POSSIBLE INVOLVEMENT OF PEROXIDATIVE STRESS AND ANTIOXIDANT LEVELS IN ALCOHOLIC AND NON-ALCOHOLIC HEALTHY SUBJECTS
DOI:
https://doi.org/10.22159/ajpcr.2023.v16i10.47874Keywords:
Alcoholics, Peroxidative stress, Malondialdehyde, Superoxide dismutase, Catalase, Glutathione, Glutathione peroxidaseAbstract
Objectives: Antioxidant levels vary from person-to-person depending on their degree and type of activity, food, exposure to psychological stress, and contaminated environment. In addition, those who are older, have specific medical conditions, take drugs, smoke, and consume alcohol, and are exposed to solar radiation may require more carotenoids and other antioxidants. This led us to compare the antioxidant levels within alcoholics and non-alcoholic healthy subjects.
Methods: Malondialdehyde (MDA) and level of some antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione (GSH), antioxidant Vitamin A, Vitamin C, and Vitamin E were estimated in the circulations of alcoholics and non-alcoholic healthy subjects.
Results: Significantly increased concentrations of plasma thiobarbituric acid reactive substances, and significantly lowered levels of SOD, CAT, GSH, and GSH-Px were observed in alcoholics and may be due to their increased utilization to scavenge lipids peroxides. Increased levels of lipid peroxidation may be due to excessive oxidative stress. The comparison between alcoholics and non-alcoholics revealed 24% increased MDA in alcoholic subjects. Enzymatic (SOD, CAT, and GSH-Px), metabolic (GSH), and nutrient antioxidants (Vitamin A, E, C, and β-carotene) were lower in alcoholic as compared to non-alcoholic subjects.
Conclusion: Regardless of commercial brand, alcohol raises oxidative stress. When drinking alcohol and smoking or environmental contamination are coupled, this stress is significantly greater.
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References
Lieber CS. Alcoholic liver disease: New insights in pathogenesis lead to new treatments. J Hepatol 2000;32 Suppl:113-28. doi: 10.1016/s0168- 8278(00)80420-1, PMID 10728799
Osna NA, Donohue TM Jr., Kharbanda KK. Alcoholic liver disease: Pathogenesis and current management. Alcohol Res 2017;38:147-61. PMID 28988570
Singh RB, Ghosh S, Niaz MA, Rastogi V and Wander GS. Validation of tobacco and alcohol intake questionnaire in relation to food intakes for the Five City Study and a proposed classification for Indians. J Assoc Physicians India 1998;46:587-91. PMID 12152836
Patil AM, Mohammed AK, Saeed M and Sajanar BB. Study of alcoholic liver cirrhosis in hospital based patients, Bijapur, Northern Karnataka, India. Int J Curr Med Appl Sci 2015;7:16-20.
Singh M, Gupta S, Singhal U, Pandey R, Aggarwal SK. Evaluation of the oxidative stress in chronic alcoholics. J Clin Diagn Res 2013;7:1568-71. doi: 10.7860/JCDR/2013/5596.3210, PMID 24086841
Crabb DW. Pathogenesis of alcoholic liver disease: Newer mechanisms of injury. Keio J Med 1999;48:184-88. doi: 10.2302/ kjm.48.184, PMID 10638142
Crawford JM. Histologic findings in alcoholic liver disease. Clin Liver Dis 2012;16:699-716. doi: 10.1016/j.cld.2012.08.004, PMID 23101978
Theise ND. Histopathology of alcoholic liver disease. Clin Liver Dis (Hoboken) 2013;2:64-7. doi: 10.1002/cld.172, PMID 30992826
Gollnick HP, Hopfenmuller W, Hemmes C, Chun SC, Schmid C, Sundermeir K, Biesalski HK. Systemic beta carotene plus topical UV-sunscreen are an optimal protection against harmful effects of natural UV-sunlight: Results of the Berlin-Eilath study. Eur J Dermatol 1996;6:200-5.
Buege JA, Aust SD. Microsomal lipid peroxidation: The thiobarbituric acid assay. Methods Enzymol 1978;52:306.
Mishra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Biol Chem 1972;3170:27.
Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389-94. doi: 10.1016/0003-2697(72)90132-7, PMID 4556490
Pirie A. Glutathione peroxidase in lens and a source of hydrogen peroxide in aqueous humour. Biochem J 1965;96:244-53. doi: 10.1042/ bj0960244, PMID 14343138
Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61:882-8. PMID 13967893
Natelson S. Techniques of Clinical Chemistry. 3rd ed. Vol. P162. United States: Charles C. Thomas; 1971. p. 258, 751.
Roe JH, Kuether CA. A color reaction for dehydroascorbic acid useful in the determination of vitamin C. Science 1942;95:77. doi: 10.1126/ science.95.2455.77, PMID 17791074
Baker H, Frank O. Cinical Vitaminology: Methods and Interpretation. New York: Interscience Publishers, John Wiley and Sons Inc; 1968. p. 172.
Feher J, Csomos G, Vereckei A. Role of free radical reactions in liver diseases: In: Csomos G, Feher J. Free Radicals and the Liver. Germany: Springer; 1992. p. 1-17.
Seis H, Norman IK. The present status of antioxidant vitamin and beta-carotene. Am J Clin Nutr 1995;62:1299-300.
Lecomte E, Herbeth B, Pirollet P, Chancerelle Y, Arnaud J, Musse N, et al. Effect of alcohol consumption on blood antioxidant nutrients and oxidative stress indicators. Am J Clin Nutr 1994;60:255-61. doi: 10.1093/ajcn/60.2.255, PMID 8030604
Bjørneboe GE, Johnsen J, Bjørneboe A, Marklund SL, Skylv N, Høiseth A, et al. Some aspects of antioxidant status in blood from alcoholics. Alcohol Clin Exp Res 1988;12:806-10. doi: 10.1111/j.1530- 0277.1988.tb01350.x, PMID 3064642
Jeffrey AN, Ronald QT. Hepatic ethanol metabolism is mediated predominantly by catalase-H2O2, in the tasted state. FEBS Lett 1988;238:139-41.
Leevy CM, Thompson A, Baker H. Vitamins and liver injury. Am J Clin Nutr 1970;23:493-9.
Hagen BF, Bjørneboe A, Bjørneboe GE, Drevon CA. Effect of chronic ethanol consumption on the content of alpha-tocopherol in subcellular fractions of rat liver. Alcohol Clin Exp Res 1989;13:246-51. doi: 10.1111/j.1530-0277.1989.tb00321.x, PMID 2658665
Ingold KU, Webb AC, Witter D, Burton GW, Metcalfe TA, Muller DP. Vitamin E remains the major lipid-soluble, chain-breaking antioxidant in human plasma even in individuals suffering severe vitamin E deficiency. Arch Biochem Biophys 1987;250:224-6.
Beyer RE. The role of ascorbate in antioxidant protection of biomembrane: Interaction with vitamin E and coenzyme Q. J Bioenerg Biomembr 1994;26:349-58. doi: 10.1007/BF00762775, PMID 7844109
Retsky KL, Freeman MW, Frei B. Ascorbic acid oxidation products protects human low density lipoprotein against atterogenic modification. J Biol Chem 1993;268:1304-9. doi: 10.1016/S0021-9258(18)54075-8, PMID 8419332
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