ANTIULCER AND IN VITRO ANTIOXIDANT ACTIVITY OF ALLIUM HOOKERII: AN ETHNOMEDICINAL PLANT OF MANIPUR
Abstract
objective: To evaluate the antiulcer and antioxidant activity of the methanolic extract of Allium hookerii (MEAH) leaves.
Methods: In vivo antiulcer activity of MEAH was evaluated at two dose levels of 200 mg/kg and 400 mg/kg body weight by pyloric ligation, ethanol
(EtOH) induced ulcer and indomethacin-induced ulcer models through estimation of gastric contents viz. gastric volume, pH, free acidity, total acidity,
total hexoses, hexosamine, fucose and total protein. Antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay,
reducing power assay, superoxide radical scavenging activity, and hydrogen peroxide radical scavenging activity. Histopathology of the stomach was
studied using hematoxylin and eosin stained sections.
Results: A significant decrease in ulcer index and increase in percentage protection in ulcerated rats. After 7 days of treatment, the pH, free acidity
and total acidity decreased, thereby increased the content of total hexoses, hexosamine, fucose and total protein in the gastric content. MEAH showed
a significant antioxidative effect in DPPH, hydrogen peroxide radical scavenging activity and superoxide radical scavenging but showed very low effect
in reducing power assay. Histopathological studies of the stomach in ulcer and treated groups substantiate the cytoprotective action of extract in EtOH
induced ulcer animals.
Conclusions: It can be concluded from the research work that A. hookerii has potent antiulcer activity and supports the in vitro antioxidative status.
Keywords: Antisecretory, Antiulcer, Cytoprotection, Antioxidants, Allium hookerii.
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References
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