GREEN SYNTHESIS OF SILVER NANOPARTICLES USING THE LEAF EXTRACT OF PUTRANJIVA ROXBURGHII WALL. AND THEIR ANTIMICROBIAL ACTIVITY.
Abstract
Â
Objective: This study deals with the synthesis of silver nanoparticles (AgNP's) from the extract of the leaves of the plant Putranjiva roxburghii wall.
Using biological method, i.e., green synthesis.
Methods: The extract from the leaves acts as a reducing and stabilizing agent for the AgNP's. Further characterization was done using various
techniques like ultraviolet (UV)-visible spectrophotometry, which shows surface plasmon resonance, Fourier transform infra-red spectroscopy
analysis shows formation of various bonds, scanning electron microscope (SEM) and transmission electron microscope (TEM) analysis depicts
the distribution and average size of nanoparticles. The antimicrobial activity was also checked against various bacteria and fungi using minimum
inhibitory concentration and well diffusion assay.
Result: UV analysis shows strong plasmon resonance between 420 and 480 nm SEM analysis shows the distribution of synthesized nanoparticles,
whereas TEM analysis shows the average particle size to be near about 5 nm and well diffusion assay proved that these nanoparticles are effective
against different microorganisms.
Conclusion: P. roxburghii wall. shows strong potential for the reduction of silver from Ag+ to Ag0 and nanoparticles so formed are strongly active
against various microorganism.
Keywords: Putranjiva roxburghii, Fourier transform infra-red, Scanning electron microscope, Transmission electron microscope.
Downloads
References
Farooqui MA, Chauhan PS, Krishnamoorthy P, Shaik J. Extraction of silver nano-particles from the leaf extracts of Clerodendrum inerme. Dig J Nanomater Biostruct 2010; 5:43-9.
Mohanpuria P, Yadav SK, Rana NK. Biosynthesis of nanoparticles: Technological concepts and future applications. J Nanopart Res 2008;10:507-17.
Wijnhoven SW, Peijnenburg WJ, Herberts CA, Hagens WI, Oomen AG, Heugens EH, et al. Nano-silver a review of available data and knowledge gaps in human and environmental risk assessment. Nanotoxicology 2009; 3:109-38.
Bhattacharya D, Gupta RK. Nanotechnology and potential of microorganisms. Crit Rev Biotechnol 2005;25(4):199-204.
Leela A, Vivekanandan M. Tapping the unexploited plant resources for the synthesis of silver nanoparticles. Afr J Biotechnol 2008;7:3162-5.
Awwad AM, Salem NM, Abdeen AO. Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity. Int J Ind Chem 2013;4:29.
Malarvizhi P, Ramakrishnan N. Biogenic silver nano particles using Calophyllum inophyllum leaf extract: Synthesis, spectral analysis and antimicrobial studies. World J Pharm Res 2014;3:2258-69.
Garg S, Chandra A, Mazumder A, Mazumder R. Analgesic potential of hydrogels of silver nanoparticles using aqueous extract of Saraca indica bark. Int J Pharm Sci Res 2014;5:240-5.
Mohan SC, Sasikala K, Anand T, Vengaiah PC, Krishnaraj S. Green synthesis, antimicrobial and antioxidant effects of silver nanoparticles using Canthium coromandelicum leaves extract. Res J Microbiol 2014;9:142-50.
Singh K, Panghal M, Kadyan S, Chaudhary U, Yadav JP. Green silver nanoparticles of Phyllanthus amarus: As an antibacterial agent against multi drug resistant clinical isolates of Pseudomonas aeruginosa. J Nanobiotechnology 2014;12(1):40.
Elumalai EK, Prasad TN, Kambala V, Nagajyothi PC, David E. Green synthesis of silver nanoparticle using Euphorbia Hirta L and their antifungal activities. Arch Appl Sci Res 2010;2(6):76-81.
Kaviya S, Santhanalakshmi J, Viswanathan B. Biosynthesis of silver nano-flakes by Crossandra infundibuliformis leaf extract. Mater lett 2012;67:64-6.
Sanpui P, Murugadoss A, Prasad PV, Ghosh SS, Chattopadhyay A. The antibacterial properties of a novel chitosan-Ag-nanoparticle composite. Int J Food Microbiol 2008 31;124(2):142-6.
Evanoff DD, Chumanov G. Size-controlled synthesis of nanoparticles. Measurement of extinction, scattering, and absorption cross sections. J Phys Chem B 2004;108:13957-62.
Percival SL, Bowler PG, Dolman J. Antimicrobial activity of silver-containing dressings on wound microorganisms using an in vitro biofilm model. Int Wound J 2007;4(2):186-91.
Chopra GR, Jain AC, Seshadri TR, Sood GR. Chemical components of leaves and root bark of Putranjiva roxburghii. Indian J Chem 1970;8:776-8.
Reanmongkol W, Noppapan T, Subhadhirasakul S. Antinociceptive, antipyretic, and anti-inflammatory activities of Putranjiva roxburghii Wall. Leaf extract in experimental animals. J Nat Med 2009;63(3):290‑6.
Bar H, Bhui DK, Sahoo GP, Sarkar P, De SP, Misra A. Green synthesis of silver nanoparticles using latex of Jatropha curcas. Colloids Surf A Physicochem Eng Asp 2009;339(1-3):134-9.
Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc 2008;3(2):163-75.
Shahwar D, Raza MA, Saeed A, Riasat M, Chattha FI, Javaid M, et al. Antioxidant potential of the extracts of Putranjiva roxburghii, Conyza bonariensis, Wood fordia fruiticosa and Senecio chrysanthemoids. Afr J Biotechnol 2012;11(18):4288-95.
Amit V, Jain SK, Shashi A. Hypoglycemic activity of Putranjiva roxburghii wall. Inalloxan induced diabetic rats. Int J Pharm Sci Res 2010;1(12):160-4.
Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, et al. Antimicrobial effects of silver nanoparticles. Nanomed Nanotechnol Biol Med 2007;3:95-10.
Li WR, Xie XB, Shi QS, Zeng HY, Ou-Yang YS, Chen YB. Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Appl Microbiol Biotechnol 2010;85(4):1115-22.
Li WR, Xie XB, Shi QS, Duan SS, Ouyang YS, Chen YB. Antibacterial effect of silver nanoparticles on Staphylococcus aureus. Biometals 2011;24(1):135-41.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.