ANTIOXIDANT AND FREE RADICAL SCAVENGING CAPACITY OF PHENOLIC EXTRACT FROM RUSSULA LAUROCERASI
Abstract
Objectives: Cellular damage caused by reactive oxygen species has been implicated in several diseases; hence antioxidants have significant importance in human health. The objective of this study was to evaluate antioxidant properties of phenolic extract from Russula laurocerasi and presence of components responsible for the activity.
Methods: The antioxidant properties  were studied using various in vitro assays. Qualitative determination of different bioactive constituents such as phenol, flavonoid, β-carotene, lycopene and ascorbic acid were also done.
Results: The extract was strong hydroxyl radical scavenger reflected by its low EC50 value i.e. 0.03 mg/ml. The EC50 values of the extract was in the order of hydroxyl radical scavenging< chelating ability of ferrous ion< DPPH scavenging< superoxide radical scavenging< β-carotene bleaching< reducing power. . The extract presented a relatively strong antioxidant effect which was found to be correlated with total phenols (R2= 0.969) and flavonoids (R2= 0.888) implying that the polyphenols was partly responsible for the antioxidant activities.
Conclusion: Our result thus indicates that the fraction of R. laurocerasi may be utilized as a promising source of therapeutics.
KEY WORDS: Antioxidant activity, edible mushroom, flavonoid, phenol, reactive oxygen species.
Downloads
References
Valko M, Leibfritz D, Moncol J, Cronin M T, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39: 44-84.
Chanda S, Dave R. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: an overview. Afr J Microbiol Res 2009; 3(13): 981-96.
Blokhina O, Virolainen E, Fagerstedt V. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 2003; 91: 179-94.
Khatua S, Paul S, Acharya K. Mushroom as the potential source of new generation of antioxidant: a review. Res J Pharm Technol 2013; 6(5): 496-505.
Manzi P, Pizzoferrato L. Beta-glucans in edible mushrooms. Food Chem 2000; 68: 315-8.
Mitra P, Khatua S, Acharya K. Free radical scavenging and NOS activation properties of water soluble crude polysaccharide from Pleurotus ostreatus. Asian J Pharm Clin Res 2013; 6(3): 67-70.
7. Chatterjee S, Biswas G, Chandra S, Saha GK, Acharya K. Apoptogenic effects of Tricholoma giganteum on Ehrlich’s ascites carcinoma cell. Bioprocess Biosyst Eng 2013; 36: 101-7.
8. Biswas G, Rana S, Sarkar S, Acharya K. Cardioprotective activity of ethanolic extract of Astraeus hygrometricus (Pers.) Morg. Pharmacologyonline 2011; 2: 808-17.
9. Biswas G, Acharya K. Hypoglycemic activity of ethanolic extract of Astraeus hygrometricus (Pers.) Morg. in alloxan-induced diabetic mice. Int J Pharm Pharm Sci 2013; 5: 391-4.
10. Acharya K. Medicinal properties of mushroom. In: Acharya SN, Thomas JE editors. Advances in Medicinal Plant Research. Kerala, India: Research Signpost; 2007. p. 215-36.
11. Acharya K, Chatterjee S, Biswas G, Chatterjee A, Saha GK. Hepatoprotective effect of a wild edible mushroom on carbon tetrachloride-induced hepatotoxicity in mice. Int J Pharm Pharm Sci 2012; 4: 285-8.
12. Chatterjee S, Datta R, Dey A, Pradhan P, Acharya K. In vivo hepatoprotective activity of ethanolic extract of Russula albonigra against carbon tetrachloride induced hepatotoxicity in mice. Res J Pharm Technol 2012; 5: 1034-8.
13. Giri S, Biswas G, Pradhan P, Mandal SC, Acharya K. Antimicrobial activities of basidiocarps of wild edible mushrooms of West Bengal, India. Int J Pharmtech Res 2012; 4: 1554-60.
14. Lai TK, Biswas G, Chatterjee S, Dutta A, Pal C, Banerji J et al. Leishmanicidal and anticandidal activity of constituents of Indian edible mushroom Astraeus hygrometricus. Chem Biodivers 2012; 9: 1517-24.
15. Chen X-H, Xia L-X, Zhou H-B, Qiu G-Z. Chemical Ccomposition and Aantioxidant Aactivities of Russula griseocarnosa sp. nov. J Agric Food Chem 2010; 58: 6966-71.
16. Yaltirak T, Aslima B, Ozturkb S, Alli H. Antimicrobial and antioxidant activities of Russula delica Fr. Food Chem Toxicol 2009; 47: 2052-6.
17. Das K, Sharma JR. Russulaceae of Kumaon Himalaya. Botanical Survey of India, Ministry of Environment and Forest, Kolkata; 2005; 176-228.
18. Cui Y, Kim D-S, Park K-C. Antioxidant effect of Inonotus obliquus. J Ethnopharmacol 2005; 96: 79-85.
19. Martinez AC, Marcelo EL, Marco AO, Moacyr M. Differential responses of superoxide dismutase in freezing resistant Solanum curtibolum and freezing sensitive Solanum tuberosum subjected to oxidative and water stress. Plant Sci 2001; 160: 505-15.
20. Halliwell B, Gutteridge JMC, Arumo OI. The deoxyribose method: Aa simple test tube assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 1987; 165: 215-9.
21. Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of Xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agri Food Chem 1992; 40: 945-8.
22. Dinis TCP, Mudaira VMC, Alnicida LM. Action of phenolic derivatives (acetaminophen, salicylate, and 5-amino salicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994; 315: 161-9.
23. Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986; 44: 307-15.
24. Dapkevicius A, Venskutonis R, Van Beek TA, Linssen PH. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. J Sci Food Agr 1998; 77: 140-6.
25. Singleton VL, Rossi Jr JA. Colorimetry of total phenolics with phosphomolybdio-phosphotungstic acid reagents. Am J Enol Viticult 1965; 16: 144-58.
26. Park YK, Koo MH, Ikegaki M, Contado JL. Comparison of the flavonoid aglycone contents of Apis mellifera propolis from various regions of Brazil. Arq Biol Tecnol 1997; 40: 97-106.
27. Nagata M, Yamashita I. Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit. Nippon Shokuhin Kogyo Gakkaishi 1992; 39: 925-8.
28. Rekha C, Poornima G, Manasa M, Abhipsa V, Pavithra DJ, Vijay KHT et al. Ascorbic acid, total phenol content and antioxidant activity of fresh juices of four ripe and unripe citrus fruits. Chem Sci Trans 2012; 1: 303-10.
29. Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005; 53: 1841-56.
30. Pal J, Ganguly S, Tahsin KS, Acharya K. In vitro free radical scavenging activity of wild edible mushroom, Pleurotus squarrosulus (Mont.) Singer. Indian J Exp Biol 2010; 48: 1210-8.
31. Dash S, Nath LK, Bhise S, Bhuyan N. Antioxidant and antimicrobial activities of Heracleum nepalense D Don root. Trop J Pharm Res 2005; 4: 341-7.
32. Waltasinghe M, Shahidi F. Antioxidant and free radical scavenging properties of ethanolic extracts of defatted borage (Berapa officinalis L.) seeds. Food Chem 1999; 63: 399-414.
33. Abah SE, Abah G. Antimicrobial and Aantioxidant Ppotentials of Agaricus bisporus. Adv Biol Res 2010; 4(5): 277-82.
34. Barros L, Ferreira M-J, Queirós B, Ferreira ICFR, Baptista P. Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem 2007; 103: 413-9.
35. Barros L, Falcãoa S, Baptistaa P, Freireb C, Vilas-Boasa M, Ferreira ICFR. Antioxidant activity of Agaricus sp. mushrooms by chemical, biochemical and electrochemical assays. Food Chem 2008; 111: 61-6.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.