EVALUATION OF ANTI-ANXIETY ACTIVITY OF SILICA GEL ENTRAPPED TRADITIONAL MEDICINE
Abstract
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Objectives: The present study has been designed to compare the anxiolytic property of Makaradhwaja (a well-known traditional Ayurvedic medicine)
and gel encapsulated Makaradhwaja in laboratory animals.
Methods: Makaradhwaja has been encapsulated into silica gel matrix to enhance the bioavailability and potency. The proper formation of the gel has
been evidenced by the transmission electron microscopy micrograph and Fourier transform infrared (FTIR) spectrometry. Due to the presence of
Makaradhwaja into silica gel matrix broad peaks at 3474 cm–1 and 1637 cm–1 were detected in FTIR spectrum. The UV-visible spectroscopic analysis
reveals sustainable and controlled release (up to 50%) of the drug for more than 240 hrs. The anti-anxiety activity was studied by exposing the mice
into two different anti-anxiety models like elevated plus maze (EPM) and actophotometer.
Results: The gel encapsulated Makaradhwaja has shown more potent anxiolytic effect than the crude Makaradhwaja in the EPM (significant increase
in the number of entries and time spent in the open arm). The number of locomotor score in actophotometer has also been significantly increased by
the gel encapsulated Makaradhwaja.
Conclusion: Present study confirms that the gel encapsulated Makaradhwaja has significant anxiolytic activity which is comparable with standard
anxiolytic diazepam.
Keywords: Actophotometer, Makaradhwaja, Elevated plus maze.
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