GC-MS evaluation of bioactive phytochemicals of commercial green teas (Camellia sinensis) of India


  • Sivakumar T Assistant professor Department of Botany Annamalai University Chidambaram
  • Arulmozhi Kt Assistant ProfessorPhysics Wing DDEAnnamalai universityChidambaram
  • Mythili N Research Scholar Department of Physics Annamalai University Chidambaram


Objective: An attempt has been made to identify the presence and composition of bioactive phytochemcials present in six commercial green tea
(Camellia Sinensis) samples acquired from diverse locations of India. Methods: Gas chromatography-mass spectroscopy technique was used for
the qualitative and quantitative analysis of the phytochemicals present in the green tea samples. Results: The results indicated the presence of
various secondary metabolites like pyrogallol, quinic acid, caffeine, xanthine alkaloid palmitic acid, diterpene, linolenic acid in the studied samples.
Conclusion: The total peak area percentages of health beneficial phytochemicals in the green tea samples were in the range of 83 to 96.

Keywords: Gas chromatography-mass spectroscopy analysis, Phytochemical screening, Green tea, Bioactivity.


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Chin YW, Balunas MJ, Chai HB, Kinghorn AD. Drug discovery from natural sources. AAPS J 2006;8:E239-53.

Pcerangeli G, Vital G, Rivera W. Antimicrobial activity and cytotoxicity of Chromolaena odorata (L.f) king and rabinson and Uncaria perrottetii (A. Rich) Merr extracts. J Med Plants Res 2009;3(7):511-8.

Sermakkani M, Thangapandian V. GC-MS analysis of Cassia itallica leaf methanol extract. Asian J Pharm Clin Res 2012;5(2):90-4.

Marston A. Role of advances in chromatographic techniques in phytochemistry. Phytochemistry 2007;68(22-24):2786-98.

Konovalova O, Gergel E, Herhel V. GC-MS analysis of bioactive components of Shepherdia argentea (Pursh.). Nutt. from Ukranian Flora. Pharm Innov J 2013;2(6):7-12.

Kalaivani CS, Sathish SS, Janakiraman N, Johnson M. GC-MS studies on Andrographis paniculata (Burm. f.) Wall. Ex nees – A medicinally important plant. Int J Med Arom Plants 2012;2(1):69-74.

Praveenkumar P, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr J Biochem Res 2010;4(7):191-6.

Chibani S, Berhail-Boudouda H, Kabouche A, Aburaj T, Kabouche Z. Analysis of the essential oil of Ferula communis L. from Constantine Algeria. Int J Med Aromat Plants 2011;1(2):41-4.

Sangeetha J, Vijayalakshmi K. Determination of bioactive components of ethyl acetate fractions of Punica granatum rind extract. Int J Pharm Sci Drug Res 2011;3(2):116-22.

Ghannadi A, Dezfuly N. Essential oil analysis of the leaves of Persian true myrtle. Int J Med Aromat Plants 2011;1(2):48-50.

Venkataraman B, Samuel LA, Saradhi MP, Rao BN, Vamsi N, Krishna A, et al. Antibacterial, antioxidant activity and GC-MS analysis of Eupaturium odoratum. Asian J Pharm Clin Res 2012;5(2):99-106.

Namita P, Mukesh R, Vijay KJ. Camellia sinensis (Green tea): A review. Glob J Pharm 2012;6(2):52-9.

Hamilton-Miller JM. Antimicrobial properties of tea (Camellia sinensis L.). Antimicrob Agents Chemother 1995;39(11):2375-7.

Graham HN. Green tea composition, consumption, and polyphenol chemistry. Prev Med 1992;21(3):334-50.

Kulandhaivel M, Palaniswamy M. In vitro antimicrobial activity of Camellia sinensis and Myristica fragrans against Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans. Int J Pharm Biol Arch 2012;3(3):604-9.

Sinija VR, Mishra HN. Green tea: Health benefits (Review). J Nutr Environ Med 2008;17(4):232-42.

Mitsuhashi S, Saito A, Nakajima N, Shima H, Ubukata M. Pyrogallol structure in polyphenols in involved in apoptosis induction on HEK 293T and K562 cells. Molecules 2008;13(12):2998-3006.

Azam S, Hadi N, Khan NU, Hadi SM. Antioxidant and prooxidant properties of caffeine, theobrumine and xanthine. Med Sci Monit 2003;9(9):BR325-30.

Yu FL, Gapor A, Bender W. Evidence for the preventive effect of the polyunsaturated phytol side chain in tocotrienols on 17beta-estradiol epoxidation. Cancer Detect Prev 2005;29(4):383-8.

Inoue Y, Hada T, Shiraishi A, Hirose K, Hamashima H, Kobayashi S. Biphasic effects of geranylgeraniol, teprenone, and phytol on the growth of Staphylococcus aureus. Antimicrob Agents Chemother 2005;49(5):1770-4.

Patricio Santos CC, Salvadori MS, Mota VG, Costa LM, Almeida AA, Oliveira GA, et al. Antinociceptive and antioxidant activities of phytol in-vivo and in-vitro models. Neuroscience Journal, 2013;2013:1-9.

Grace OM, Light ME, Lindsey KL, Moholland DA, Staden JV, Jader AK. Antibacterial activity and isolation of antibacterial compounds from fruit of the traditional compounds from fruit of the traditional African medicinal plant Kigelia africana. S Afr J Bot 2002;68:220-2.

Pal M, Ghosh M. Prophylactic effect of a-linolenic acid and a-eleostearic acid against MeHg induced oxidative stress, DNA damage and structural changes in RBC membrane. Food Chem Toxicol 2012;50:2811-8.



How to Cite

SenthilKumar SR, S. T, A. Kt, and M. N. “GC-MS Evaluation of Bioactive Phytochemicals of Commercial Green Teas (Camellia Sinensis) of India”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 3, May 2015, pp. 278-82, https://journals.innovareacademics.in/index.php/ajpcr/article/view/5632.



Original Article(s)