A NOVEL WOUND DRESSING WITH SILKWORM COCOON SCAFFOLD TO FASTEN WOUND HEALING: AN IN VIVO STUDY USING RAT EXCISION WOUND MODEL

VALENTINA Y; JOHAN PANDIAN

doi:10.22159/ajpcr.2024v17i10.52201

Authors

VALENTINA Y Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Sri Balaji Vidyapeeth Deemed-to-be University, Pondicherry, India. https://orcid.org/0000-0002-4187-7402
JOHAN PANDIAN Department of Pharmacology, Mahatma Gandhi Medical College and Research Institute, Sri Balaji Vidyapeeth Deemed-to-be University, Pondicherry, India.

DOI:

https://doi.org/10.22159/ajpcr.2024v17i10.52201

Keywords:

Silver nanoparticles, Wound healing, Silkworm

Abstract

Objective: The study aimed to compare the effectiveness of silver-nano laden silkworm cocoon scaffold (SWCS), L-Ascorbic acid laden SWCS (AA-SWCS) over standard wet collagen sheet used for wound healing in rat incision wound model.

Methods: A total of 24 Wistar rats (of either sex, pathogen free, 10–12 weeks old) were used in this study. SWCS was prepared and an excision wound model was carried out to study the wound healing capacity in four study groups. Dressings were made with silver nanoparticles SWCS (AgNP-SWCS), AA-SWCS and compared with wet collagen sheet regularly. Friedman’s test was used for analysis.

Results: The results clearly indicate that both AgNP-SWCS and AA-SWCS significantly accelerate wound healing compared to the standard wet collagen sheet. The AgNP-SWCS outperformed the others in terms of wound contraction rate, histological quality of healing, and microbial load reduction. The AA-SWCS also showed enhanced healing properties, particularly in collagen synthesis and tissue organization.

Conclusion: The study suggests that both AgNP-SWCS and AA-SWCS are highly effective alternatives to traditional collagen dressings for wound healing. These innovative scaffolds could represent a new frontier in wound care, providing enhanced healing rates, improved tissue quality, and reduced infection risks, ultimately leading to better patient outcomes.

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References

Franci G, Falanga A, Galdiero S, Palomba L, Rai M, Morelli G, et al. Silver nanoparticles as potential antibacterial agents. Molecules. 2015;20(5):8856-74. doi: 10.3390/molecules20058856, PMID: 25993417

Atiyeh BS, Costagliola M, Hayek SN, Dibo SA. Effect of silver on burn wound infection control and healing: Review of the literature. Burns. 2007;33(2):139-48. doi: 10.1016/j.burns.2006.06.010, PMID: 17137719

Dahm H. Silver nanoparticles in wound infections: Present status and future prospects. In: Rai M, editor. Nanotechnology in Skin, Soft Tissue, and Bone Infections. Cham: Springer; 2020. p. 151-68.

Alavi M, Varma RS. Antibacterial and wound healing activities of silver nanoparticles embedded in cellulose compared to other polysaccharides and protein polymers. Cellulose. 2021;28(13):8295-311. doi: 10.1007/ s10570-021-04067-3

Singh M, Thakur V, Kumar V, Raj M, Gupta S, Devi N, et al. Silver nanoparticles and its mechanistic insight for chronic wound healing: Review on recent progress. Molecules. 2022;27(17):5587. doi: 10.3390/ molecules27175587, PMID: 36080353

Phillips CL, Combs SB, Pinnell SR. Effects of ascorbic acid on wound healing. J Plast Reconstr Surg. 1994;93(1):1047-51.

Dhabhai B, Sharma G. Role of vitamin C in wound healing. J Evol Med Dent Sci. 2013;2(2):278-84.

Thevi T, Abas AL, Rajan M. The effects of vitamin C on wound healin- -Systematic review. Indian J Surg. 2024;86(1):23-9. doi: 10.1007/ s12262-023-03750-y

Ghahremani-Nasab M, Del Bakhshayesh AR, Akbari-Gharalari N, Mehdipour A. Biomolecular and cellular effects in skin wound healing: The association between ascorbic acid and hypoxia-induced factor. J Biol Eng. 2023;17(1):62. doi: 10.1186/s13036-023-00380-6, PMID: 37784137

Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, et al. Silk-based biomaterials. Biomaterials. 2003;24(3):401-16. doi: 10.1016/ s0142-9612(02)00353-8, PMID: 12423595

Rockwood DN, Preda RC, Yücel T, Wang X, Lovett ML, Kaplan DL. Materials fabrication from Bombyx mori silk fibroin. Nat Protoc. 2011;6(10):1612-31. doi: 10.1038/nprot.2011.379, PMID: 21959241

Wu B, Tong X, Cheng L, Jiang S, Li Z, Li Z, et al. Hybrid membrane of flat silk cocoon and carboxymethyl chitosan formed through hot pressing promotes wound healing and repair in a rat model. Front Bioeng Biotechnol. 2022;10:1026876. doi: 10.3389/fbioe.2022.1026876, PMID: 36394047

Li G, Sun S. Silk Fibroin-based biomaterials for tissue engineering applications. Molecules. 2022 Apr 25;27(9):2757. doi: 10.3390/ molecules27092757, PMID: 35566110, PMCID: PMC9103528

Kim YJ, Kim SW, Kim KY, Ki CS, Um IC. Structural characteristics and properties of cocoon and regenerated silk fibroin from different silkworm strains. Int J Mol Sci. 2023;24(5):4965. doi: 10.3390/ ijms24054965, PMID: 36902396

Vyas KS, Vasconez HC. Wound healing: Biologics, skin substitutes, biomembranes and scaffolds. Healthcare (Basel). 2014;2(3):356-400. doi: 10.3390/healthcare2030356, PMID: 27429283

Sharma G, George Joy J, Sharma AR, Kim JC. Accelerated full-thickness skin wound tissue regeneration by self-crosslinked chitosan hydrogel films reinforced by oxidized CNC-AgNPs stabilized pickering emulsion for quercetin delivery. J Nanobiotechnology. 2024;22(1):323. doi: 10.1186/s12951-024-02596-0

Nosrati H, Aramideh Khouy R, Nosrati, A, Khodaei M, Banitalebi- Dehkordi M, Ashrafi-Dehkordi K, et al. Nanocomposite scaffolds for accelerating chronic wound healing by enhancing angiogenesis. J Nanobiotechnology. 2021;19(1):1. doi: 10.1186/s12951-020-00755- 7, PMID: 33397416

Mira M, Wibowo A, Tajalla GU, Cooper G, Da Silva Bartolo PJ, Barlian A. Osteogenic potential of a 3D printed silver nanoparticle-based electroactive scaffold for bone tissue engineering using human Wharton’s jelly mesenchymal stem cells. Mater Adv. 2023;4(23):6407- 18. doi: 10.1039/D3MA00332A

Boateng J, Catanzano O. Advanced therapeutic dressings for effective wound healing--A review. J Pharm Sci. 2015;104(11):3653-80. doi: 10.1002/jps.24610, PMID: 26308473