AN EXPERIMENTAL STUDY: USING PLANT FERTILIZER AS A POTENT CULTURE MEDIA FOR CHLORELLA VULGARIS
Abstract
During growth, living organisms absorb chemical elements from their environment in ratios as they occur in their tissues. These elements are absorbed as small molecules or as free ions, potentially affecting the relative ionic composition of their medium. To avoid these changes in the medium composition, the elements in culture media should be available in the same ratios as in which they occur in biological material. This paper showed how, by using liquid plant fertilizer as culture media was designed which approximate the average elemental composition of biological material. A preparation of green algae media such as Juller's media (as standard) and different concentration of liquid plant fertilizer (v/2V, v/4V,v/6V and v/8V) A comparative study was carried to estimate optical density, cell count and dry mass (Growth kinetics) by using spectrophotometer, hematocytometer (Neubauer, improved) and  dry weight method respectively. Results obtain showed maximum growth kinetics in chlorella vulgaris using liquid plant fertilizer media.Â
Downloads
References
Borowitzka MA (1994), In: Proceedings of age first Asia-Pacific conference on algal Biotechnology (Eds.) Phong, S.M., Lee, Y.K., Borowitzka M.A, and Whitton B.A., Univ. of Malaya, Kualalumpur, Pg 5- 15.
Castrday,K.,Z. Gombom & B. Szalonatai (1984) Manganese and cobalt toxicity in chlorophyll biosynthesis. Proc.nat.Acad.Sci. USA. Vol (81) Pg 476-478.
Finkle JB and Appleman D, The effect of magnesium concentration on growth of Chlorella. Plant Physiology, 664–673, (1952).
Glazer AN and Stryer L (1984), Applications of Phycoerythin, Trends Biochem. Sci. Vol(9) Pg 423-429.
Gerald C, Gerloff George P, Fitzgerald and Skoog F, The isolation purification and culture of blue- green algae. Ameri J ofBotany, 37: 216–218, (1950).
Hans E (1992), Staying Healthy with Nutrition. Berkeley (CA): Celestial Arts; Pg 307-8
Hantouch AA and Hreeb KK, The biochemical composition of some microalgal species isolated from the Shatt al- Arab River. Marina Mesopotamica, 18 (1): 1–8, (2003).
Jenson A (1978), Chlorophylls and carotenoids. In: Handbook of Psychological methods. Physiological and biochemistry methods. Hellebust JA and Crage JS (Eds.) Cambridge University Press, Cambridg. Pg.59-70.
Lustigman B., L.H.Lee & C. Weis-Magasic (1995) Effect of cobalt and pH on the growth of Chlamydomonas.reinhardtii.Bull.Environ.Contam.Toxicol. Vol (55) Pg 65-72
Lu. Chau & Zang J.H. (2000) Acute toxicity of excess mercury on the photosynthesis performance of cyanobacterium, Spirulina platensis –assesment by chlorophyll fluroscense analysis. Chemosphere,Vol (41) Pg 191-196.
Mallick N and Rai LC, Influence of culture density, pH, Organic acids and divalent cations on the removal of nutrients and metals by immobilized Anabaena doliolum
and Chlorella vulgaris. World J of Microbiology and Biotechnol, 9: 196– 201, (1993).
Mandalam R and Palsson BO, Elemental balancing of biomass and medium composition enhances growth capacity in high-density Chlorella vulgaris Cultures. Biotechnol Bioeng, 59: 605–611, (1998).
Parson TR and Strickland JDH (1965), particulate Organic matter. III. I. Pigment analysis. III. I. I. Determination of Phytoplankton pigments. J. Fish. Res. Bd. Canada, Vol (18) Pg 117-127
Prasad V, Kumar A and Kumar HD, Protective role of sodium chloride and sodium dithionite against UV- B induced damage in Chlorella vulgaris. Ecoprint, 13: 61–67, (2006).
Prask, J.A. &.Plocke D.J (1971) The role of zinc in the structural integrity of the cytoplasmic ribosomes of Euglena gracilis.Pl.Physiol.Vol (48) Pg 150-155
Price.N.M.,&. Morel.F.M.M (1990) .Cadmium and cobalt substitution for zinc in marine diatoms. Nature.Vol (344) Pg 656-660
Rodhe W, Environmental requirements of fresh water plankto algae. Symbolae Dot Upsal, 10: 1–149, (1948)
Round FE, The biology of the algae. University of Bristol 144, (1966).
Ramay C, Armesto J, Remrez D, Ganzolez R, Lendan N, Garcia I (1998), Antioxidant and anti-inflammatory properties of C-phycocyanin from blue green algae. Inflamm. Res. Vol (47) Pg 36-41.
Rai UN, Dwivedi S, Baghel VS, Tripathi RD, Shukla OP, and Shukla MK, Morphology and cultural behavior of Botryococcus protuberans with notes on the genus. J Environm Biol, 28 (2): 181–184, (2007
Sostaric M, Golob J, Bricelj M, Klinar D and Pivec A, Studies on the growth of Chlorella vulgaris in culture media with different carbon sources. Chem Biochem Eng, 4: 471–477, (2009).
Turpin DH, Growth rate dependent optimum ratios in Selenastrum minutum implication
for competition co-existence and stability in phytoplankton community. J Phycol, 22: 94–
, (1986).
Turpin DH, Growth rate dependent optimum ratios in Selenastrum minutum implication
for competition co-existence and stability in phytoplankton community. J Phycol, 22: 94–
, (1986).
Truog E (ed.), Mineral nutrition of plants. Univ. of Wisconsin Press Madison, (1951)
Wiesnner W, Inorganic micronutrients, In: R.A. Lewin (ed.), Physiology and biochemistry of algae. Academic Press New York, 267–286, (1962)
Wetherel D.F (1961) culture of fresh algae in enriched natural sea water. Physiol. Plant Vol 14 pg(1-6).
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.