QUANTIFICATION OF ANTIOXIDANT –PHYTOCHEMICAL STUDIES IN VITIS VINIFERA .L.VARIETIES
Abstract
objective: To quantify the antioxidant - Phytochemicals in Vitis vinifera varieties.
Methods: In this study, different antioxidant phytochemicals in V. vinifera varieties was determined in Thompson seedless, flame seedless, Kismis
chorni, Pusa navrang, and Rizamat by spectrophotometric method.
Results: Antioxidant is a compound that can delay or prevent the oxidation of lipids or other molecules by inhibiting the initiation or propagation
of an oxidizing chain reaction .The Quantification levels of various antioxidants in selected grape varieties which possess predominant quantities
of antioxidants are phenols, flavonols, flavonoids, ascorbic acid, anthocyanin, catalase, peroxidase, polyphenol oxidase, radical scavenging assay
(FRAP, ABTS, DPPH) respectively, phenols was recorded more in Rizamat (25.7±0.52 mg/g dry wt ), followed by FRAP radical scavenging activity
was maximum in Kismiss chorni variety (34±0.167µM/g dry wt ) among all the varieties Kismis chorni , Pusa navrang were showing maximum
antioxidant activity. Fruits have an important function in maintaining the physiological balance, optimal levels of antioxidants such as vitamin C
and phenol compounds ,Anthocyanin, , and phenol acids, being the most important for the human body .Vitamin C present in grapes, fights against
reactive oxygen species and polyphenols are associated with the prevention of diseases caused by oxidative stress. Phenolic content of these fruits
is significantly correlated with antioxidant capacity. These findings revealed that phytochemicals in the selected grapes have potent antioxidant
activities.
Conclusions: This study shows that maximum antioxidant activity levels were in Kismis chorni followe0d by other varieties. The present experiments
on Vitis varieties were designed to find out the important antioxidants and nutrient potential Antioxidants are vital substances which possess the
ability to protect the body from damage caused by free radical induced oxidative stress. From above study it is concluded that, Vitis vinifera promising
antioxidant and free radical scavenging activity. Thus can function as powerful free radical scavengers.
Keywords: Vitis vinifera, Antioxidant - Phytochemical studies, Antioxidant scavenging activity (ferric reducing antioxidant property, 2,2'-azinobis3ethylbenzthiazoline-sulphonic
acid, 2,2-Diphenyl
-2-picryl
hydrazyl),
Spectrophotometric
method.
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