330819778

Jahangir M, Kim HK, Choi YH, Verpoorte R. Health affecting compounds in Brassicaceae. Compr Rev Food Sci Food Saf 2009;8:31- 43. doi: org/10.1111/j.1467-789X.2010.00790.x

Vallejo F, Tomas-Barberan FA, Garcıa-Viguera C. Phenolic compound contents in edible parts of broccoli inflorescences after domestic cooking. J Sci Food Agric 2003;83:1511-6. doi: org/10.1002/jsfa.1183

Heimler D, Vignolini P, Dini MG, Vincieri FF, Romani A. Antiradical activity and polyphenol composition of local Brassicaceae edible varieties. Food Chem 2006;99:464-9. doi: org/10.1016/j.foodchem.2005.07.057

Goel U, Kawatra BL, Bajaj S. Nutrition evaluation of a cauliflower leaf protein. J Sci Food Agric 1997;28:786-90. doi: org/10.1002/ jsfa.2740280903

Chang CT, Hseueh YL, Shung HY. Purification and properties of Chitinase from cabbage stems with roots. Biochem Mol Biol Int 1996;40:417-25. doi: org/10.1080/15216549600201922

Fahey JW, Zhang Y, Talalay P. Broccoli sprouts: An exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proc Natl Acad Sci U S A 1997;94:10367-72. doi: org/10.1073/ pnas.94.19.10367

Nestle M. Broccoli sprouts in cancer prevention. Nutr Rev 1998;56:127- 30. doi: org/10.1111/j.1753-4887.1998.tb01725.x

Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized disk method. Am J Clin Pathol 1966;45:493-6.