QUALITATIVE PHYTOCHEMICAL SCREENING AND EVALUATION OF IN-VITRO ANTIOXIDANT ACTIVITY OF CALLICARPA ARBOREA ROXB, AN ETHNOMEDICINAL PLANT OF MIZORAM, NORTHEAST INDIA
Abstract
ABSTRACT
Objective: The objective of the present study was to evaluate the phytochemical constitution and antioxidant activity of methanolic extract of dried leaves of Callicarpa arborea Roxb, an ethnomedicinal plant of Mizoram, Northeast India
Methods: Qualitative analysis of phytochemical constituents was performed by the well known tests protocol available in the literature on the methanolic leaves extract of the plant. Antioxidant activity was studied through DPPH and reducing power using BHT and ascorbic acid respectively as standards. The total phenolic and total flavonoid content were determined using Gallic acid and Quercetin respectively as standards.
Results: The phytochemical screening revealed the presence of saponin, steroids, glycosides, flavonoids and tannins. The methanol extract exhibited IC50 values of 2.393 µg/ml in DPPH assay. The reducing power of the extract was lower than that of the standard ascorbic acid. The total phenolic content and flavonoid content was 130.6 mg/g and 83.9 mg/g of gallic acid and quercetin equivalent respectively.
Conclusion: The results suggest that methanolic leaves extract of Callicarpa arborea has promising antioxidant activity and could serve as potential source of natural antioxidants.Â
Keywords: Callicarpa arborea, Phytochemicals, Antioxidant activity, Diphenyl-1-picrylhydrazyl, Phenol and flavonoid.
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References
Harvey AL. Natural Products in Drug Discovery. Drug Discovery today 2008;13:19-20.
Lam KS. New aspects of natural products in drug discovery. Trends in Microbiology 2007; 15 (6): 279-289.
Newman DJ. Natural products as sources of new drugs over the period 1981–2002. J. Nat. Prod. 2003;66:1022–1037.
Butler MS. The role of natural product chemistry in drug discovery. J. Nat. Prod 2004;67: 2141–2153.
Pawar NK, Arumugam N. Leaf extract of Centratherum punctatum exhibits antimicrobial, antioxidant and anti proliferative properties. Asian j pharm clin res 2011;4(3):7176
Uddin G, Rauf A, Qaisar M, Rehman TU, Latif A, Ali M. Preliminary phytochemical screening and antimicrobial activity of Hedera helix L. Middle-east journal of Scientific research 2011; 8(1):198-202.
Sahreen S, Khan MR, Khan RA. Evaluation of antioxidant activities of various solvent extracts of Carissa opaca fruits. Food Chemistry 2010;122:1205–1211.
Wu LC, Hsu HW, Chen YC, Chiu CC, Lin YI, Ho JA, Antioxidant and antiproliferative activities of red pitaya. Food Chemistry 2006;95:319–327.
Adom KK, Liu RH. Antioxidant Activity of Grains. Journal of Agricultural and Food Chemistry 2002;50:6182–6187.
Farombi EO, Hansen M, Ravn-Haren G, Moller P, Dragsted LO. Commonly consumed and naturally occurring dietary substances affect biomarkers of oxidative stress and DNA damage in healthy rats. Food and Chemical Toxicology 2004;42:1315–1322.
Soni A, Sosa S. Phytochemical Analysis and Free Radical Scavenging Potential of Herbal and Medicinal Plant Extracts. Journal of Pharmacognosy and Phytochemistry 2013;2(4): 22-29
Erkan N, Ayranci G, Ayranci E. Antioxidant activity of rosemary (Rosmarinus officinalis) extract, Black seed (Nigella sativa) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chemistry 2008;110:76-82.
Sre PRR, Sheila T, Murugesan K. Phytochemical screening and invitro antioxidant activity of methanolic root extract of Erythrina indica. Asian Pacific Journal of Tropical Biomedicine 2012;S1696-S1700
Rozika R. Ramhmul Damdawite (Medicinal Plants). 1st ed. Aizawl: Medicinal Plant Board Mizoram;2005.
Sharma HK, Chhangte L, Dolui AK. Traditional medicinal plants in Mizoram, India. Fitoterapia 2001;72:146-161.
Sen M, Pal BC. Chemical investigation of the bark of Callicarpa arborea (Verbenaceae) J Indian Chem Soc. 1974;51:903.
Sen M, Sarkar U. Chemical investigation of the leaves of Callicarpa arborea (Verbenaceae) J Indian Chem Soc. 1978;55:744–745.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 42nd ed. Pune: Nirali Prakashan; 2008.
Blois MS. Antioxidant determinations by the use of stable free radical. Nature 1958;181:1199-200.
Oyaizu M. Studies on products of browning reaction: Antioxidative activity of products of browning reaction. Jpn J Nutr 1986;40:307-15.
Mc donald S, Prenzler PD, Autolovich M, Robards K. Phenolic content and antioxidant activity of olive oil extracts. Food Chemistry 2001;73:73 84.
Chang C, Ming-Hua Y, Hwei-Mei W, Chuan Chen WJ. Determination of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.
Negi JS, Singh P, Rawat B. Chemical constituents and biological importance of Swertia: a review. Curr Res Chem 2011; 3:1-15.
Zahin M, AqilL F, Ahmad I. The in vitro antioxidant activity and total phenolic content of four indian medicinal plants. International Journal of Pharmacy and Pharmaceutical Sciences 2009;1(1):88-95.
Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity, Lebensmittel-Wissenschaftund-Technologie. Food Sci Technol 1995; 28:25-30.
Zhao H, Dong J, Lu J, Chen J, Li Y, Shan L et al. Effects of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley (Hordeum vulgare L.). J. Agric. Food Chem. 2006;54:7277–7286.
Oboh G, Akinyemi AJ, Ademiluyi AO. Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe(2+) induced lipid peroxidation in rat brain in vitro. Exp Toxicol Pathol. 2012;64(1-2):31-36.
Nithiyananthama S, Siddhurajua P, Francis G. A promising approach to enhance the total phenolic content and antioxidant activity of raw and processed Jatropha curcas L. kernel meal extracts. Industrial Crops and Products 2013;43:261–269.
Ka¨hko¨nen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS et al. Antioxidant Activity of Plant Extracts Containing Phenolic Compounds. J. Agric. Food Chem.1999;47: 3954-3962.
Salah N, Miler NJ, Pagange G, Tijburg L, Bolwell GP, Rice E, et al. Polyphenolic flavonoids as scavenger of aqueous phase radicals as chain breaking antioxidant. Arch Biochem Broph 1995;2:339-46.
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