DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROSCOPIC METHOD FOR ESTIMATION OF RIZATRIPTAN BENZOATE IN BULK AND TABLET DOSAGE FORM

Abstract

 Objective: Development of a simple, precise, accurate, specific, and robust UV-visible spectrophotometric method for the quantitative determination of rizatriptan benzoate in tablets and bulk form.

Method: The method is developed using phosphate buffer pH 6.8 as a solvent. The stock solution of rizatriptan benzoate was prepared in phosphate buffer pH 6.8 and subsequent dilution was done in phosphate buffer pH 6.8. The standard solution of rizatriptan benzoate showed absorption maxima at 226 nm. The sample was prepared by simple extraction method.

Results: The drug obeyed Beer–Lambert's law and linearity was studied in the concentration range of 1–8 μg/ml with correlation coefficient 0.9956 at 226 nm. The limit of detection and limit of quantification were found to be 0.00368μg/ml and 0.01126μg/ml, respectively. The accuracy of the method was checked by recovery experiment performed at three different levels i.e., 80percente, 100percente and 120 percente. The percente recovery was found to be in the range 98.75-100.52percente. The low values of percente relative standard deviation (RSD) are indicative of the accuracy and reproducibility of the method. The precision of the method was studied as an intra-day, inter-day variations and repeatability. The percente RSD value less than 2 indicate that the method is precise.

Conclusion: The above method was a cost-effective quality-control tool for routine analysis of rizatriptan benoate in bulk and in pharmaceutical dosage form.

 

Keywords: Absorption, Beer–Lambert's law, Percent RSD, Rizatriptan benzoate, UV visible spectrophotometric.