Prevention of Insulin Resistance by Silymarin.
Keywords:
Dexamethasone, Silymarin, Insulin resistance, Fructose, Diabetes mellitusAbstract
Objective
The aim of this study was to evaluate the effect of silymarin on the dexamethasone and fructose – induced insulin resistance since its effect potential protective effects have been addressed in other models of cell damage induced by drugs.
Methods
Insulin resistance was induced by administration of either dexamethasone or fructose. In both model effect of concomitant administration of silymarin in two divided doses 100 and 200 mg/kg continued till end of the experiment were studied group 3-5, dexamethasone was administered to the overnight-fasted rats and continued till the end of the experiment along with silymarin 100 or 200 mg/kg p.o. At the end of the experimental period i.e. on day 11 in dexamethasone insulin resistance model, the serum glucose triglyceride and body weight was determined. Subsequently, insulin secretion in vitro from isolated perfused pancreas were determined On day 21 in fructose induced insulin resistance model the estimation of serum glucose, cholesterol triglyceride and insulin were done. Then the animals were sacrificed, a piece of liver was dissected out for determination of glycogen as per the method described by Montgomery.
Results
Silymarin 200 mg/kg treatment with Dexamethasone significantly inhibited the dexamethasone induced increase in serum glucose, triglyceride level, insulin secretion and reduction in body weight. After 21 days increase in serum glucose and insulin levels in fructose feeding rats was significantly decreased in group treated with Silymarin 200 mg/kg. Silymarin treatment to fructose fed rats increases glycogen levels of tissue compared to fructose control group
Conclusion
Silymarin might improve insulin resistance through enhanced insulin sensitivity in peripheral tissues. The results obtained in the present investigation indicate that silyamarin may have considerable therapeutic potential in the treatment of insulin resistance in NIDDM and its complications.
Keywords: Dexamethasone, Silymarin, Insulin resistance, fructose , Diabetes mellitus
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