A REVIEW ON MATRIX ASSISTED LASER DESORPTION/INOZATION MASS SPECTROSCOPY
Abstract
ABSTRACT
Matrix assisted laser desorption ionization mass spectroscopy (MALDI-MS) is the most important technique of MS to analyze polymer systems. It is
a special case of MS using specific sample preparation methods and low fluence laser desorption to create the analyte ions. This technique is based
upon an ultraviolet absorbing matrix. The matrix and the polymer are mixed at a molecular level in an appropriate solvent. The solvent helps prevent
aggregation of the polymer. The sample matrix mixture is placed on the sample probe tip, under vacuum conditions; the solvent is removed, leaving cocrystallized
polymer molecules homogenously dispersed within matrix molecules. When the pulsed laser beam is tuned to the appropriate frequency,
the energy is transferred to the matrix which is partially vaporized, carrying intact polymer into the vapor phase and charging the polymer chains in
the linear time of flight (TOF) analyzer. This review includes the detailed information of MALDI-MS, MALDI-TOF.
Keywords: Matrix assisted laser desorption ionization mass spectroscopy, Principle, Sample preparation techniques, Matrix assisted laser desorption
ionization - time of flight, Matrix assisted laser desorption ionization - mass spectrometric imaging, Applications.
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References
. Available from: http://www.en.wikipedia.org/wiki/Matrix-assisted_
laser_desorption/ionization.
Marvin LF, Roberts MA, Fay LB. Matrix-assisted laser desorption/
ionization time-of-flight mass spectrometry in clinical chemistry. Clin
Chim Acta 2003;337(1-2):11-21.
Available from: http://www.chemwiki.ucdavis.edu ›...› Instrumental
Analysis › Mass Spectrometry.
de Hoffmann E, Stroobant V. Mass Spectroscopy Principles
and Applications. 3
ed. Chichester, UK: Wiley Publications;
p. 33-9.
rd
Jagtap RN, Ambre AH. Overview literature on matrix assisted laser
desorption ionization mass spectroscopy (MALDI MS): Basics and
its applications in characterizing polymeric materials. Indian Acad Sci
;28(6):515-28.
Karas M, Hillenkamp F. Laser desorption ionization of proteins
with molecular masses exceeding 10,000 daltons. Anal Chem
;60(20):2299-301.
Griffin TJ, Smith LM. Single-nucleotide polymorphism analysis
by MALDI–TOF mass spectrometry. Trends Biotechnol
;18(2):77-84.
Susnea I, Bernevic B, Wicke M, Ma L, Liu S, Schellander K, et al.
Application of MALDI-TOF-mass spectrometry to proteome analysis
using stain-free gel electrophoresis. Top Curr Chem 2013;331:37-54.
Karas M, Hillenkamp F. Int J Mass Spectrom 1987;78:53.
Belas FJ, Blair A, Ohannesian L, Streeter AJ. Handbook of
Asian J Pharm Clin Res, Vol 8, Issue 5, 2015, 28-33
Aparna et al.
Pharmaceutical Analysis. Ch. 4. New York, USA: Mass Spectrometry
in Pharmaceutical Analysis; 2001.
Timothy J Griffin, Smith, : Single-nucleotide polymorphism analysis
by MALDI–TOF mass spectrometry. Science direct Trends in bio
technologyFebruary 2000,(18), issue 2, p77 – 84.
IulianaSusnea, Bogdan Bernevic, Michael Wicke, Li Ma, Shuying
Liu, KarlSchellander, and Michael Przybylski Application of MALDITOF-Mass
Spectrometry
to Proteome Analysis
Using Stain-Free Gel
Electrophoresis.
Top
CurrChem 2013, (331), p 37–54.
Edmond de Hoffmann and Vincent Stroobant. Mass Spectroscopy
principles and applications: Wiley publications 3rd edition 2007, p
-9.
Frank J. Belas* and Ian A. Blair, Lena Ohannesian, Antony J. Streeter:
Handbook of Pharmaceutical Analysis: chapter 4, Mass Spectrometry
in Pharmaceutical Analysis.
Kristin J Boggio,1 Emmanuel Obasuyi,2 Ken Sugino,2 Sacha
B Nelson,2 Nathalie YR Agar,3 and Jeffrey N Agar, Expert Rev
Proteomics. 2011 Oct; 8(5): 591–604.
Angelini R1, Vitale R, Patil VA, Cocco T, Ludwig B, Greenberg ML,
Corcelli A, J Lipid Res. 2012 Jul;53(7):1417-25.
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