HPTLC PROFILE OF QUERCETIN IN THREE CULTIVARS OF Allium cepa AND ITS ANTIMICROBIAL ACTIVITY AGAINST BACTERIAL CULTURES
Abstract
Objective: This study was subjected to analyse the technique for the determination of quercetin, a flavonoid in onion (Allium cepa L.) from three different cultivars of bulb onion (O1, O2 and O3) which were extracted with petroleum ether, chloroform, methanol and water. Also this study was aimed to determine the antimicrobial activity of four solvent extracts of three onion cultivars against ten MTCC bacterial pathogens.
Methods: Quercetin in onion was determined by analytical technique, HPTLC and the antimicrobial activity was performed by well diffusion method.
Result: Quercetin was determined in all the three cultivars with Rf value 0.68. Quercetin was also extracted in all the four solvent extracts of onion cultivars. Apart from quercetin, few more compounds also isolated. More compounds were isolated in O3. In the determination of antimicrobial activity of onion extracts against MTCC cultures revealed that all the three onion cultivars were highly active against Staphylococcus aureus, it showed more than 25mm zone of inhibition. Among the three onion cultivars, O2 showed better antimicrobial activity against all the tested bacterial cultures.Â
Conclusion: This study showed that onion has good broad spectrum bioactivity against microorganisms revealed that it contains phytochemicals which can be used as neutraceuticals.
Keywords: Phytochemicals, High-performance thin layer chromatography, Onion, Quercetin, Antimicrobial activity, Microbial type culture collection
cultures.
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Shenoy C, Patil MB, Kumar R, Patil S. Preliminary phytochemical investigation and wound healing activity of Allium cepa Linn. (Liliaceae). Int J Pharm Pharm Sci 2009;2(2):167-75.
Pratt DE. Natural antioxidants from plant material. In: Ho CT, Lee CY, Huang M, editors. Phenolic Compounds in Food and their Effects on Health II; ACS Symposium Series 507. Washington, D.C: American Chemical Society; 1992. p. 54-71.
Duthie GG, Duthie SJ, Kyle JA. Plant polyphenols in cancer and heart disease: Implications as nutritional antioxidants. Nutr Res Rev 2000;13(1):79-106.
Stajner D, Milic N, Canadanovic-Brunet J, Kapor A, Stajner M, Popovic BM. Exploring Allium species as a source of potential medicinal agents. Phytother Res 2006;20(7):581-4.
Nencini C, Cavallo F, Capasso A, Franchi GG, Giorgio G, Micheli L. Evaluation of antioxidative properties of Allium species growing wild in Italy. Phytother Res 2007;21(9):874-8.
Sofija D, Vanja T, Silvana P, Jelena KM, Silva D, Marina M, et al. Bioactivity assays on Carlina acaulis and C. acanthifolia root and herb extracts. Dig J Nanomater Biostruct 2012;7(3):1213-22.
Aiyelaagbe OO, Osamudiamen PM. Phytochemical screening for active compounds in Mangifera indica leaves from Ibadan, Oyo state. Plant Sci Res 2009;2(1):11-3.
Nagore DH, Patail PS, Kuber VB. Comparison between high performance liquid chromatography and high performance thin layer chromatography determination of Diosgenin from Fenugreek seeds. Int J Green Pharm 2012;6(4):315-20.
Jain A, Lodhi S, Singhai AK. Simultaneous estimation of quercetin and rutin in Tephrosia purpurea Pers by high performance thin-layer chromatography. Asian J Tradit Med 2009;4(3):104-9.
Chakraborthy GS, Ghorpade PM. Determination of quercetin by HPTLC in Calendula officinalis extract. Int J Pharma Bio Sci 2010;1(1):1-4.
Sethi PD. HPTLC. 1st ed. New Delhi: CBS Publishers and Distributors; 1996. p. 39.
Mishra J, Singh RD, Jadon VS, Gusain MP, Aradhana P. HPTLC profile of quercetin content of common bean (Uttarakhand) landraces growing in Uttarakand. Am J Food Tech 2012;7(2):96-100.
Bandyopadhyay C, Srirangarajan AN, Sreenivasan A. Studies of flavour components of onion (Allium cepa). I. Thin-layer chromatographic investigation of onion. J Chromatogr 1970;47(3):400-7.
Itakura Y, Ichikawa M, Mori Y, Okino R, Udayama M, Morita T. How to distinguish garlic from the other Allium vegetables. Recent advances on the nutritional effects associated with the use of Garlic as a supplement. Am Soc Nutri Sci 2001;963s-7.
Parmar S, Shah N, Kasarwala M, Virpura M, Shah K, Patel P. Determination of quercetin by HPTLC method present in zymodyne syrup: A poly herbal formulation. Int J Pharm Sci Res 2011;2(10):2724‑8.
Ye CL, Dai DH, Hu WL. Antimicrobial and antioxidant activities of the essential oil from onion (Allium cepa L). Food Control 2013;30(1):48‑53.
Zohri AN, Abdel-Gawad K, Saber S. Antibacterial, anti dermatophytic and antitoxigenic activities of onion (Allium cepa L) oil. Microbiol Res 1995;150(2):167-72.
Adeshina GO, Jibo S, Agu VE, Ethinmidu JO. Antibacterial activity of fresh juices of Allium cepa and Zingiber officinale against multidrug resistant bacteria. Int J Pharm Biosci 2011;2(2):289-95.
Santas J, Pilar MA, Carbo R. Antimicrobial and antioxidant activity of crude onion (Allium cepa L.) extracts. Int J Food Sci Technol 2010;45(2):403-9.
Rekha J, Shruti D. Phytochemical extraction and antimicrobial activity of some medicinal plants on different microbial strains. J Med Plants Stud 2014;2(3):58-63.
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