ANTI-INFLAMMATORY AND ANALGESIC ACTIVITIES OF METHANOLIC SEED EXTRACT OF STERCULIA VILLOSA ROXB.
Abstract
objective: To scientifically validate the analgesic and anti-inflammatory properties of methanolic seed extract of Sterculia villosa Roxb. (SVME) in
animal models.
Methods: Analgesic activity of SVME was evaluated by the hot plate-induced pain model, acetic acid-induced abdominal writhing response, and
formalin test in mice at the doses of 250 and 500 mg/kg. A carrageenan-induced paw edema model was also used to evaluate anti-inflammatory
potential of SVME in rats at doses of 250 and 500 mg/kg.
Results: Phytochemical analysis revealed the presence of flavonoid, gums and carbohydrates, steroids, alkaloid, reducing sugar, and terpenoids in
significant amounts. The SVME produced significant analgesic activity in the hot-plate test in mice at all the time points measured. Extracts of 250 and
500 mg/kg reduced dose-dependent acetic acid-induced writhing by 40.2% (p<0.01) and 59.8% (p<0.001), respectively. Significant inhibition
of formalin-induced pain was also observed, with inhibition of 62.1% (p<0.001) and 66.7% (p<0.001) in the early phase at dosages of 250 and
500 mg/kg, respectively, and 64.4% (p<0.01) and 70.3% (p<0.01) in the late stage at these dosages. SVME pretreatments showed significant antiinflammatory
activity
against
carrageenan-induced
edema at all the time points measured.
Conclusion: The results suggest that SVME possesses analgesic and anti-inflammatory activities. These findings provide support use of this plant in
traditional medicine to treat pain and inflammation.
Keywords: Sterculia villosa Roxb., Analgesic, Anti-inflammatory, Carrageenan.
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References
Middleton E Jr, Kandaswami C, Theoharides TC. The effects of plant
flavonoids on mammalian cells: implications for inflammation, heart
disease, and cancer. Pharmacol Rev 2000;52(4):673-751.
Ricciotti E, FitzGerald GA. Prostaglandins and inflammation.
Arterioscler Thromb Vasc Biol 2011;31(5):986-1000.
Rang HP, Dale M, Ritter J. Pharmacology. 4th ed. New York: Churchill
Livingstone; 2001.
Wallace JL, Vong L. NSAID-induced gastrointestinal damage
and the design of GI-sparing NSAIDs. Curr Opin Investig Drugs
;9(11):1151-6.
Ibrahim B, Sowemimo A, van Rooyen A, Van de Venter M. Antiinflammatory,
analgesic
and antioxidant activities
of
Cyathula prostrata
(Linn.)
Blume (Amaranthaceae). J
Ethnopharmacol
;141(1):282-9.
Namsa ND, Tag H, Mandal M, Kalita P, Das AK. An ethnobotanical study
of traditional anti-inflammatory plants used by the Lohit community of
Arunachal Pradesh, India. J Ethnopharmacol 2009;125(2):234-45.
Kunwar RM, Shrestha KP, Bussmann RW. Traditional herbal medicine
in far-west Nepal: a pharmacological appraisal. J Ethnobiol Ethnomed
;6:35.
Ullah HM, Tareq SM, Huq I, Uddin MB, Salauddin MD. Antimicrobial
and cytotoxic activity assessment of the aqueous methanolic and
pet-ether extract of the leaves of Mesua ferrea. Int J Pharm Sci Res
;4(9):3563-8.
Ullah HM, Zaman S, Juhara F, Akter L, Tareq SM, Masum EH, et al.
Evaluation of antinociceptive, in-vivo and in-vitro anti-inflammatory
activity of ethanolic extract of Curcuma zedoaria rhizome. BMC
Complement Altern Med 2014;14:346.
Zimmermann M. Ethical guidelines for investigations of experimental
pain in conscious animals. Pain 1983;16(2):109-10.
Harborne JB. Phytochemical Methods: A Guide to Modern Techniques
of Plant Analysis. 3rd ed. London: Chapman and Hall; 1998.
Siddiqui S, Verma A, Rather AA, Jabeen F, Meghvansi MK. Preliminary
phytochemicals analysis of some important medicinal and aromatic
plants. Adv Biol Res 2009;3(5-6):188-95.
Lanhers MC, Fleurentin J, Mortier F, Vinche A, Younos C. Antiinflammatory
and
analgesic
effects
of
an
aqueous
extract
of
Harpagophytum
procumbens. Planta Med 1992;58(2):117-23.
Ojewole JA. Evaluation of the analgesic, anti-inflammatory and antidiabetic
properties
of
Sclerocarya birrea
(A. Rich.) Hochst.
stem-bark
aqueous
extract in mice and rats. Phytother Res 2004;18(8):601-8.
Koster R, Anderson M, De Beer EJ. Acetic acid for analgesic screening.
Fed Proc 1959;18:412-6.
Owoyele BV, Olaleye SB, Oke JM, Elegbe RA. Anti-inflammatory
and analgesic activities of leaf extracts of Landolphia owariensis. Afr J
Biomed Res 2001;4(3):131-3.
Altun ML, Çitoğlu GS, Yılmaz BS, Özbek H. Antinociceptive
and anti-inflammatory activities of Viburnum opulus. Pharm Biol
;47(7):653-8.
Mbagwu HO, Anene RA, Adeyemi OO. Analgesic, antipyretic and
anti-inflammatory properties of Mezoneuron benthamianum Baill
(Caesalpiniaceae). Nig Q J Hosp Med 2007;17(1):35-41.
Shibata M, Ohkubo T, Takahashi H, Inoki R. Modified formalin test:
characteristic biphasic pain response. Pain 1989;38(3):347-52.
Viana GS, do Vale TG, Rao VS, Matos FJ. Analgesic and antiinflammatory
effects
of two chemotypes
of Lippiaalba: A
comparative
study.
Pharm Biol 1998;36(5):347-51.
Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind
paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp
Biol Med 1962;111:544-7.
Ramesh M, Rao YN, Rao AV, Prabhakar MC, Rao CS, Muralidhar N,
et al. Antinociceptive and anti-inflammatory activity of a flavonoid
isolated from Caralluma attenuata. J Ethnopharmacol 1998;62(1):63-6.
Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant
flavonoids and cellular action mechanisms. J Pharmacol Sci
;96(3):229-45.
Asian J Pharm Clin Res, Vol 8, Issue 5, 2015, 287-291
Ullah et al.
Küpeli E, Yesilada E. Flavonoids with anti-inflammatory and antinociceptive
activity
from
Cistus laurifolius
L. leaves
through
bioassayguided
procedures. J
Ethnopharmacol
;112(3):524-30.
Middleton E Jr. Effect of plant flavonoids on immune and inflammatory
cell function. Adv Exp Med Biol 1998;439:175-82.
Li YD, Frenz CM, Chen MH, Wang YR, Li FJ, Luo C, et al. Primary
virtual and in vitro bioassay screening of natural inhibitors from
flavonoids against COX-2. Chin J Nat Med 2011;9(2):156-60.
Pathak D, Pathak K, Singla AK. Flavonoids as medicinal agents- Recent
advances. Fitoterapia 1991;62:371-85.
Pelzer LE, Guardia T, Osvaldo Juarez A, Guerreiro E. Acute and
chronic antiinflammatory effects of plant flavonoids. Farmaco
;53(6):421-4.
Barik BR, Bhowmik T, Dey AK, Patra A, Chatterjee A, Joy S, et al.
Premnazole, an isoxazole alkaloid of Premna integrifolia and Gmelina
arborea with anti-inflammatory activity. Fitoterapia 1992;63(4):295-9.
Chao J, Lu TC, Liao JW, Huang TH, Lee MS, Cheng HY, et al.
Analgesic and anti-inflammatory activities of ethanol root extract of
Mahonia oiwakensis in mice. J Ethnopharmacol 2009;125(2):297-303.
Calixto JB, Beirith A, Ferreira J, Santos AR, Filho VC, Yunes RA.
Naturally occurring antinociceptive substances from plants. Phytother
Res 2000;14(6):401-18.
Neukirch H, D’Ambrosio M, Sosa S, Altinier G, Della Loggia R,
Guerriero A. Improved anti-inflammatory activity of three new
terpenoids derived, by systematic chemical modifications, from the
abundant triterpenes of the flowery plant Calendula officinalis. Chem
Biodivers 2005;2(5):657-71.
Moody JO, Robert VA, Connolly JD, Houghton PJ. Anti-inflammatory
activities of the methanol extracts and an isolated furanoditerpene
constituent of Sphenocentrum jollyanum Pierre (Menispermaceae).
J Ethnopharmacol 2006;104(1-2):87-91.
Chapman CR, Casey KL, Dubner R, Foley KM, Gracely RH, Reading
AE. Pain measurement: an overview. Pain 1985;22(1):1-31.
Hargreaves K, Dubner R, Brown F, Flores C, Joris J. A new and sensitive
method for measuring thermal nociception in cutaneous hyperalgesia.
Pain 1988;32(1):77-88.
Collier HO, Dinneen LC, Johnson CA, Schneider C. The abdominal
constriction response and its suppression by analgesic drugs in the
mouse. Br J Pharmacol Chemother 1968;32(2):295-310.
Deraedt R, Jouquey S, Delevallée F, Flahaut M. Release of
prostaglandins E and F in an algogenic reaction and its inhibition. Eur J
Pharmacol 1980;61(1):17-24.
Berkenkopf JW, Weichman BM. Production of prostacyclin in mice
following intraperitoneal injection of acetic acid, phenylbenzoquinone
and zymosan: its role in the writhing response. Prostaglandins
;36(5):693-709.
Duarte ID, Nakamura M, Ferreira SH. Participation of the sympathetic
system in acetic acid-induced writhing in mice. Braz J Med Biol Res
;21(2):341-3.
Hunskaar S, Fasmer OB, Hole K. Formalin test in mice, a useful
technique for evaluating mild analgesics. J Neurosci Methods
;14(1):69-76.
Hunskaar S, Hole K. The formalin test in mice: dissociation between
inflammatory and non-inflammatory pain. Pain 1987;30(1):103-14.
Tjølsen A, Berge OG, Hunskaar S, Rosland JH, Hole K. The formalin
test: an evaluation of the method. Pain 1992;51(1):5-17.
Abbott FV, Franklin KB, Westbrook RF. The formalin test: scoring
properties of the first and second phases of the pain response in rats.
Pain 1995;60(1):91-102.
Ahmadiani A, Fereidoni M, Semnanian S, Kamalinejad M, Saremi S.
Antinociceptive and anti-inflammatory effects of Sambucus ebulus
rhizome extract in rats. J Ethnopharmacol 1998;61:229-35.
Chen YF, Tsai HY, Wu TS. Anti-inflammatory and analgesic activities
from roots of Angelica pubescens. Planta Med 1995;61(1):2-8.
Morris CJ. Carrageenan-induced paw edema in the rat and mouse.
Methods Mol Biol 2003;225:115-21.
Salvemini D, Wang ZQ, Wyatt PS, Bourdon DM, Marino MH,
Manning PT, et al. Nitric oxide: a key mediator in the early and late
phase of carrageenan-induced rat paw inflammation. Br J Pharmacol
;118(4):829-38.
Seibert K, Zhang Y, Leahy K, Hauser S, Masferrer J, Perkins W,
et al. Pharmacological and biochemical demonstration of the role of
cyclooxygenase 2 in inflammation and pain. Proc Natl Acad Sci U S A
;91(25):12013-7.
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