Evaluation of Anti-inflammatory property of Melanin from marine Bacillus sp BTCZ31
Abstract
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Objectives: To evaluate the anti-inflammatory property of melanin from marine Bacillus spp. BTCZ31.
Methods: Radical scavenging property of melanin was determined by 2,2-Diphenyl-1-picrylhydrazyl and metal chelation assays, which was further
confirmed by electron paramagnetic resonance (EPR) spectroscopy. Anti-inflammatory property of melanin was explored in vitro in RAW264.7
cell line using cyclooxygenase (COX), Lipoxygenase (LOX), Myleoperoxidase (MPO), cellular nitrite inhibitory assays. Cytotoxicity of melanin was
determined using 3-(4,5 dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay.
Results: BTCZ31 melanin showed radical scavenging activity of 67.55% and ferrous ion chelating activity of 97.88%. EPR spectrum showed sharp
peaks indicating the presence of unpaired electrons. Melanin inhibited the activity of COX and LOX enzyme with IC50 values of 104.34 μg/mL and
10.5 μg/mL, respectively. It also reduced the activity of MPO and cellular nitrite levels. Cytotoxic concentration of melanin was found to be 105.4 μg/mL
(IC50).
Conclusion: Bacillus spp. BTCZ10 melanin can be a potential anti-inflammatory agent. Further in vivo evaluations are needed for confirming the
activity, leading to therapeutic applications.
Keywords: Anti-inflammatory, Antioxidant, Bacillus spp., Bacteria, Marine, Melanin.
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