DEVELOPMENT AND VALIDATION OF STABILITY INDICATING UPLC METHOD FOR QUANTIFICATION OF ANTI OXIDANT IN COMPLEX DOSAGE FORMS

Abstract

A simple, hi-sensitive, accurate, precise and specific UPLC method was developed for the identification and quantification of antioxidant from complex dosage forms. The chromatographic separation employs the isocratic elution by using C₁₈ column by using the mobile phase containing mixture of Methanol, Acetonitrile, Tetrahydrofuran and Buffer, with the flow rate of 0.4 mL/min. The analyte was detected and quantified at the wavelength of 291 nm by using photo diode array detector. The method was validated as per ICH guidelines and found to be specific, precise, linear and accurate. The peak purity of analyte with the aid of photo diode array detector was satisfactory. Hence this method was concluded that stability indicating method.

 

Key Words: Antioxidant, Complex Molecules, Stability Indicating UPLC method.